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Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge
Liu, Xiaoli1; Zhao, Jianmin2,3; Wu, Huifeng2,3; Wang, Qing2,3
2013-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume35Issue:6Pages:1969-1975
Contribution Rank[Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Zhao, Jianmin; Wu, Huifeng; Wang, Qing] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhao, Jianmin; Wu, Huifeng; Wang, Qing] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
AbstractManila clam Ruditapes philippinarum is an important marine aquaculture shellfish. This species has several pedigrees including White, Zebra, Liangdao Red and Marine Red distributing in the coastal areas in North China. In this work, we studied the metabolic differences induced by Vibrio harveyi in hepatopancreas from White and Zebra clams using NMR-based metabolomics. Metabolic responses (e.g., amino acids, glucose, glycogen, ATP and succinate) and altered mRNA expression levels of related genes (ATP synthase, heat shock protein 90, defensin and lysozyme) suggested that V harveyi induced clear disruption in energy metabolism and immune stresses in both White and Zebra clam hepatopancreas. However, V. harveyi caused obvious osmotic stress in Zebra clam hepatopancreas, which was not observed in V. harveyi-challenged White clams samples. In addition, V. harveyi challenge induced more severe disruption in energy metabolism and immune stress in White clams than in Zebra clams. Overall, our results indicated that the biological differences between different pedigrees of R. philippinarum should be considered in immunity studies. (C) 2013 Elsevier Ltd. All rights reserved.; Manila clam Ruditapes philippinarum is an important marine aquaculture shellfish. This species has several pedigrees including White, Zebra, Liangdao Red and Marine Red distributing in the coastal areas in North China. In this work, we studied the metabolic differences induced by Vibrio harveyi in hepatopancreas from White and Zebra clams using NMR-based metabolomics. Metabolic responses (e.g., amino acids, glucose, glycogen, ATP and succinate) and altered mRNA expression levels of related genes (ATP synthase, heat shock protein 90, defensin and lysozyme) suggested that V harveyi induced clear disruption in energy metabolism and immune stresses in both White and Zebra clam hepatopancreas. However, V. harveyi caused obvious osmotic stress in Zebra clam hepatopancreas, which was not observed in V. harveyi-challenged White clams samples. In addition, V. harveyi challenge induced more severe disruption in energy metabolism and immune stress in White clams than in Zebra clams. Overall, our results indicated that the biological differences between different pedigrees of R. philippinarum should be considered in immunity studies. (C) 2013 Elsevier Ltd. All rights reserved.
Corresponding AuthorLiu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylxl2009@gmail.com
KeywordMetabolomics Ruditapes Philippinarum Pedigree Vibrio Harveyi Hepatopancreas
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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Indexed BySCI
WOS KeywordNMR METABOLOMICS ; INVERTEBRATES ; VERTEBRATES ; CHAIN
SubtypeArticle
Language英语
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000330158800032
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6928
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xiaoli,Zhao, Jianmin,Wu, Huifeng,et al. Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(6):1969-1975.
APA Liu, Xiaoli,Zhao, Jianmin,Wu, Huifeng,&Wang, Qing.(2013).Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge.FISH & SHELLFISH IMMUNOLOGY,35(6),1969-1975.
MLA Liu, Xiaoli,et al."Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge".FISH & SHELLFISH IMMUNOLOGY 35.6(2013):1969-1975.
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