Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses
Feng, Jianghua1; Li, Jinquan1; Wu, Huifeng2; Chen, Zhong1
发表期刊METABOLOMICS
ISSN1573-3882
2013-08-01
卷号9期号:4页码:874-886
关键词Silica Nanoparticles Metabolomic Analysis Nmr Spectroscopy Nanomedicine Hela Cell
产权排序[Feng, Jianghua; Li, Jinquan; Chen, Zhong] Xiamen Univ, Dept Elect Sci, Fujian Prov Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China; [Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
通讯作者Feng, JH (reprint author), Xiamen Univ, Dept Elect Sci, Fujian Prov Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China. jianghua.feng@xmu.edu.cn
英文摘要Silica nanoparticles are increasingly used in the biomedical fields due to their excellent solubility, high stability and favorable biocompatibility. However, despite being considered of low genotoxicity, their bio-related adverse effects have attracted particular concern from both the scientific field and the public. In this study, human cervical adenocarcinoma cells (HeLa line) were exposed to 0.01 or 1.0 mg/mL of hydrophilic silica nanoparticles. The H-1 NMR spectroscopy coupled with multivariate statistical analysis were used to characterize the metabolic variations of intracellular metabolites and the compositional changes of the corresponding culture media. At the early stage of silica nanoparticles-exposure, no obvious dose-effect of HeLa cell metabolome was observed, which implied that cellular stress-response regulated the metabolic variations of HeLa cell. Silica nanoparticles induced the increases of lipids including triglyceride, LDL, VLDL and lactate/alanine ratio and the decreases of alanine, ATP, choline, creatine, glycine, glycerol, isoleucine, leucine, phenylalanine, tyrosine, and valine, which involved in membrane modification, catabolism of carbohydrate and protein, and stress-response. Subsequently, a complicated synergistic effect of stress-response and toxicological-effect dominated the biochemical process and metabolic response, which was demonstrated in the reverse changes of some metabolites including acetate, ADP, ATP, choline, creatine, glutamine, glycine, lysine, methionine, phenylalanine and valine between 6 and 48 h post-treatment of silica nanoparticles. The toxicological-effects induced by high-dosage silica nanoparticles could be derived from the elevated levels of ATP and ADP, the utilization of glucose and amino acids and the production of metabolic end-products such as glutamate, glycine, lysine, methionine, phenylalanine, and valine. The results indicated that it is important and necessary to pursue further the physiological responses of silica nanoparticles in animal models and human before their practical use. NMR-based metabolomic analysis helps to understand the biological mechanisms of silica nanoparticles and their metabolic fate, and further, it offers an ideal platform for establishing the bio-safety of existing and new nanomaterials.; Silica nanoparticles are increasingly used in the biomedical fields due to their excellent solubility, high stability and favorable biocompatibility. However, despite being considered of low genotoxicity, their bio-related adverse effects have attracted particular concern from both the scientific field and the public. In this study, human cervical adenocarcinoma cells (HeLa line) were exposed to 0.01 or 1.0 mg/mL of hydrophilic silica nanoparticles. The H-1 NMR spectroscopy coupled with multivariate statistical analysis were used to characterize the metabolic variations of intracellular metabolites and the compositional changes of the corresponding culture media. At the early stage of silica nanoparticles-exposure, no obvious dose-effect of HeLa cell metabolome was observed, which implied that cellular stress-response regulated the metabolic variations of HeLa cell. Silica nanoparticles induced the increases of lipids including triglyceride, LDL, VLDL and lactate/alanine ratio and the decreases of alanine, ATP, choline, creatine, glycine, glycerol, isoleucine, leucine, phenylalanine, tyrosine, and valine, which involved in membrane modification, catabolism of carbohydrate and protein, and stress-response. Subsequently, a complicated synergistic effect of stress-response and toxicological-effect dominated the biochemical process and metabolic response, which was demonstrated in the reverse changes of some metabolites including acetate, ADP, ATP, choline, creatine, glutamine, glycine, lysine, methionine, phenylalanine and valine between 6 and 48 h post-treatment of silica nanoparticles. The toxicological-effects induced by high-dosage silica nanoparticles could be derived from the elevated levels of ATP and ADP, the utilization of glucose and amino acids and the production of metabolic end-products such as glutamate, glycine, lysine, methionine, phenylalanine, and valine. The results indicated that it is important and necessary to pursue further the physiological responses of silica nanoparticles in animal models and human before their practical use. NMR-based metabolomic analysis helps to understand the biological mechanisms of silica nanoparticles and their metabolic fate, and further, it offers an ideal platform for establishing the bio-safety of existing and new nanomaterials.
文章类型Article
资助机构National Natural Science Foundation of China [20605025, 21175099, 81272581]; Fundamental Research Funds for the Central Universities [2011121046]
收录类别SCI
语种英语
关键词[WOS]SPINNING H-1-NMR SPECTROSCOPY ; IN-VITRO ; DIOXIDE ; DELIVERY ; SIO2 ; MITOCHONDRIAL ; CONSEQUENCES ; CHEMOMETRICS ; EXPRESSION ; PULMONARY
研究领域[WOS]Endocrinology & Metabolism
WOS记录号WOS:000322019100013
引用统计
被引频次:74[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6967
专题中国科学院海岸带环境过程与生态修复重点实验室
作者单位1.Xiamen Univ, Dept Elect Sci, Fujian Prov Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
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GB/T 7714
Feng, Jianghua,Li, Jinquan,Wu, Huifeng,et al. Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses[J]. METABOLOMICS,2013,9(4):874-886.
APA Feng, Jianghua,Li, Jinquan,Wu, Huifeng,&Chen, Zhong.(2013).Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses.METABOLOMICS,9(4),874-886.
MLA Feng, Jianghua,et al."Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses".METABOLOMICS 9.4(2013):874-886.
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