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Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri
Rotaru, Amelia-Elena; Shrestha, Pravin Malla; Liu, Fanghua; Markovaite, Beatrice; Chen, Shanshan; Nevin, Kelly P.; Lovley, Derek R.; Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@biology.sdu.dk
2014-08
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume80Issue:15Pages:4599-4605
Contribution Rank[Rotaru, Amelia-Elena; Shrestha, Pravin Malla; Liu, Fanghua; Markovaite, Beatrice; Chen, Shanshan; Nevin, Kelly P.; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA; [Liu, Fanghua] Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Chen, Shanshan] Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangzhou 510275, Guangdong, Peoples R China
AbstractDirect interspecies electron transfer (DIET) is potentially an effective form of syntrophy in methanogenic communities, but little is known about the diversity of methanogens capable of DIET. The ability of Methanosarcina barkeri to participate in DIET was evaluated in coculture with Geobacter metallireducens. Cocultures formed aggregates that shared electrons via DIET during the stoichiometric conversion of ethanol to methane. Cocultures could not be initiated with a pilin-deficient G. metallireducens strain, suggesting that long-range electron transfer along pili was important for DIET. Amendments of granular activated carbon permitted the pilin-deficient G. metallireducens isolates to share electrons with M. barkeri, demonstrating that this conductive material could substitute for pili in promoting DIET. When M. barkeri was grown in coculture with the H-2-producing Pelobacter carbinolicus, incapable of DIET, M. barkeri utilized H-2 as an electron donor but metabolized little of the acetate that P. carbinolicus produced. This suggested that H-2, but not electrons derived from DIET, inhibited acetate metabolism. P. carbinolicus-M. barkeri cocultures did not aggregate, demonstrating that, unlike DIET, close physical contact was not necessary for interspecies H-2 transfer. M. barkeri is the second methanogen found to accept electrons via DIET and the first methanogen known to be capable of using either H-2 or electrons derived from DIET for CO2 reduction. Furthermore, M. barkeri is genetically tractable, making it a model organism for elucidating mechanisms by which methanogens make biological electrical connections with other cells.
KeywordSulfur-containing-compounds Fe(Iii) Oxide Reduction Anaerobic-digestion Pelobacter-carbinolicus Vertical-distribution Archaeal Community Syntrophic Growth Formate Transfer Defined Medium Paddy Soils
Department海岸带生物学与生物资源利用所重点实验室
Subject AreaBiotechnology & Applied Microbiology ; Microbiology
DOI10.1128/AEM.00895-14
Funding OrganizationBiotechnology & Applied Microbiology ; Microbiology
Indexed BySCI
WOS KeywordSULFUR-CONTAINING-COMPOUNDS ; FE(III) OXIDE REDUCTION ; ANAEROBIC-DIGESTION ; PELOBACTER-CARBINOLICUS ; VERTICAL-DISTRIBUTION ; ARCHAEAL COMMUNITY ; SYNTROPHIC GROWTH ; FORMATE TRANSFER ; DEFINED MEDIUM ; PADDY SOILS
Subtype期刊论文
Language英语
WOS Research AreaOffice of Science, U.S. Department of Energy [DE-SC0004485]
WOS IDWOS:000338707800014
Citation statistics
Cited Times:287[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8541
Collection海岸带生物学与生物资源保护实验室
Corresponding AuthorRotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@biology.sdu.dk
Recommended Citation
GB/T 7714
Rotaru, Amelia-Elena,Shrestha, Pravin Malla,Liu, Fanghua,et al. Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2014,80(15):4599-4605.
APA Rotaru, Amelia-Elena.,Shrestha, Pravin Malla.,Liu, Fanghua.,Markovaite, Beatrice.,Chen, Shanshan.,...&Rotaru, AE .(2014).Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,80(15),4599-4605.
MLA Rotaru, Amelia-Elena,et al."Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.15(2014):4599-4605.
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