Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Highly Sensitive Surface-Enhanced Raman Scattering Sensing of Heparin Based on Antiaggregation of Functionalized Silver Nanoparticles | |
Wang, Xiaokun1,2; Chen, Ling1,3; Fu, Xiuli1,3; Chen, Lingxin1,2 ; Ding, Yanjun2
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| 发表期刊 | ACS APPLIED MATERIALS & INTERFACES
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| ISSN | 1944-8244 |
| 2013-11-13 | |
| 卷号 | 5期号:21页码:11059-11065 |
| 关键词 | Heparin Surface-enhanced Raman Scattering Silver Nanoparticles Antiaggregation |
| 产权排序 | [Wang, Xiaokun; Chen, Ling; Fu, Xiuli; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaokun; Chen, Lingxin; Ding, Yanjun] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China; [Chen, Ling; Fu, Xiuli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 英文摘要 | We report a simple and sensitive surface-enhanced Raman scattering (SERS) platform for the detection of heparin, based on antiaggregation of 4-mercaptopyridine (4-MPY) functionalized silver nanoparticles (Ag NPs). Here, protamine was employed as a medium for inducing the aggregation of negatively charged 4-MPY functionalized Ag NPs through surface electrostatic interaction, which resulted in significantly enhanced Raman signal of the Raman reporter. However, in the presence of heparin, the interaction between heparin and protamine decreased the concentration of free protamine, which dissipated the aggregated 4-MPY functionalized Ag NPs and thus decreased Raman enhancement effect. The degree of aggregation and Raman enhancement effect was proportional to the concentration of added heparin. Under optimized assay conditions, good linear relationship was obtained over the range of 0.5-150 ng/mL (R-2 = 0.998) with a minimum detectable concentration of 0.5 ng/mL in standard aqueous solution. Furthermore, the developed method was also successfully applied for detecting heparin in fetal bovine serum samples with a linear range of 1-400 ng/mL.; We report a simple and sensitive surface-enhanced Raman scattering (SERS) platform for the detection of heparin, based on antiaggregation of 4-mercaptopyridine (4-MPY) functionalized silver nanoparticles (Ag NPs). Here, protamine was employed as a medium for inducing the aggregation of negatively charged 4-MPY functionalized Ag NPs through surface electrostatic interaction, which resulted in significantly enhanced Raman signal of the Raman reporter. However, in the presence of heparin, the interaction between heparin and protamine decreased the concentration of free protamine, which dissipated the aggregated 4-MPY functionalized Ag NPs and thus decreased Raman enhancement effect. The degree of aggregation and Raman enhancement effect was proportional to the concentration of added heparin. Under optimized assay conditions, good linear relationship was obtained over the range of 0.5-150 ng/mL (R-2 = 0.998) with a minimum detectable concentration of 0.5 ng/mL in standard aqueous solution. Furthermore, the developed method was also successfully applied for detecting heparin in fetal bovine serum samples with a linear range of 1-400 ng/mL. |
| 文章类型 | Article |
| 资助机构 | Scientific Research Foundation for the Returned Overseas Chinese Scholars; State Education Ministry; National Natural Science Foundation of China [21275158]; Innovation Projects of the Chinese Academy of Sciences [KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ROOM-TEMPERATURE ; SPECTROSCOPY ; IONS ; NANOPROBES ; PARTICLES ; NANORODS ; SENSOR |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
| WOS记录号 | WOS:000327103500085 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6936 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaokun,Chen, Ling,Fu, Xiuli,et al. Highly Sensitive Surface-Enhanced Raman Scattering Sensing of Heparin Based on Antiaggregation of Functionalized Silver Nanoparticles[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(21):11059-11065. |
| APA | Wang, Xiaokun,Chen, Ling,Fu, Xiuli,Chen, Lingxin,&Ding, Yanjun.(2013).Highly Sensitive Surface-Enhanced Raman Scattering Sensing of Heparin Based on Antiaggregation of Functionalized Silver Nanoparticles.ACS APPLIED MATERIALS & INTERFACES,5(21),11059-11065. |
| MLA | Wang, Xiaokun,et al."Highly Sensitive Surface-Enhanced Raman Scattering Sensing of Heparin Based on Antiaggregation of Functionalized Silver Nanoparticles".ACS APPLIED MATERIALS & INTERFACES 5.21(2013):11059-11065. |
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