| Contrasting spatiotemporal patterns and environmental drivers of diversity and community structure of ammonia oxidizers, denitrifiers, and anammox bacteria in sediments of estuarine tidal flats | |
Yang, Anjing; Zhang, Xiaoli ; Agogue, Helene; Dupuy, Christine; Gong, Jun; Zhang, XL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc, Lab Microbial Ecol & Matter Cycles, Yantai, Peoples R China. xlzhang@yic.ac.cn; jgong@yic.ac.cn
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| 发表期刊 | ANNALS OF MICROBIOLOGY
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| ISSN | 1590-4261 |
| 2015-06-01 | |
| 卷号 | 65期号:2页码:879-890 |
| 关键词 | Biogeography Community Turnover Environmental Factors Heavy Metals Nitrogen Cycle |
| DOI | 10.1007/s13213-014-0929-5 |
| 产权排序 | [Yang, Anjing; Zhang, Xiaoli; Gong, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc, Lab Microbial Ecol & Matter Cycles, Yantai, Peoples R China; [Yang, Anjing] Univ Chinese Acad Sci, Beijing, Peoples R China; [Agogue, Helene; Dupuy, Christine] Univ La Rochelle, CNRS, Littoral Environm & Soc LIENSS, UMR 7266, La Rochelle, France |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | The spatial and temporal patterns of diversity, community structure, and their drivers are fundamental issues in microbial ecology. This study aimed to investigate the relative importance of spatial and seasonal controls on the distribution of nitrogen cycling microbes in sediments of estuarine tidal flats, and to test the hypothesis that metals impact the distribution of nitrogen-cycling microbes in the coastal system. Two layers of sediment samples were collected from three estuarine tidal flats of Laizhou Bay in 2010 winter and 2011 summer. The alpha diversities (Shannon and Simpson indices) and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA), denitrifier and anammox bacteria (AMB) were revealed using denaturing gradient gel electrophoresis and clone library analysis of amoA, nosZ and 16S rRNA gene markers. We found that both AOB and AMB exhibited distinct seasonal patterns in either alpha diversity or community turnover; AOA had different alpha diversities in two layers, but neither spatial nor seasonal patterns were found for their community turnover. However, no distinct spatiotemporal pattern was observed for either diversity or community composition of nosZ-type denitrifiers. For correlations between alpha diversities and environmental factors, significant correlations were found between AOB and ammonium, temperature and As, between denitrifiers and nitrite, salinity and Pb, and between AMB and Pb, ratio of organic carbon to nitrogen, ammonium, pH and dissolved oxygen. Salinity and sediment grain size were the most important factors shaping AOB and AOA communities, respectively; whereas AMB community structure was mostly determined by temperature, dissolved oxygen, pH and heavy metals As and Cd. These results stress that ammonia oxidizers, denitrifiers and anammox bacteria have generally different distributional patterns across time and space, and heavy metals might have contributed to their differentiated distributions in coastal sediments.; The spatial and temporal patterns of diversity, community structure, and their drivers are fundamental issues in microbial ecology. This study aimed to investigate the relative importance of spatial and seasonal controls on the distribution of nitrogen cycling microbes in sediments of estuarine tidal flats, and to test the hypothesis that metals impact the distribution of nitrogen-cycling microbes in the coastal system. Two layers of sediment samples were collected from three estuarine tidal flats of Laizhou Bay in 2010 winter and 2011 summer. The alpha diversities (Shannon and Simpson indices) and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA), denitrifier and anammox bacteria (AMB) were revealed using denaturing gradient gel electrophoresis and clone library analysis of amoA, nosZ and 16S rRNA gene markers. We found that both AOB and AMB exhibited distinct seasonal patterns in either alpha diversity or community turnover; AOA had different alpha diversities in two layers, but neither spatial nor seasonal patterns were found for their community turnover. However, no distinct spatiotemporal pattern was observed for either diversity or community composition of nosZ-type denitrifiers. For correlations between alpha diversities and environmental factors, significant correlations were found between AOB and ammonium, temperature and As, between denitrifiers and nitrite, salinity and Pb, and between AMB and Pb, ratio of organic carbon to nitrogen, ammonium, pH and dissolved oxygen. Salinity and sediment grain size were the most important factors shaping AOB and AOA communities, respectively; whereas AMB community structure was mostly determined by temperature, dissolved oxygen, pH and heavy metals As and Cd. These results stress that ammonia oxidizers, denitrifiers and anammox bacteria have generally different distributional patterns across time and space, and heavy metals might have contributed to their differentiated distributions in coastal sediments. |
| 文章类型 | Article |
| 资助机构 | Biotechnology & Applied Microbiology ; Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | 16S RIBOSOMAL-RNA ; FRANCISCO BAY ESTUARY ; HEAVY-METALS ; SPATIAL-DISTRIBUTION ; MOLECULAR-DETECTION ; RELATIVE ABUNDANCE ; MARINE-SEDIMENTS ; RIVER ESTUARY ; JIAOZHOU BAY ; WASTE-WATER |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000354724300029 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8500 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Zhang, XL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc, Lab Microbial Ecol & Matter Cycles, Yantai, Peoples R China. xlzhang@yic.ac.cn; jgong@yic.ac.cn |
| 推荐引用方式 GB/T 7714 | Yang, Anjing,Zhang, Xiaoli,Agogue, Helene,et al. Contrasting spatiotemporal patterns and environmental drivers of diversity and community structure of ammonia oxidizers, denitrifiers, and anammox bacteria in sediments of estuarine tidal flats[J]. ANNALS OF MICROBIOLOGY,2015,65(2):879-890. |
| APA | Yang, Anjing.,Zhang, Xiaoli.,Agogue, Helene.,Dupuy, Christine.,Gong, Jun.,...&jgong@yic.ac.cn.(2015).Contrasting spatiotemporal patterns and environmental drivers of diversity and community structure of ammonia oxidizers, denitrifiers, and anammox bacteria in sediments of estuarine tidal flats.ANNALS OF MICROBIOLOGY,65(2),879-890. |
| MLA | Yang, Anjing,et al."Contrasting spatiotemporal patterns and environmental drivers of diversity and community structure of ammonia oxidizers, denitrifiers, and anammox bacteria in sediments of estuarine tidal flats".ANNALS OF MICROBIOLOGY 65.2(2015):879-890. |
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