基于构建微生物传感器的甲基对硫磷降解菌的分离鉴定及其降解特性研究

	基于构建微生物传感器的甲基对硫磷降解菌的分离鉴定及其降解特性研究
其他题名	Isolation and Characterization of Methyl Parathion-degrading Bacteria Based on Microbial Sensor Construction
	耿芳芳; 王慧; 秦伟; 丁保君
发表期刊	农业资源与环境学报
ISSN	1005-4944
	2014
卷号	31 期号:6 页码:521-526
关键词	甲基对硫磷克雷伯氏菌微生物降解微生物传感器
产权排序	大连理工大学化工与环境生命学部;中国科学院烟台海岸带研究所;
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	有机磷农药是目前环境中残留量最多的农药之一,对其残留量的检测及降解机制的研究对于环境污染及生态修复具有重要意义。微生物传感器由生物学元件与换能器构成,因具有成本低廉、易于微型化及选择性高等特点而被广泛应用于各种生化物质的分析和检测。本文从长期受农药污染的土壤中分离出4株能以甲基对硫磷为碳源生长的菌株,根据形态特征和16S r RNA基因序列同源性分析,对4株降解菌进行鉴定,利用高效液相色谱测定降解率,选取降解率最高的1株菌进行降解机制研究,以期将其应用于测定环境中甲基对硫磷残留的电位型微生物传感器的构建。结果表明,在甲基对硫磷初始浓度50 mg·L-1、30℃、p H 7.0的培养条件下培养7...
中文摘要	Methyl parathion（MP）, a kind of typical organophosphates pesticides（OPs）, is widely used as agricultural insecticides. However, due to their neurotoxic effects on humans, the elimination of OPs has become increasingly important. Microbial sensors are consisted of biological components and transducers. Owing to their attractive advantages including low cost, easy of miniaturization and excellent selectivity, they have been widely used for environmental analysis. In this paper, four novel bacterial strains capable of utilizing methyl parathion as the sole carbon source were isolated from pesticide contaminated soils. These four isolates were identified based on morphological characteristics and 16S rRNA gene sequences analysis, and their capability of degrading methyl parathion were investigated by high performance liquid chromatography. The highest degrading efficiency strain was selected for further study of degrading mechanism. The results indicated that degradation rate of these four strains were all over 78%after incubation at 30 ℃, pH 7.0 for 7 d with the original concentration of methyl parathion 50 mg·L^-1. The highest degradation rate was up to 100%. 16S rRNA gene sequences indicated that strain MP-6 was affiliated into the genus klebsiella. The LC-MS results indicated that methyl parathion was hydrolyzed to dimethyl thiophosphoric acid and p-nitrophenol by MP-6. A little of p-nitrophenol molecules could be further metabolized to 4-nitrocatechol and 1, 2, 4-benzenetrio. The results indicated that based on detecting the potential signal of intermediate product p-nitrophenol, the strain MP-6 could be used to construct microbial sensors for determination of organophosphorus pesticides in environment.
文章类型	期刊论文
资助机构	国家自然科学青年基金(21107008)
收录类别	其他
语种	中文
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/8429
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
推荐引用方式 GB/T 7714	耿芳芳,王慧,秦伟,等. 基于构建微生物传感器的甲基对硫磷降解菌的分离鉴定及其降解特性研究[J]. 农业资源与环境学报,2014,31(6):521-526.
APA	耿芳芳,王慧,秦伟,&丁保君.(2014).基于构建微生物传感器的甲基对硫磷降解菌的分离鉴定及其降解特性研究.农业资源与环境学报,31(6),521-526.
MLA	耿芳芳,et al."基于构建微生物传感器的甲基对硫磷降解菌的分离鉴定及其降解特性研究".农业资源与环境学报 31.6(2014):521-526.

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