Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress
Liu Zhixiang1,2; Cui Hongli2; Liu Zhengyi1,2; Wang Yinchu2; Cui Yulin2; Liu Zhaopu1; Qin Song2; Liu, ZP (reprint author), Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
2014-03-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
卷号32期号:2页码:403-418
产权排序[Liu Zhixiang; Liu Zhengyi; Liu Zhaopu] Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China; [Liu Zhixiang; Cui Hongli; Liu Zhengyi; Wang Yinchu; Cui Yulin; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
摘要In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.; In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.
关键词Synechocystis Sp Pcc6803 Cyanobacteria Cdna Microarray Transcriptomics Low Temperature Stress
学科领域Limnology ; Oceanography
项目资助者National Natural Science Foundation of China [40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area; Public Science and Technology Research Funds Projects of the Ocean [200905021, 201205027]; Outstanding Young Scholars Fellowship of Shandong Province [JQ200914]; Natural Science Foundation of Shandong Province [ZR2012DQ015]; Guangdong Province Comprehensive Strategic Cooperation Project of the Chinese Academy of Sciences [2011A090100040]
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收录类别SCI
关键词[WOS]COLD-REGULATED GENES ; SP STRAIN PCC-6803 ; PROTEIN-KINASE ; CYANOBACTERIUM SYNECHOCYSTIS ; SIGNAL-TRANSDUCTION ; UNICELLULAR CYANOBACTERIUM ; MEMBRANE-FLUIDITY ; ARABIDOPSIS-THALIANA ; STRUCTURAL-ANALYSIS ; OMEGA-3 DESATURASE
文章类型Article
语种英语
资助项目海岸带生物学与生物资源利用所重点实验室
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS记录号WOS:000332739600018
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7010
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Liu, ZP (reprint author), Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
作者单位1.Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Liu Zhixiang,Cui Hongli,Liu Zhengyi,et al. Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):403-418.
APA Liu Zhixiang.,Cui Hongli.,Liu Zhengyi.,Wang Yinchu.,Cui Yulin.,...&sqin@yic.ac.cn.(2014).Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),403-418.
MLA Liu Zhixiang,et al."Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):403-418.
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