YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin
Zhang, Zhong1,3; Li, Jinhua1; Fu, Junqing1,2; Chen, Lingxin1; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
2014
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume4Issue:40Pages:20677-20685
Contribution Rank[Zhang, Zhong; Li, Jinhua; Fu, Junqing; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Fu, Junqing] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China; [Zhang, Zhong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
AbstractMolecular imprinting as a versatile technology is emerging for diverse species in various fields; however protein imprinting faces several problems related to the size, structural complexity, conformational flexibility, and compatibility with solvents. Herein, by using phycocyanin as a model, with physiological significance and fluorescence characteristics, we developed a facile and highly efficient approach to obtain fluorescent and magnetic dual-responsive coreshell imprinting microspheres. Twostage miniemulsion polymerization was employed, based on surface immobilization of phycocyanin with aminolysis and aldehyde modification on superparamagnetic support particles. The dual-responsive imprinting microspheres exhibited high adsorption capacity of 10.53 mg g(-1), excellent binding selectivity toward phycocyanin with a high imprinting factor of 2.41, and good reproducibility with standard error within 10%. Furthermore, fast simple magnetic separation and sensitive fluorescent detection in a wide pH range was offered for phycocyanin, showing a good linearity within 0.01-1.0 mg L-1 (R-2 = 0.9970) and a favorable detectability up to 1.5 ng mL(-1). Consequently, the imprinting microspheres were successfully applied as sorbents for selective isolation of phycocyanin from protein mixtures and special imaging recognition. Taking advantages of dual-responsive polymers and surface imprinting, the developed strategy provides great application potentials for convenient, rapid targeting identification/enrichment and separation of proteins and thereby contributing to targeting drug delivery and protein research.; Molecular imprinting as a versatile technology is emerging for diverse species in various fields; however protein imprinting faces several problems related to the size, structural complexity, conformational flexibility, and compatibility with solvents. Herein, by using phycocyanin as a model, with physiological significance and fluorescence characteristics, we developed a facile and highly efficient approach to obtain fluorescent and magnetic dual-responsive coreshell imprinting microspheres. Twostage miniemulsion polymerization was employed, based on surface immobilization of phycocyanin with aminolysis and aldehyde modification on superparamagnetic support particles. The dual-responsive imprinting microspheres exhibited high adsorption capacity of 10.53 mg g(-1), excellent binding selectivity toward phycocyanin with a high imprinting factor of 2.41, and good reproducibility with standard error within 10%. Furthermore, fast simple magnetic separation and sensitive fluorescent detection in a wide pH range was offered for phycocyanin, showing a good linearity within 0.01-1.0 mg L-1 (R-2 = 0.9970) and a favorable detectability up to 1.5 ng mL(-1). Consequently, the imprinting microspheres were successfully applied as sorbents for selective isolation of phycocyanin from protein mixtures and special imaging recognition. Taking advantages of dual-responsive polymers and surface imprinting, the developed strategy provides great application potentials for convenient, rapid targeting identification/enrichment and separation of proteins and thereby contributing to targeting drug delivery and protein research.
KeywordSolid-phase Extraction Drug-delivery C-phycocyanin Polymers Protein Nanoparticles Surface Separation Samples
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaChemistry, Multidisciplinary
DOI10.1039/c4ra00668b
Funding OrganizationChemistry
Indexed BySCI
WOS KeywordSOLID-PHASE EXTRACTION ; DRUG-DELIVERY ; C-PHYCOCYANIN ; POLYMERS ; PROTEIN ; NANOPARTICLES ; SURFACE ; SEPARATION ; SAMPLES
SubtypeArticle
Language英语
WOS Research AreaChemistry
WOS IDWOS:000336833400011
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8786
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorChen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Zhong,Li, Jinhua,Fu, Junqing,et al. Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin[J]. RSC ADVANCES,2014,4(40):20677-20685.
APA Zhang, Zhong,Li, Jinhua,Fu, Junqing,Chen, Lingxin,&Chen, LX .(2014).Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin.RSC ADVANCES,4(40),20677-20685.
MLA Zhang, Zhong,et al."Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin".RSC ADVANCES 4.40(2014):20677-20685.
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