The invention discloses a method for preparing bioethanol by using enteromorpha as a raw material, comprising the following steps : (1) rinsing the enteromorpha; (2) grinding the enteromorpha until thegrain size is 0.5-2mm; (3) putting the enteromorpha obtained in the step (2) into an acidolysis device in a material-liquid ratio of 1 : 20-1 : 5, adding acid until the acid concentration is 0.5-2.5% (w/w) and hydrolyzing the mixture in a reaction kettle at a temperature of 110-150 DEG C; (4) adding calcium hydroxide into enteromorpha hydrolysate obtained in the step (3) to neutralize the hydrolysate, standing at room temperature and removing solid matters by press filtering to obtain hydrolysate rich in glucose and xylose; (5) inoculating the hydrolysate obtained in the step (4) into 5% of candida shehatae to be fermented at a temperature of 30 DEG C for 1-5d; (6) distilling fermentation liquor to obtain the bioethanol. The method uses the enteromorpha as the raw material for ethanol fermentation, combines the characteristics of the enteromorpha and dispenses with enzymolysis. The hydrolysate can be directly used for bioethanol fermentation.
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