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Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals
Zhang, Yingying1,3; Wang, Qing1; Ji, Yinglu2; Zhang, Qian4; Wu, Huifeng1; Xie, Jia1,3; Zhao, Jianmin1; Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Re, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
2014-05-01
Source PublicationENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
ISSN1382-6689
Volume37Issue:3Pages:1243-1255
Contribution Rank[Zhang, Yingying; Wang, Qing; Wu, Huifeng; Xie, Jia; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Re, Yantai 264003, Peoples R China; [Ji, Yinglu] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; [Zhang, Yingying; Xie, Jia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Qian] China Agr Univ, Yantai 264670, Peoples R China
Abstract17 beta-Hydroxysteroid dehydrogenases (17 beta-HSDs) are multifunctional enzymes involved in the metabolism of steroids, fatty acids, retinoids and bile acid. In this study, two novel types of 17 beta-HSDs (named as MgHsd17b10 and MgHsd17b12) were cloned from Mytilus galloprovincialis by using rapid amplification of cDNA ends (RACE) approaches. Sequence analysis showed that MgHsd17b10 and MgHsd17b12 encoded a polypeptide of 259 and 325 amino acids, respectively. Phylogenetic analysis revealed that MgHsd17b10 and MgHsd17b12 were evolutionarily clustered with other invertebrate 17 beta-HSD type 10 and 17 beta-HSD type 12 homologues. The MgHsd17b10 and MgHsd17b12 transcripts could be detected in all examined tissues with higher expression levels in digestive glands and gonad. After exposed to endocrine disrupting chemicals (Bisphenol A or 2,2',4,4'-tetrabromodiphenyl ether), the expression of MgHsd17b10 and MgHsd17b12 transcripts was both down-regulated in digestive glands. These findings suggest that MgHsd17b10 and MgHsd17b12 perhaps play an important role in the endocrine regulation of M. galloprovincialis. (C) 2014 Elsevier B.V. All rights reserved.; 17 beta-Hydroxysteroid dehydrogenases (17 beta-HSDs) are multifunctional enzymes involved in the metabolism of steroids, fatty acids, retinoids and bile acid. In this study, two novel types of 17 beta-HSDs (named as MgHsd17b10 and MgHsd17b12) were cloned from Mytilus galloprovincialis by using rapid amplification of cDNA ends (RACE) approaches. Sequence analysis showed that MgHsd17b10 and MgHsd17b12 encoded a polypeptide of 259 and 325 amino acids, respectively. Phylogenetic analysis revealed that MgHsd17b10 and MgHsd17b12 were evolutionarily clustered with other invertebrate 17 beta-HSD type 10 and 17 beta-HSD type 12 homologues. The MgHsd17b10 and MgHsd17b12 transcripts could be detected in all examined tissues with higher expression levels in digestive glands and gonad. After exposed to endocrine disrupting chemicals (Bisphenol A or 2,2',4,4'-tetrabromodiphenyl ether), the expression of MgHsd17b10 and MgHsd17b12 transcripts was both down-regulated in digestive glands. These findings suggest that MgHsd17b10 and MgHsd17b12 perhaps play an important role in the endocrine regulation of M. galloprovincialis. (C) 2014 Elsevier B.V. All rights reserved.
Keyword17 Beta-hydroxysteroid Dehydrogenases Mytilus Galloprovincialis Digestive Glands Endocrine Disrupting Chemicals Gene Expression
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaEnvironmental Sciences ; Pharmacology & Pharmacy ; Toxicology
DOI10.1016/j.etap.2014.04.027
Funding OrganizationEnvironmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
Indexed BySCI
WOS KeywordCHROMATOGRAPHY-MASS SPECTROMETRY ; POLYBROMINATED DIPHENYL ETHERS ; ESTROGEN-RECEPTOR-ALPHA ; BISPHENOL-A ; IN-VITRO ; HYDROXYSTEROID DEHYDROGENASES ; DIALLYL PHTHALATE ; CLONING ; VITELLOGENIN ; BETA
SubtypeArticle
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
WOS IDWOS:000338598200040
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8749
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorZhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Re, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Re, Yantai 264003, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.China Agr Univ, Yantai 264670, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Yingying,Wang, Qing,Ji, Yinglu,et al. Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2014,37(3):1243-1255.
APA Zhang, Yingying.,Wang, Qing.,Ji, Yinglu.,Zhang, Qian.,Wu, Huifeng.,...&jmzhao@yic.ac.cn.(2014).Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,37(3),1243-1255.
MLA Zhang, Yingying,et al."Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 37.3(2014):1243-1255.
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