Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene
Wang, L.1,2; Du, Y. C.1; Meng, X. F.1; Long, X. H.1; Liu, Z. P.1; Shao, H.2,3; Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn; shaohongbochu@126.com
发表期刊PLANT BIOSYSTEMS
ISSN1126-3504
2014-01-02
卷号148期号:1页码:133-139
关键词Bioethanol Jerusalem Artichoke Inulinase Saccharomyces Cerevisiae Gene Engineering
DOI10.1080/11263504.2013.856961
产权排序[Wang, L.; Du, Y. C.; Meng, X. F.; Long, X. H.; Liu, Z. P.] Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Wang, L.; Shao, H.] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Shao, H.] Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China
作者部门海岸带生物学与生物资源利用所重点实验室
英文摘要Jerusalem artichoke (Helianthus tuberosus L.), an important crop, containing over 50% inulin in its tubers on a dry weight basis is an agricultural and industrial crop with a great potential for production of ethanol and industrial products. Inulin is a good substrate for bioethanol production. Saccharomyces cerevisiae 6525 can produce high concentrations of ethanol, but it cannot synthesize inulinase. In this study, a new integration vector carrying inuA1 gene encoding exoinulinase was constructed and transformed into 18SrDNA site of industrial strain S. cerevisiae 6525. The obtained transformant, BR8, produced 1.1UmL(-1) inulinase activity within 72h and the dry cell weight reached 12.3gL(-1) within 48h. In a small-scale fermentation, BR8 produced 9.5% (v/v) ethanol, with a productivity rate of 0.385g ethanol per gram inulin, while wild-type S. cerevisiae 6525 produced only 3.3% (v/v) ethanol in the same conditions. In a 5-L fermentation, BR8 produced 14.0% (v/v) ethanol in fermentation medium containing inulin and 1% (w/v) (NH4)(2)SO4. The engineered S. cerevisiae 6525 carrying inuA1 converted pure nonhydrolyzed inulin directly into high concentrations of ethanol.; Jerusalem artichoke (Helianthus tuberosus L.), an important crop, containing over 50% inulin in its tubers on a dry weight basis is an agricultural and industrial crop with a great potential for production of ethanol and industrial products. Inulin is a good substrate for bioethanol production. Saccharomyces cerevisiae 6525 can produce high concentrations of ethanol, but it cannot synthesize inulinase. In this study, a new integration vector carrying inuA1 gene encoding exoinulinase was constructed and transformed into 18SrDNA site of industrial strain S. cerevisiae 6525. The obtained transformant, BR8, produced 1.1UmL(-1) inulinase activity within 72h and the dry cell weight reached 12.3gL(-1) within 48h. In a small-scale fermentation, BR8 produced 9.5% (v/v) ethanol, with a productivity rate of 0.385g ethanol per gram inulin, while wild-type S. cerevisiae 6525 produced only 3.3% (v/v) ethanol in the same conditions. In a 5-L fermentation, BR8 produced 14.0% (v/v) ethanol in fermentation medium containing inulin and 1% (w/v) (NH4)(2)SO4. The engineered S. cerevisiae 6525 carrying inuA1 converted pure nonhydrolyzed inulin directly into high concentrations of ethanol.
文章类型Article
资助机构Plant Sciences
收录类别SCI
语种英语
关键词[WOS]ETHANOL-PRODUCTION ; GROWTH ; EXPRESSION ; TOLERANCE ; TUBERS ; CHINA
研究领域[WOS]Plant Sciences
WOS记录号WOS:000333880000019
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/8564
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中国科学院烟台海岸带研究所
通讯作者Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn; shaohongbochu@126.com
作者单位1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China
3.Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China
推荐引用方式
GB/T 7714
Wang, L.,Du, Y. C.,Meng, X. F.,et al. Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene[J]. PLANT BIOSYSTEMS,2014,148(1):133-139.
APA Wang, L..,Du, Y. C..,Meng, X. F..,Long, X. H..,Liu, Z. P..,...&shaohongbochu@126.com.(2014).Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene.PLANT BIOSYSTEMS,148(1),133-139.
MLA Wang, L.,et al."Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene".PLANT BIOSYSTEMS 148.1(2014):133-139.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Direct production of(120KB) 开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, L.]的文章
[Du, Y. C.]的文章
[Meng, X. F.]的文章
百度学术
百度学术中相似的文章
[Wang, L.]的文章
[Du, Y. C.]的文章
[Meng, X. F.]的文章
必应学术
必应学术中相似的文章
[Wang, L.]的文章
[Du, Y. C.]的文章
[Meng, X. F.]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Direct production of bioethanol from Jerusalem artichoke inulin by gene-engineering Saccharomyces cerevisiae 6525 with exoinulinase gene.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。