Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri

doi:10.1128/AEM.00895-14

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	Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri
	Rotaru, Amelia-Elena; Shrestha, Pravin Malla; Liu, Fanghua; Markovaite, Beatrice; Chen, Shanshan; Nevin, Kelly P.; Lovley, Derek R.; Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@biology.sdu.dk
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
	2014-08
卷号	80 期号:15 页码:4599-4605
关键词	Sulfur-containing-compounds Fe(Iii) Oxide Reduction Anaerobic-digestion Pelobacter-carbinolicus Vertical-distribution Archaeal Community Syntrophic Growth Formate Transfer Defined Medium Paddy Soils
DOI	10.1128/AEM.00895-14
产权排序	[Rotaru, Amelia-Elena; Shrestha, Pravin Malla; Liu, Fanghua; Markovaite, Beatrice; Chen, Shanshan; Nevin, Kelly P.; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA; [Liu, Fanghua] Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Chen, Shanshan] Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangzhou 510275, Guangdong, Peoples R China
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	Direct interspecies electron transfer (DIET) is potentially an effective form of syntrophy in methanogenic communities, but little is known about the diversity of methanogens capable of DIET. The ability of Methanosarcina barkeri to participate in DIET was evaluated in coculture with Geobacter metallireducens. Cocultures formed aggregates that shared electrons via DIET during the stoichiometric conversion of ethanol to methane. Cocultures could not be initiated with a pilin-deficient G. metallireducens strain, suggesting that long-range electron transfer along pili was important for DIET. Amendments of granular activated carbon permitted the pilin-deficient G. metallireducens isolates to share electrons with M. barkeri, demonstrating that this conductive material could substitute for pili in promoting DIET. When M. barkeri was grown in coculture with the H-2-producing Pelobacter carbinolicus, incapable of DIET, M. barkeri utilized H-2 as an electron donor but metabolized little of the acetate that P. carbinolicus produced. This suggested that H-2, but not electrons derived from DIET, inhibited acetate metabolism. P. carbinolicus-M. barkeri cocultures did not aggregate, demonstrating that, unlike DIET, close physical contact was not necessary for interspecies H-2 transfer. M. barkeri is the second methanogen found to accept electrons via DIET and the first methanogen known to be capable of using either H-2 or electrons derived from DIET for CO2 reduction. Furthermore, M. barkeri is genetically tractable, making it a model organism for elucidating mechanisms by which methanogens make biological electrical connections with other cells.
文章类型	期刊论文
资助机构	Biotechnology & Applied Microbiology ; Microbiology
收录类别	SCI
语种	英语
关键词[WOS]	SULFUR-CONTAINING-COMPOUNDS ; FE(III) OXIDE REDUCTION ; ANAEROBIC-DIGESTION ; PELOBACTER-CARBINOLICUS ; VERTICAL-DISTRIBUTION ; ARCHAEAL COMMUNITY ; SYNTROPHIC GROWTH ; FORMATE TRANSFER ; DEFINED MEDIUM ; PADDY SOILS
研究领域[WOS]	Office of Science, U.S. Department of Energy [DE-SC0004485]
WOS记录号	WOS:000338707800014
引用统计	被引频次：598[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/8541
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@biology.sdu.dk
推荐引用方式 GB/T 7714	Rotaru, Amelia-Elena,Shrestha, Pravin Malla,Liu, Fanghua,et al. Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2014,80(15):4599-4605.
APA	Rotaru, Amelia-Elena.,Shrestha, Pravin Malla.,Liu, Fanghua.,Markovaite, Beatrice.,Chen, Shanshan.,...&Rotaru, AE .(2014).Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,80(15),4599-4605.
MLA	Rotaru, Amelia-Elena,et al."Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.15(2014):4599-4605.

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