Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress
Liu Zhixiang1,2; Cui Hongli2; Liu Zhengyi1,2; Wang Yinchu2; Cui Yulin2; Liu Zhaopu1; Qin Song2; Liu, ZP (reprint author), Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
2014-03-01
Volume32Issue:2Pages:403-418
KeywordSynechocystis Sp Pcc6803 Cyanobacteria Cdna Microarray Transcriptomics Low Temperature Stress
Contribution Rank[Liu Zhixiang; Liu Zhengyi; Liu Zhaopu] Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China; [Liu Zhixiang; Cui Hongli; Liu Zhengyi; Wang Yinchu; Cui Yulin; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
AbstractIn this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.; In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.
Funding OrganizationNational Natural Science Foundation of China [40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area; Public Science and Technology Research Funds Projects of the Ocean [200905021, 201205027]; Outstanding Young Scholars Fellowship of Shandong Province [JQ200914]; Natural Science Foundation of Shandong Province [ZR2012DQ015]; Guangdong Province Comprehensive Strategic Cooperation Project of the Chinese Academy of Sciences [2011A090100040]
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordCOLD-REGULATED GENES ; SP STRAIN PCC-6803 ; PROTEIN-KINASE ; CYANOBACTERIUM SYNECHOCYSTIS ; SIGNAL-TRANSDUCTION ; UNICELLULAR CYANOBACTERIUM ; MEMBRANE-FLUIDITY ; ARABIDOPSIS-THALIANA ; STRUCTURAL-ANALYSIS ; OMEGA-3 DESATURASE
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS IDWOS:000332739600018
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/7010
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorLiu, ZP (reprint author), Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
Affiliation1.Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
Recommended Citation
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Liu Zhixiang,Cui Hongli,Liu Zhengyi,et al. Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):403-418.
APA Liu Zhixiang.,Cui Hongli.,Liu Zhengyi.,Wang Yinchu.,Cui Yulin.,...&sqin@yic.ac.cn.(2014).Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),403-418.
MLA Liu Zhixiang,et al."Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):403-418.
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