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题名:
Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment
作者: Yang, Shaoli1; Liu, Shengchen2; Ren, Zongming1; Jiao, Xudong1; Qin, Song1
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN号: 1532-0456
出版日期: 2014-03-01
卷号: 160, 页码:60-65
关键词: Apoptosis ; Fatty acid beta-oxidation ; Oxidative stress ; Perfluorononanoic acid ; Zebrafish
学科分类: Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
产权排序: [Yang, Shaoli; Ren, Zongming; Jiao, Xudong; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Shengchen] Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China
通讯作者: Yang, SL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. slyang@yic.ac.cn
中文摘要: Perfluorononanoic acid (PFNA) is an organic pollutant ubiquitous in the environment. However, the potential toxicity of PFNA remains largely unknown in teleost fish. This study defined the oxidative stress and related transcriptional effects of PFNA at various concentrations on zebrafish larvae. Activities of superoxide dismutase were induced in PFNA-treated groups but attenuated with exposure to higher concentration. Catalase activity and lipid peroxidation were significantly inhibited or increased at the highest concentration, respectively. To test the apoptotic pathway, several genes related to cell apoptosis were examined using real-time PCR. The expression of p53, apoptosis-inducing factor (AIF) and c-Jun NH (2)-terminal kinase (JNK) was partially increased, while Bcl-2, an anti-apoptotic gene, was reduced, with no significant effects on Bax and caspase-3 during the exposure period. The effect of PFNA on lipid beta-oxidation system was investigated by examining the activity of peroxisome fatty acyl-COA oxidase (ACOX) and the expression of peroxisome proliferating activating receptors (PPARs). ACOX activity was moderately elevated with marginal significance and was not a significant consequence of PPAR alpha and PPAR gamma expression. The overall results suggest that turbulence of oxidative stress and apoptotic pathway is involved in PFNA-induced toxicity in zebrafish larvae, and the gene expression patterns are able to reveal some potential mechanisms of developmental toxicity. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
英文摘要: Perfluorononanoic acid (PFNA) is an organic pollutant ubiquitous in the environment. However, the potential toxicity of PFNA remains largely unknown in teleost fish. This study defined the oxidative stress and related transcriptional effects of PFNA at various concentrations on zebrafish larvae. Activities of superoxide dismutase were induced in PFNA-treated groups but attenuated with exposure to higher concentration. Catalase activity and lipid peroxidation were significantly inhibited or increased at the highest concentration, respectively. To test the apoptotic pathway, several genes related to cell apoptosis were examined using real-time PCR. The expression of p53, apoptosis-inducing factor (AIF) and c-Jun NH (2)-terminal kinase (JNK) was partially increased, while Bcl-2, an anti-apoptotic gene, was reduced, with no significant effects on Bax and caspase-3 during the exposure period. The effect of PFNA on lipid beta-oxidation system was investigated by examining the activity of peroxisome fatty acyl-COA oxidase (ACOX) and the expression of peroxisome proliferating activating receptors (PPARs). ACOX activity was moderately elevated with marginal significance and was not a significant consequence of PPAR alpha and PPAR gamma expression. The overall results suggest that turbulence of oxidative stress and apoptotic pathway is involved in PFNA-induced toxicity in zebrafish larvae, and the gene expression patterns are able to reveal some potential mechanisms of developmental toxicity. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
研究领域[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
关键词[WOS]: PERFLUOROOCTANE SULFONATE PFOS ; CARBON-CHAIN LENGTH ; ZEBRAFISH EMBRYOS ; GENE-EXPRESSION ; PERFLUORODODECANOIC ACID ; DEVELOPMENTAL TOXICITY ; LIPID-PEROXIDATION ; PROTEOMIC ANALYSIS ; REACTIVE OXYGEN ; APOPTOSIS
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000330917400008
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内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/7012
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China

Recommended Citation:
Yang, Shaoli,Liu, Shengchen,Ren, Zongming,et al. Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2014,160:60-65.
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