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Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine
Lei, Jiahong; Ding, Jiawang; Chen, Yan; Qin, Wei; Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
2015-02-09
Volume858Pages:60-65
KeywordPotentiometry Polycation-sensitive Membrane Electrodes Protamine Heparin Current-controlled Release Flow Injection Analysis
DOI10.1016/j.aca.2014.12.018
Contribution Rank[Lei, Jiahong; Ding, Jiawang; Chen, Yan; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Lei, Jiahong; Ding, Jiawang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department中科院海岸带环境过程与生态修复重点实验室
AbstractA flow injection system incorporated with a polycation-sensitive polymeric membrane electrode in the flow cell is proposed for potentiometric determination of heparin. An external current in nano-ampere scale is continuously applied across the polymeric membrane for controlled release of protamine from the inner filling solution to the sample solution, which makes the electrode membrane regenerate quickly after each measurement. The protamine released at membrane-sample interface is consumed by heparin injected into the flow cell via their strong electrostatic interaction, thus decreasing the measured potential, by which heparin can be detected. Under optimized conditions, a linear relationship between the potential peak height and the concentration of heparin in the sample solution can be obtained in the range of 0.1-2.0 U mL (1), and the detection limit is 0.06 U mL (1). The proposed potentiometric sensing system has been successfully applied to the determination of heparin in undiluted sheep whole blood. (C) 2014 Elsevier B.V. All rights reserved.
Subtype期刊论文
Indexed BySCI
Language英语
WOS KeywordSENSITIVE MEMBRANE ELECTRODES ; MOLECULAR-WEIGHT HEPARIN ; POLYION SENSORS ; ELECTROCHEMICAL SENSOR ; RESPONSE MECHANISM ; WHOLE-BLOOD ; LABEL-FREE ; POLYANIONS ; SURGERY
WOS Research AreaInstrument Developing Project of the Chinese Academy of Sciences [YZ201161] ; National Natural Science Foundation of China [41176081, 21207156] ; Taishan Scholar Program of Shandong Province
WOS IDWOS:000348038600008
Funding OrganizationChemistry
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8655
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorQin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
Recommended Citation
GB/T 7714
Lei, Jiahong,Ding, Jiawang,Chen, Yan,et al. Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine[J]. ANALYTICA CHIMICA ACTA,2015,858:60-65.
APA Lei, Jiahong,Ding, Jiawang,Chen, Yan,Qin, Wei,&Qin, W .(2015).Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine.ANALYTICA CHIMICA ACTA,858,60-65.
MLA Lei, Jiahong,et al."Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine".ANALYTICA CHIMICA ACTA 858(2015):60-65.
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