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题名:
Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress
作者: Liu Zhixiang1,2; Cui Hongli2; Liu Zhengyi1,2; Wang Yinchu2; Cui Yulin2; Liu Zhaopu1; Qin Song2
刊名: CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN号: 0254-4059
出版日期: 2014-03-01
卷号: 32, 期号:2, 页码:403-418
关键词: Synechocystis sp PCC6803 ; cyanobacteria ; cDNA microarray ; transcriptomics ; low temperature stress
学科分类: Limnology ; Oceanography
产权排序: [Liu Zhixiang; Liu Zhengyi; Liu Zhaopu] Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China; [Liu Zhixiang; Cui Hongli; Liu Zhengyi; Wang Yinchu; Cui Yulin; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者: Liu, ZP (reprint author), Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sea@njau.edu.cn ; sqin@yic.ac.cn
中文摘要: In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.
英文摘要: In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.
研究领域[WOS]: Marine & Freshwater Biology ; Oceanography
关键词[WOS]: COLD-REGULATED GENES ; SP STRAIN PCC-6803 ; PROTEIN-KINASE ; CYANOBACTERIUM SYNECHOCYSTIS ; SIGNAL-TRANSDUCTION ; UNICELLULAR CYANOBACTERIUM ; MEMBRANE-FLUIDITY ; ARABIDOPSIS-THALIANA ; STRUCTURAL-ANALYSIS ; OMEGA-3 DESATURASE
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000332739600018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/7010
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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作者单位: 1.Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China

Recommended Citation:
Liu Zhixiang,Cui Hongli,Liu Zhengyi,et al. Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):403-418.
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