| Low PSI content limits the photoprotection of PSI and PSII in early growth stages of chlorophyll b-deficient wheat mutant lines | |
| Brestic, M; Zivcak, M; Kunderlikova, K; Sytar, O; Shao, HB; Kalaji, H; Allakhverdiev, S; Kalaji, H (reprint author), Warsaw Agr Univ SGGW, Fac Agr & Biol, Dept Plant Physiol, Nowoursynowska 159, PL-02776 Warsaw, Poland. marian.brestic@uniag.sk; marek.zivcak@uniag.sk; xkunderlikov@is.uniag.sk; o_sytar@ukr.net; hazem@kalaji.pl; suleyman.allakhverdiev@gmail.com | |
| 发表期刊 | PHOTOSYNTHESIS RESEARCH
 |
| 2015-08-01 | |
| 卷号 | 125期号:1-2页码:151-166 |
| 关键词 | Chlorina Mutants Wheat Non-photochemical Quenching Chlorophyll Fluorescence Transthylakoid Proton Gradient Psi Photoinhibition |
| 产权排序 | [Brestic, Marian; Zivcak, Marek; Kunderlikova, Kristyna; Sytar, Oksana; Shao, Hongbo] Slovak Univ Agr, Dept Plant Physiol, Nitra 94976, Slovakia; [Sytar, Oksana] Taras Shevchenko Natl Univ Kyiv, Dept Plant Physiol & Ecol, UA-01601 Kiev, Ukraine; [Shao, Hongbo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Kalaji, Hazem M.] Warsaw Agr Univ SGGW, Fac Agr & Biol, Dept Plant Physiol, PL-02776 Warsaw, Poland; [Allakhverdiev, Suleyman I.] Russian Acad Sci, Inst Plant Physiol, Moscow 127276, Russia; [Allakhverdiev, Suleyman I.] Russian Acad Sci, Inst Basic Biol Problems, Moscow 142290, Russia; [Allakhverdiev, Suleyman I.] Moscow MV Lomonosov State Univ, Dept Plant Physiol, Fac Biol, Moscow 119991, Russia |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | In vivo analyses of electron and proton transport-related processes as well as photoprotective responses were carried out at different stages of growth in chlorophyll b (Chl b)-deficient mutant lines (ANK-32A and ANK-32B) and wild type (WT) of wheat (Triticum aestivum L.). In addition to a high Chl a-b ratio, ANK mutants had a lower content of photo-oxidizable photosystem I (PSI, P (m)), and several parameters indicated a low PSI/PSII ratio. Moreover, simultaneous measurements of Chl fluorescence and P700 indicated a shift of balance between redox poise of the PSII acceptor side and the PSII donor side, with preferential reduction of the plastoquinone pool, resulting in an over reduced PSI acceptor side (high I broken vertical bar (NA) values). This was the probable reason for PSI inactivation observed in the ANK mutants, but not in WT. In later growth phases, we observed partial relief of "chlorina symptoms," toward WT. Measurements of Delta A (520) decay confirmed that, in early growth stages, the ANK mutants with low PSI content had a limited capacity to build up the transthylakoid proton gradient (Delta pH) needed to trigger non-photochemical quenching (NPQ) and to regulate the electron transport by cytochrome b (6)/f. Later, the increase in the PSI/PSII ratio enabled ANK mutants to reach full NPQ, but neither over reduction of the PSI acceptor side nor PSI photoinactivation due to imbalance between the activity of PSII and PSI was mitigated. Thus, our results support the crucial role of proper regulation of linear electron transport in the protection of PSI against photoinhibition. Moreover, the ANK mutants of wheat showing the dynamic developmental changes in the PSI/PSII ratio are presented here as very useful models for further studies.; In vivo analyses of electron and proton transport-related processes as well as photoprotective responses were carried out at different stages of growth in chlorophyll b (Chl b)-deficient mutant lines (ANK-32A and ANK-32B) and wild type (WT) of wheat (Triticum aestivum L.). In addition to a high Chl a-b ratio, ANK mutants had a lower content of photo-oxidizable photosystem I (PSI, P (m)), and several parameters indicated a low PSI/PSII ratio. Moreover, simultaneous measurements of Chl fluorescence and P700 indicated a shift of balance between redox poise of the PSII acceptor side and the PSII donor side, with preferential reduction of the plastoquinone pool, resulting in an over reduced PSI acceptor side (high I broken vertical bar (NA) values). This was the probable reason for PSI inactivation observed in the ANK mutants, but not in WT. In later growth phases, we observed partial relief of "chlorina symptoms," toward WT. Measurements of Delta A (520) decay confirmed that, in early growth stages, the ANK mutants with low PSI content had a limited capacity to build up the transthylakoid proton gradient (Delta pH) needed to trigger non-photochemical quenching (NPQ) and to regulate the electron transport by cytochrome b (6)/f. Later, the increase in the PSI/PSII ratio enabled ANK mutants to reach full NPQ, but neither over reduction of the PSI acceptor side nor PSI photoinactivation due to imbalance between the activity of PSII and PSI was mitigated. Thus, our results support the crucial role of proper regulation of linear electron transport in the protection of PSI against photoinhibition. Moreover, the ANK mutants of wheat showing the dynamic developmental changes in the PSI/PSII ratio are presented here as very useful models for further studies. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CYCLIC ELECTRON FLOW ; LIGHT-HARVESTING COMPLEX ; I SUBMEMBRANE FRACTIONS ; WATER-WATER CYCLE ; PHOTOSYSTEM-I ; FLUORESCENCE RATIO ; CHLORINA MUTANTS ; ANTENNA SIZE ; PHOTOSYNTHETIC CHARACTERISTICS ; ARABIDOPSIS-THALIANA |
| 研究领域[WOS] | Plant Sciences |
| WOS记录号 | WOS:000356311900014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9259 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Kalaji, H (reprint author), Warsaw Agr Univ SGGW, Fac Agr & Biol, Dept Plant Physiol, Nowoursynowska 159, PL-02776 Warsaw, Poland. marian.brestic@uniag.sk; marek.zivcak@uniag.sk; xkunderlikov@is.uniag.sk; o_sytar@ukr.net; hazem@kalaji.pl; suleyman.allakhverdiev@gmail.com |
| 推荐引用方式 GB/T 7714 | Brestic, M,Zivcak, M,Kunderlikova, K,et al. Low PSI content limits the photoprotection of PSI and PSII in early growth stages of chlorophyll b-deficient wheat mutant lines[J]. PHOTOSYNTHESIS RESEARCH,2015,125(1-2):151-166. |
| APA | Brestic, M.,Zivcak, M.,Kunderlikova, K.,Sytar, O.,Shao, HB.,...&suleyman.allakhverdiev@gmail.com.(2015).Low PSI content limits the photoprotection of PSI and PSII in early growth stages of chlorophyll b-deficient wheat mutant lines.PHOTOSYNTHESIS RESEARCH,125(1-2),151-166. |
| MLA | Brestic, M,et al."Low PSI content limits the photoprotection of PSI and PSII in early growth stages of chlorophyll b-deficient wheat mutant lines".PHOTOSYNTHESIS RESEARCH 125.1-2(2015):151-166. |
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