DNA nanostructure-based magnetic beads for potentiometric aptasensing
Ding, JW; Gu, Y; Li, F; Zhang, HX; Qin, W; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
2015
Source PublicationAnalytical Chemistry
Issue87Pages:6465
Contribution Rank[Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
AbstractIn this work, a simple, general, and sensitive potentiometric platform is presented, which allows potentiometric sensing to be applied to any class of molecule irrespective of the analyte charge. DNA nanostructures are self-assembled on magnetic beads via the incorporation of an aptamer into a hybridization chain reaction. The aptamer target binding event leads to the disassembly of the DNA nanostructures, which results in a dramatic change in the surface charge of the magnetic beads. Such a surface charge change can be sensitively detected by a polycation-sensitive membrane electrode using protamine as an indicator. With an endocrine disruptor bisphenol A as a model, the proposed potentiometric method shows a wide linear range from 0.1 to 100 nM with a low detection limit of 80 pM (3 sigma). The proposed sensing strategy will lay a foundation for the development of potentiometric sensors for highly sensitive and selective detection of various targets.
Department中科院海岸带环境过程与生态修复重点实验室
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8945
Collection中科院海岸带环境过程与生态修复重点实验室_环境化学实验室
Corresponding AuthorQin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
Recommended Citation
GB/T 7714
Ding, JW,Gu, Y,Li, F,et al. DNA nanostructure-based magnetic beads for potentiometric aptasensing[J]. Analytical Chemistry,2015(87):6465.
APA Ding, JW,Gu, Y,Li, F,Zhang, HX,Qin, W,&Qin, W .(2015).DNA nanostructure-based magnetic beads for potentiometric aptasensing.Analytical Chemistry(87),6465.
MLA Ding, JW,et al."DNA nanostructure-based magnetic beads for potentiometric aptasensing".Analytical Chemistry .87(2015):6465.
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