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Ultrasensitive colorimetric detection of Cu2+ ion based on catalytic oxidation of L-cysteine
Yin, Kun; Li, Bowei; Wang, Xiaochun; Zhang, Weiwei; Chen, Lingxin; Zhang, WW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. wwzhang@yic.ac.cn; lxchen@yic.ac.cn
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
2015-02-15
Volume64Pages:81-87
KeywordCu2++ Ion Catalytic Oxidation L-cysteine Colorimetric Detection
DOI10.1016/j.bios.2014.08.058
Contribution Rank[Yin, Kun; Li, Bowei; Zhang, Weiwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaochun] Minist Land & Resources, Natl Res Ctr Geoanal, Key Lab Ecol Geochem, Beijing 100037, Peoples R China; [Yin, Kun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department中科院海岸带环境过程与生态修复重点实验室
AbstractAs an essential element, copper ion (Cu2+) plays important roles in human beings for its participation in diverse metabolic processes as a cofactor and/or a structural component of enzymes. However, excessive uptake of Cu2+ ion gives rise to the risk of certain diseases. So, it is important to develop simple ways to monitor and detect Cu2+ ion. In this study, a simple, facile colorimetric sensor for the ultrasensitive determination of Cu2+ ion was developed based on the following principle: L-cysteine and 1-chloro-2,4-dinitrobenzene (CDNB) could be conjugated to form the yellow product 2,4-dinitrophenylcysteine (DNPC), which was measurable at 355 nm; however, upon addition of Cu2+ ion, the absorbance of DNPC would be decreased owing to the Cu2+ ion catalytic oxidation of L-cysteine to L-cystine in the presence of O-2. Thus, the colorimetric detection of Cu2+ ion could be achieved. The optimal pH, buffer, temperature and incubation time for the colorimetric sensor were obtained of pH 6.8 in 0.1 M HEPES solution, 90 degrees C and 50 min, respectively. A good linearity within the range of 0.8-10 nM (r = 0.996) was attained, with a high detectability up to 0.5 nM. Analyses of Cu2+ ion in drinking water, lake water, seawater and biological samples were carried out and the method performances were found to agree well with that obtained by ICP-MS. The developed simple colorimetric sensor proved applicable for Cu2+ ion determination in real samples with high sensitivity and selectivity. (C) 2014 Elsevier B.V. All rights reserved.
Subtype期刊论文
Indexed BySCI
Language英语
WOS KeywordFUNCTIONALIZED GOLD NANOPARTICLES ; COPPER IONS ; ELECTROCHEMICAL DETERMINATION ; FLUORESCENT-PROBE ; AQUEOUS-SOLUTION ; AMINO-ACIDS ; SEAWATER ; KINETICS ; IRON ; DERIVATIZATION
WOS Research AreaInnovation Projects of the Chinese Academy of Sciences [KZCX2-EW-206] ; National Natural Science Foundation of China [31200041, 21205131, 21275158] ; Scientific Research Foundation for the Returned Overseas Chinese Scholars ; State Education Ministry ; Key Research Program of the Chinese Academy of Sciences [KZZD-EW-14]
WOS IDWOS:000345059100014
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8654
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorZhang, WW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. wwzhang@yic.ac.cn; lxchen@yic.ac.cn
Recommended Citation
GB/T 7714
Yin, Kun,Li, Bowei,Wang, Xiaochun,et al. Ultrasensitive colorimetric detection of Cu2+ ion based on catalytic oxidation of L-cysteine[J]. BIOSENSORS & BIOELECTRONICS,2015,64:81-87.
APA Yin, Kun.,Li, Bowei.,Wang, Xiaochun.,Zhang, Weiwei.,Chen, Lingxin.,...&lxchen@yic.ac.cn.(2015).Ultrasensitive colorimetric detection of Cu2+ ion based on catalytic oxidation of L-cysteine.BIOSENSORS & BIOELECTRONICS,64,81-87.
MLA Yin, Kun,et al."Ultrasensitive colorimetric detection of Cu2+ ion based on catalytic oxidation of L-cysteine".BIOSENSORS & BIOELECTRONICS 64(2015):81-87.
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