| Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na+/H+ Antiporter Gene from the Halophyte Kosteletzkya virginica | |
| Wang, Hongyan1,2,3; Tang, Xiaoli1,3; Shao, Chuyang4; Shao, Hongbo1,5; Wang, Honglei2; Shao, HB (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China. shaohongbochu@126.com | |
| 发表期刊 | SCIENTIFIC WORLD JOURNAL
 |
| ISSN | 1537-744X |
| 2014 | |
| 页码 | 141675 |
| 关键词 | Salt Tolerance Arabidopsis-thaliana Transport Salinity Plants Overexpression Expression |
| DOI | 10.1155/2014/141675 |
| 产权排序 | [Wang, Hongyan; Tang, Xiaoli; Shao, Hongbo] Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China; [Wang, Hongyan; Wang, Honglei] China Agr Univ, Yantai Acad, Yantai 264670, Peoples R China; [Wang, Hongyan; Tang, Xiaoli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Shao, Chuyang] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; [Shao, Hongbo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | A new plasma membrane Na+/H+ antiporter gene (named as KvSOS1) was cloned from the halophyte Kosteletzkya virginica by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology, which is a homologue of SOS1 (salt overly sensitive 1). The full-length cDNA is 3850 bp and contains an open reading frame (ORF) encoding a protein of 1147 amino acids with a molecular weight of 127.56 kDa and a theoretical pI of 6.18. Bioinformatics analysis indicated that the deduced protein appears to be a transmembrane protein with 12 transmembrane domains at the N-terminal region and a long hydrophilic tail in cytoplasm at its C-terminal region and shares 72-82% identity at the peptide level with other plant plasma membrane Na+/H+ antiporters.; A new plasma membrane Na+/H+ antiporter gene (named as KvSOS1) was cloned from the halophyte Kosteletzkya virginica by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology, which is a homologue of SOS1 (salt overly sensitive 1). The full-length cDNA is 3850 bp and contains an open reading frame (ORF) encoding a protein of 1147 amino acids with a molecular weight of 127.56 kDa and a theoretical pI of 6.18. Bioinformatics analysis indicated that the deduced protein appears to be a transmembrane protein with 12 transmembrane domains at the N-terminal region and a long hydrophilic tail in cytoplasm at its C-terminal region and shares 72-82% identity at the peptide level with other plant plasma membrane Na+/H+ antiporters. |
| 文章类型 | Article |
| 资助机构 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SALT TOLERANCE ; ARABIDOPSIS-THALIANA ; TRANSPORT ; SALINITY ; PLANTS ; OVEREXPRESSION ; EXPRESSION |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000343438500001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8571 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Shao, HB (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China. shaohongbochu@126.com |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China 2.China Agr Univ, Yantai Acad, Yantai 264670, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China 5.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hongyan,Tang, Xiaoli,Shao, Chuyang,et al. Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na+/H+ Antiporter Gene from the Halophyte Kosteletzkya virginica[J]. SCIENTIFIC WORLD JOURNAL,2014:141675. |
| APA | Wang, Hongyan,Tang, Xiaoli,Shao, Chuyang,Shao, Hongbo,Wang, Honglei,&Shao, HB .(2014).Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na+/H+ Antiporter Gene from the Halophyte Kosteletzkya virginica.SCIENTIFIC WORLD JOURNAL,141675. |
| MLA | Wang, Hongyan,et al."Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na+/H+ Antiporter Gene from the Halophyte Kosteletzkya virginica".SCIENTIFIC WORLD JOURNAL (2014):141675. |
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| Molecular Cloning an（2541KB） | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |||
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