| SSU rDNA Sequence Diversity and Seasonally Differentiated Distribution of Nanoplanktonic Ciliates in Neritic Bohai and Yellow Seas as Revealed by T-RFLP | |
Dong, Jun1,2; Shi, Fei2; Li, Han2; Zhang, Xiaoming3; Hu, Xiaozhong1; Gong, Jun2,3 ; Gong, J (reprint author), Chinese Acad Sci, Lab Microbial Ecol & Matter Cycles, Yantai Inst Coastal Zone Res, Yantai, Peoples R China. jgong@yic.ac.cn
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| 发表期刊 | PLOS ONE
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| ISSN | 1932-6203 |
| 2014-07-15 | |
| 卷号 | 9期号:7页码:e102640 |
| 关键词 | Operational Taxonomic Units Ribosomal-rna Genes Spatial-distribution Longitudinal Trends Protistan Diversity Maximum-likelihood Mediterranean Sea Annual Cycle Baltic Sea Marine |
| DOI | 10.1371/journal.pone.0102640 |
| 产权排序 | [Dong, Jun; Hu, Xiaozhong] Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao, Peoples R China; [Dong, Jun; Shi, Fei; Li, Han; Gong, Jun] Chinese Acad Sci, Lab Microbial Ecol & Matter Cycles, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Zhang, Xiaoming; Gong, Jun] S China Normal Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Nanociliates have been frequently found to be important players in the marine microbial loop, however, little is known about their diversity and distribution in coastal ecosystems. We investigated the molecular diversity and distribution patterns of nanoplanktonic oligotrich and choreotrich (OC) ciliates in surface water of three neritic basins of northern China, the South Yellow Sea (SYS), North Yellow Sea (NYS), and Bohai Sea (BS) in June and November 2011. SSU rRNA gene clone libraries generated from three summertime samples (sites B38, B4 and H8) were analyzed and revealed a large novel ribotype diversity, of which many were low-abundant phylotypes belonging to the subclass Oligotrichia, but divergent from described morphospecies. Based on the data of terminal-restriction fragment length polymorphism (T-RFLP) analysis of all 35 samples, we found that the T-RF richness was generally higher in the SYS than in the BS, and negatively correlated with the molar ratio of P to Si. Overall, multidimensional scaling and permutational multivariate analysis of variance of the community turnover demonstrated a distinct seasonal pattern but no basin-to-basin differentiation across all samples. Nevertheless, significant community differences among basins were recognized in the winter dataset. Mantel tests showed that the environmental factors, P: Si ratio, water temperature and concentration of dissolved oxygen (DO), determined the community across all samples. However, both biogeographic distance and environment shaped the community in winter, with DO being the most important physicochemical factor. Our results indicate that the stoichiometric ratio of P: Si is a key factor, through which the phytoplankton community may be shaped, resulting in a cascade effect on the diversity and community composition of OC nanociliates in the N-rich, Si-limited coastal surface waters, and that the Yellow Sea Warm Current drives the nanociliate community, and possibly the microbial food webs, in the coastal ecosystem in winter.; Nanociliates have been frequently found to be important players in the marine microbial loop, however, little is known about their diversity and distribution in coastal ecosystems. We investigated the molecular diversity and distribution patterns of nanoplanktonic oligotrich and choreotrich (OC) ciliates in surface water of three neritic basins of northern China, the South Yellow Sea (SYS), North Yellow Sea (NYS), and Bohai Sea (BS) in June and November 2011. SSU rRNA gene clone libraries generated from three summertime samples (sites B38, B4 and H8) were analyzed and revealed a large novel ribotype diversity, of which many were low-abundant phylotypes belonging to the subclass Oligotrichia, but divergent from described morphospecies. Based on the data of terminal-restriction fragment length polymorphism (T-RFLP) analysis of all 35 samples, we found that the T-RF richness was generally higher in the SYS than in the BS, and negatively correlated with the molar ratio of P to Si. Overall, multidimensional scaling and permutational multivariate analysis of variance of the community turnover demonstrated a distinct seasonal pattern but no basin-to-basin differentiation across all samples. Nevertheless, significant community differences among basins were recognized in the winter dataset. Mantel tests showed that the environmental factors, P: Si ratio, water temperature and concentration of dissolved oxygen (DO), determined the community across all samples. However, both biogeographic distance and environment shaped the community in winter, with DO being the most important physicochemical factor. Our results indicate that the stoichiometric ratio of P: Si is a key factor, through which the phytoplankton community may be shaped, resulting in a cascade effect on the diversity and community composition of OC nanociliates in the N-rich, Si-limited coastal surface waters, and that the Yellow Sea Warm Current drives the nanociliate community, and possibly the microbial food webs, in the coastal ecosystem in winter. |
| 文章类型 | Article |
| 资助机构 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | OPERATIONAL TAXONOMIC UNITS ; RIBOSOMAL-RNA GENES ; SPATIAL-DISTRIBUTION ; LONGITUDINAL TRENDS ; PROTISTAN DIVERSITY ; MAXIMUM-LIKELIHOOD ; MEDITERRANEAN SEA ; ANNUAL CYCLE ; BALTIC SEA ; MARINE |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000339992400097 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8542 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Gong, J (reprint author), Chinese Acad Sci, Lab Microbial Ecol & Matter Cycles, Yantai Inst Coastal Zone Res, Yantai, Peoples R China. jgong@yic.ac.cn |
| 作者单位 | 1.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao, Peoples R China 2.Chinese Acad Sci, Lab Microbial Ecol & Matter Cycles, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 3.S China Normal Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Jun,Shi, Fei,Li, Han,et al. SSU rDNA Sequence Diversity and Seasonally Differentiated Distribution of Nanoplanktonic Ciliates in Neritic Bohai and Yellow Seas as Revealed by T-RFLP[J]. PLOS ONE,2014,9(7):e102640. |
| APA | Dong, Jun.,Shi, Fei.,Li, Han.,Zhang, Xiaoming.,Hu, Xiaozhong.,...&Gong, J .(2014).SSU rDNA Sequence Diversity and Seasonally Differentiated Distribution of Nanoplanktonic Ciliates in Neritic Bohai and Yellow Seas as Revealed by T-RFLP.PLOS ONE,9(7),e102640. |
| MLA | Dong, Jun,et al."SSU rDNA Sequence Diversity and Seasonally Differentiated Distribution of Nanoplanktonic Ciliates in Neritic Bohai and Yellow Seas as Revealed by T-RFLP".PLOS ONE 9.7(2014):e102640. |
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| SSU rDNA Sequence Di（859KB） | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |||
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