Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation
Yang, Dinglong1,2; Wei, Xiumei1; Yang, Jianmin1; Yang, Jialong3; Xu, Jie1,2; Fang, Jinghui1; Wang, Sheng1,2; Liu, Xiangquan1; Wei, XM (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. xiumeiwei@hotmail.com; lxq6808@163.com
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2013-10-01
卷号35期号:4页码:1325-1328
关键词Solen Grandis Litaf Innate Immunity Transcription Factor Real-time Pcr
产权排序[Yang, Dinglong; Wei, Xiumei; Yang, Jianmin; Xu, Jie; Fang, Jinghui; Wang, Sheng; Liu, Xiangquan] Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China; [Yang, Dinglong; Xu, Jie; Wang, Sheng] Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China; [Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
英文摘要Lipopolysaccharide-induced TNF-alpha factor (LITAF) is one of the most important transcription factors mediating TNF-alpha transcription. In the present study, a LITAF gene (designated as SgLITAF) was identified from razor clams Solen grandis. The full-length cDNA of SgLITAF was of 1476 bp, encoding a polypeptide of 130 amino acids showed high similarity to other known LITAFs. SgLITAF encoded a LITAF domain and the Zn2+-binding motifs in the domain were well conserved. The mRNA transcripts of SgLITAF were detected in all tested tissues of healthy razor clams, including mantle, gill, gonad, hemocytes, muscle and hepatopancreas, and with the highest expression level in hepatopancreas. The expression level of SgLITAF in hemocytes was significantly up-regulated (P < 0.01) after razor clams were stimulated by LPS or beta-1, 3-glucan, but no obvious fluctuation of SgLITAF mRNA expression was observed after PGN stimulation. All the results indicated that there might be a LITAF-regulated TNF-alpha signaling pathway existing in S. grandis, which involved in the immune response not only against gram-negative bacteria but also towards fungi. (C) 2013 Elsevier Ltd. All rights reserved.; Lipopolysaccharide-induced TNF-alpha factor (LITAF) is one of the most important transcription factors mediating TNF-alpha transcription. In the present study, a LITAF gene (designated as SgLITAF) was identified from razor clams Solen grandis. The full-length cDNA of SgLITAF was of 1476 bp, encoding a polypeptide of 130 amino acids showed high similarity to other known LITAFs. SgLITAF encoded a LITAF domain and the Zn2+-binding motifs in the domain were well conserved. The mRNA transcripts of SgLITAF were detected in all tested tissues of healthy razor clams, including mantle, gill, gonad, hemocytes, muscle and hepatopancreas, and with the highest expression level in hepatopancreas. The expression level of SgLITAF in hemocytes was significantly up-regulated (P < 0.01) after razor clams were stimulated by LPS or beta-1, 3-glucan, but no obvious fluctuation of SgLITAF mRNA expression was observed after PGN stimulation. All the results indicated that there might be a LITAF-regulated TNF-alpha signaling pathway existing in S. grandis, which involved in the immune response not only against gram-negative bacteria but also towards fungi. (C) 2013 Elsevier Ltd. All rights reserved.
文章类型Article
资助机构NSFC [31202025]; Program of Agriculture Thoroughbred Project, Shandong Province, China; Taishan Scholar Position Funding of Aquatic Animal Nutrition and Feed
收录类别SCI
语种英语
关键词[WOS]MOLECULAR-CLONING ; HOMOLOG ; GENE
研究领域[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000324849800031
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7064
专题中国科学院烟台海岸带研究所知识产出
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Wei, XM (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. xiumeiwei@hotmail.com; lxq6808@163.com
作者单位1.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China
2.Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714
Yang, Dinglong,Wei, Xiumei,Yang, Jianmin,et al. Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(4):1325-1328.
APA Yang, Dinglong.,Wei, Xiumei.,Yang, Jianmin.,Yang, Jialong.,Xu, Jie.,...&lxq6808@163.com.(2013).Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation.FISH & SHELLFISH IMMUNOLOGY,35(4),1325-1328.
MLA Yang, Dinglong,et al."Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation".FISH & SHELLFISH IMMUNOLOGY 35.4(2013):1325-1328.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Identification of a (474KB) 开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Dinglong]的文章
[Wei, Xiumei]的文章
[Yang, Jianmin]的文章
百度学术
百度学术中相似的文章
[Yang, Dinglong]的文章
[Wei, Xiumei]的文章
[Yang, Jianmin]的文章
必应学术
必应学术中相似的文章
[Yang, Dinglong]的文章
[Wei, Xiumei]的文章
[Yang, Jianmin]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。