YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge
Cong Ming1,2; Lu Jiasen3; Wu Huifeng1,2; Zhao Jianmin1,2
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
2013-09-01
Volume31Issue:5Pages:1002-1009
KeywordCadmium Listonella Anguillarum Crassostrea Gigas Defensive Enzyme Gene Expression
Contribution Rank[Cong Ming; Wu Huifeng; Zhao Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Cong Ming; Wu Huifeng; Zhao Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
AbstractHeavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium (Cd) on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), acid phosphatase (ACP), Na+, K+ -ATPase in gills and hepatopancreas, and phenoloxidase-like (POL) enzyme in hemolymph were assayed. In addition, the expression levels of several genes, including heat shock protein 90 (HSP90), metallothionein (MT), and bactericidal/permeability increasing (BPI) protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD, ACP, POL, and GPx in hepatopancreas, and the expression of HSP90 were down-regulated, whereas GPx activity in the gill, Na+, K+-ATPase activities in both tissues, and MT expression was increased in Cdexposed oysters post L. anguillarum challenge. However, BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters' immune responses and energy metabolism following vibrio infection.; Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium (Cd) on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), acid phosphatase (ACP), Na+, K+ -ATPase in gills and hepatopancreas, and phenoloxidase-like (POL) enzyme in hemolymph were assayed. In addition, the expression levels of several genes, including heat shock protein 90 (HSP90), metallothionein (MT), and bactericidal/permeability increasing (BPI) protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD, ACP, POL, and GPx in hepatopancreas, and the expression of HSP90 were down-regulated, whereas GPx activity in the gill, Na+, K+-ATPase activities in both tissues, and MT expression was increased in Cdexposed oysters post L. anguillarum challenge. However, BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters' immune responses and energy metabolism following vibrio infection.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordMESSENGER-RNA ; FREE-RADICALS ; EXPOSURE ; EXPRESSION ; IMMUNE ; HEMOCYTES ; VIRGINICA ; STRESS ; GENE ; METABOLISM
WOS Research AreaMarine & Freshwater Biology ; Oceanography
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS IDWOS:000324111300008
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6953
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
Recommended Citation
GB/T 7714
Cong Ming,Lu Jiasen,Wu Huifeng,et al. Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(5):1002-1009.
APA Cong Ming,Lu Jiasen,Wu Huifeng,&Zhao Jianmin.(2013).Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(5),1002-1009.
MLA Cong Ming,et al."Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.5(2013):1002-1009.
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