YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil
Ma, Ying; Oliveira, Rui S.; Nai, Fengjiao; Rajkumar, Mani; Luo, Yongming; Rocha, Ines; Freitas, Helena; Ma, Y (reprint author), Univ Coimbra, Dept Life Sci, Ctr Funct Ecol, P-3000456 Coimbra, Portugal. cathymaying@gmail.com
2015-06-01
Source PublicationJOURNAL OF ENVIRONMENTAL MANAGEMENT
ISSN0301-4797
Volume156Pages:62-69
Contribution Rank[Ma, Ying; Oliveira, Rui S.; Rocha, Ines; Freitas, Helena] Univ Coimbra, Dept Life Sci, Ctr Funct Ecol, P-3000456 Coimbra, Portugal; [Ma, Ying] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Oliveira, Rui S.] Univ Catolica Portuguesa, Escola Super Biotecnol, Lab Associado, CBQF Ctr Biotecnol Quim Fina, P-4202401 Oporto, Portugal; [Oliveira, Rui S.] Polytech Inst Porto, Sch Allied Hlth Sci, Res Ctr Hlth & Environm, P-4400330 Vila Nova De Gaia, Portugal; [Nai, Fengjiao] Guizhou Univ, Soil Sci Agr Coll, Guiyang 550025, Peoples R China; [Rajkumar, Mani] Cent Univ Tamil Nadu, Dept Life Sci, Collectorate Annexe, Thiruvarur 610004, Tamil Nadu, India; [Luo, Yongming] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
AbstractEndophyte-assisted phytoremediation has recently been suggested as a successful approach for ecological restoration of metal contaminated soils, however little information is available on the influence of endophytic bacteria on the phytoextraction capacity of metal hyperaccumulating plants in multimetal polluted soils. The aims of our study were to isolate and characterize metal-resistant and 1-aminocyclopropane-1-carboxylate (ACC) utilizing endophytic bacteria from tissues of the newly discovered Zn/Cd hyperaccumulator Sedum plumbizinci cola and to examine if these endophytic bacterial strains could improve the efficiency of phytoextraction of multi-metal contaminated soils. Among a collection of 42 metal resistant bacterial strains isolated from the tissues of S. plumbizinci cola grown on Pb/Zn mine tailings, five plant growth promoting endophytic bacterial strains (PGPE) were selected due to their ability to promote plant growth and to utilize ACC as the sole nitrogen source. The five isolates were identified as Bacillus pumilus E2S2, Bacillus sp. E1S2, Bacillus sp. E4S1, Achromobacter sp. E4L5 and Stenotrophomonas sp. E1L and subsequent testing revealed that they all exhibited traits associated with plant growth promotion, such as production of indole-3-acetic acid and siderophores and solubilization of phosphorus. These five strains showed high resistance to heavy metals (Cd, Zn and Pb) and various antibiotics. Further, inoculation of these ACC utilizing strains significantly increased the concentrations of water ektractable Cd and Zn in soil. Moreover, a pot experiment was conducted to elucidate the effects of inoculating metal-resistant ACC utilizing strains on the growth of S. plumbizincicola and its uptake of Cd, Zn and Pb in multi-metal contaminated soils. Out of the five strains, B. pumilus E2S2 significantly increased root (146%) and shoot (17%) length, fresh (37%) and dry biomass (32%) of S. plumbizincicola as well as plant Cd uptake (43%), whereas Bacillus sp. E1S2 significantly enhanced the accumulation of Zn (18%) in plants compared with non-inoculated controls. The inoculated strains also showed high levels of colonization in rhizosphere and plant tissues. Results demonstrate the potential to improve phytoextraction of soils contaminated with multiple heavy metals by inoculating metal hyperaccumulating plants with their own selected functional endophytic bacterial strains. (C) 2015 Elsevier Ltd. All rights reserved.; Endophyte-assisted phytoremediation has recently been suggested as a successful approach for ecological restoration of metal contaminated soils, however little information is available on the influence of endophytic bacteria on the phytoextraction capacity of metal hyperaccumulating plants in multimetal polluted soils. The aims of our study were to isolate and characterize metal-resistant and 1-aminocyclopropane-1-carboxylate (ACC) utilizing endophytic bacteria from tissues of the newly discovered Zn/Cd hyperaccumulator Sedum plumbizinci cola and to examine if these endophytic bacterial strains could improve the efficiency of phytoextraction of multi-metal contaminated soils. Among a collection of 42 metal resistant bacterial strains isolated from the tissues of S. plumbizinci cola grown on Pb/Zn mine tailings, five plant growth promoting endophytic bacterial strains (PGPE) were selected due to their ability to promote plant growth and to utilize ACC as the sole nitrogen source. The five isolates were identified as Bacillus pumilus E2S2, Bacillus sp. E1S2, Bacillus sp. E4S1, Achromobacter sp. E4L5 and Stenotrophomonas sp. E1L and subsequent testing revealed that they all exhibited traits associated with plant growth promotion, such as production of indole-3-acetic acid and siderophores and solubilization of phosphorus. These five strains showed high resistance to heavy metals (Cd, Zn and Pb) and various antibiotics. Further, inoculation of these ACC utilizing strains significantly increased the concentrations of water ektractable Cd and Zn in soil. Moreover, a pot experiment was conducted to elucidate the effects of inoculating metal-resistant ACC utilizing strains on the growth of S. plumbizincicola and its uptake of Cd, Zn and Pb in multi-metal contaminated soils. Out of the five strains, B. pumilus E2S2 significantly increased root (146%) and shoot (17%) length, fresh (37%) and dry biomass (32%) of S. plumbizincicola as well as plant Cd uptake (43%), whereas Bacillus sp. E1S2 significantly enhanced the accumulation of Zn (18%) in plants compared with non-inoculated controls. The inoculated strains also showed high levels of colonization in rhizosphere and plant tissues. Results demonstrate the potential to improve phytoextraction of soils contaminated with multiple heavy metals by inoculating metal hyperaccumulating plants with their own selected functional endophytic bacterial strains. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordEndophytic Bacteria Heavy Metals Hyperaccumulator Phytoextraction Sedum Plumbizincicola
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaEnvironmental Sciences
DOI10.1016/j.jenvman.2015.03.024
Funding OrganizationEnvironmental Sciences & Ecology
Indexed BySCI
WOS KeywordPLANT-GROWTH ; ACID ; CD ; CAERULESCENS ; INOCULATION
SubtypeArticle
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS IDWOS:000355036700008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8608
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorMa, Y (reprint author), Univ Coimbra, Dept Life Sci, Ctr Funct Ecol, P-3000456 Coimbra, Portugal. cathymaying@gmail.com
Recommended Citation
GB/T 7714
Ma, Ying,Oliveira, Rui S.,Nai, Fengjiao,et al. The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil[J]. JOURNAL OF ENVIRONMENTAL MANAGEMENT,2015,156:62-69.
APA Ma, Ying.,Oliveira, Rui S..,Nai, Fengjiao.,Rajkumar, Mani.,Luo, Yongming.,...&Ma, Y .(2015).The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil.JOURNAL OF ENVIRONMENTAL MANAGEMENT,156,62-69.
MLA Ma, Ying,et al."The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil".JOURNAL OF ENVIRONMENTAL MANAGEMENT 156(2015):62-69.
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