Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes
Sun Guohua1; Liu Xiangquan1; Ren Lihua1; Yang Jianmin1; Wei Xiumei1; Yang Jialong2; Liu, XQ (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. lxq6808@163.com
发表期刊JOURNAL OF OCEAN UNIVERSITY OF CHINA
ISSN1672-5182
2014-02-01
卷号13期号:1页码:169-173
关键词Solen Grandis Cdna Library Est Sequence Defense And Immune Functional Gene
产权排序[Sun Guohua; Liu Xiangquan; Ren Lihua; Yang Jianmin; Wei Xiumei] Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China; [Yang Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
英文摘要The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the 'switching mechanism at the 5'-end of the RNA transcript' (SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu mu L-1 and a storage capacity of 1.05x10(6) cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes (E-value < 1e (-5); percent identity > 80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities.; The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the 'switching mechanism at the 5'-end of the RNA transcript' (SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu mu L-1 and a storage capacity of 1.05x10(6) cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes (E-value < 1e (-5); percent identity > 80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities.
文章类型Article
资助机构Shandong Program of Agriculture Thoroughbred Project; Marine Special Research Foundation of China [200805031]; Taishan Scholar position funding of Aquatic Animal Nutrition and Feed; NSFC [31202025]
收录类别SCI
语种英语
关键词[WOS]OYSTER CRASSOSTREA-GIGAS ; HEAT-SHOCK PROTEINS ; PACIFIC OYSTER ; HSP70 GENES ; EXPRESSION ; HEAT-SHOCK-PROTEIN-70 ; EVOLUTION ; APOPTOSIS ; DISEASE ; FAMILY
研究领域[WOS]Oceanography
WOS记录号WOS:000330312000022
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7059
专题中国科学院烟台海岸带研究所知识产出
通讯作者Liu, XQ (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. lxq6808@163.com
作者单位1.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
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Sun Guohua,Liu Xiangquan,Ren Lihua,et al. Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2014,13(1):169-173.
APA Sun Guohua.,Liu Xiangquan.,Ren Lihua.,Yang Jianmin.,Wei Xiumei.,...&Liu, XQ .(2014).Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes.JOURNAL OF OCEAN UNIVERSITY OF CHINA,13(1),169-173.
MLA Sun Guohua,et al."Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes".JOURNAL OF OCEAN UNIVERSITY OF CHINA 13.1(2014):169-173.
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