Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment
Yang, Shaoli1; Liu, Shengchen2; Ren, Zongming1; Jiao, Xudong1; Qin, Song1; Yang, SL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. slyang@yic.ac.cn
2014-03-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN1532-0456
卷号160页码:60-65
产权排序[Yang, Shaoli; Ren, Zongming; Jiao, Xudong; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Shengchen] Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China
摘要Perfluorononanoic acid (PFNA) is an organic pollutant ubiquitous in the environment. However, the potential toxicity of PFNA remains largely unknown in teleost fish. This study defined the oxidative stress and related transcriptional effects of PFNA at various concentrations on zebrafish larvae. Activities of superoxide dismutase were induced in PFNA-treated groups but attenuated with exposure to higher concentration. Catalase activity and lipid peroxidation were significantly inhibited or increased at the highest concentration, respectively. To test the apoptotic pathway, several genes related to cell apoptosis were examined using real-time PCR. The expression of p53, apoptosis-inducing factor (AIF) and c-Jun NH (2)-terminal kinase (JNK) was partially increased, while Bcl-2, an anti-apoptotic gene, was reduced, with no significant effects on Bax and caspase-3 during the exposure period. The effect of PFNA on lipid beta-oxidation system was investigated by examining the activity of peroxisome fatty acyl-COA oxidase (ACOX) and the expression of peroxisome proliferating activating receptors (PPARs). ACOX activity was moderately elevated with marginal significance and was not a significant consequence of PPAR alpha and PPAR gamma expression. The overall results suggest that turbulence of oxidative stress and apoptotic pathway is involved in PFNA-induced toxicity in zebrafish larvae, and the gene expression patterns are able to reveal some potential mechanisms of developmental toxicity. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.; Perfluorononanoic acid (PFNA) is an organic pollutant ubiquitous in the environment. However, the potential toxicity of PFNA remains largely unknown in teleost fish. This study defined the oxidative stress and related transcriptional effects of PFNA at various concentrations on zebrafish larvae. Activities of superoxide dismutase were induced in PFNA-treated groups but attenuated with exposure to higher concentration. Catalase activity and lipid peroxidation were significantly inhibited or increased at the highest concentration, respectively. To test the apoptotic pathway, several genes related to cell apoptosis were examined using real-time PCR. The expression of p53, apoptosis-inducing factor (AIF) and c-Jun NH (2)-terminal kinase (JNK) was partially increased, while Bcl-2, an anti-apoptotic gene, was reduced, with no significant effects on Bax and caspase-3 during the exposure period. The effect of PFNA on lipid beta-oxidation system was investigated by examining the activity of peroxisome fatty acyl-COA oxidase (ACOX) and the expression of peroxisome proliferating activating receptors (PPARs). ACOX activity was moderately elevated with marginal significance and was not a significant consequence of PPAR alpha and PPAR gamma expression. The overall results suggest that turbulence of oxidative stress and apoptotic pathway is involved in PFNA-induced toxicity in zebrafish larvae, and the gene expression patterns are able to reveal some potential mechanisms of developmental toxicity. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
关键词Apoptosis Fatty Acid Beta-oxidation Oxidative Stress Perfluorononanoic Acid Zebrafish
学科领域Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
项目资助者National Natural Science Foundation of China [41001336]
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收录类别SCI
关键词[WOS]PERFLUOROOCTANE SULFONATE PFOS ; CARBON-CHAIN LENGTH ; ZEBRAFISH EMBRYOS ; GENE-EXPRESSION ; PERFLUORODODECANOIC ACID ; DEVELOPMENTAL TOXICITY ; LIPID-PEROXIDATION ; PROTEOMIC ANALYSIS ; REACTIVE OXYGEN ; APOPTOSIS
文章类型Article
语种英语
资助项目海岸带生物学与生物资源利用所重点实验室
WOS研究方向Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS记录号WOS:000330917400008
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7012
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Yang, SL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. slyang@yic.ac.cn
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Yang, Shaoli,Liu, Shengchen,Ren, Zongming,et al. Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2014,160:60-65.
APA Yang, Shaoli,Liu, Shengchen,Ren, Zongming,Jiao, Xudong,Qin, Song,&Yang, SL .(2014).Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,160,60-65.
MLA Yang, Shaoli,et al."Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 160(2014):60-65.
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