Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge
Liu, Xiaoli1; Zhao, Jianmin2,3; Wu, Huifeng2,3; Wang, Qing2,3
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2013-12-01
卷号35期号:6页码:1969-1975
关键词Metabolomics Ruditapes Philippinarum Pedigree Vibrio Harveyi Hepatopancreas
产权排序[Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Zhao, Jianmin; Wu, Huifeng; Wang, Qing] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhao, Jianmin; Wu, Huifeng; Wang, Qing] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
通讯作者Liu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylxl2009@gmail.com
英文摘要Manila clam Ruditapes philippinarum is an important marine aquaculture shellfish. This species has several pedigrees including White, Zebra, Liangdao Red and Marine Red distributing in the coastal areas in North China. In this work, we studied the metabolic differences induced by Vibrio harveyi in hepatopancreas from White and Zebra clams using NMR-based metabolomics. Metabolic responses (e.g., amino acids, glucose, glycogen, ATP and succinate) and altered mRNA expression levels of related genes (ATP synthase, heat shock protein 90, defensin and lysozyme) suggested that V harveyi induced clear disruption in energy metabolism and immune stresses in both White and Zebra clam hepatopancreas. However, V. harveyi caused obvious osmotic stress in Zebra clam hepatopancreas, which was not observed in V. harveyi-challenged White clams samples. In addition, V. harveyi challenge induced more severe disruption in energy metabolism and immune stress in White clams than in Zebra clams. Overall, our results indicated that the biological differences between different pedigrees of R. philippinarum should be considered in immunity studies. (C) 2013 Elsevier Ltd. All rights reserved.; Manila clam Ruditapes philippinarum is an important marine aquaculture shellfish. This species has several pedigrees including White, Zebra, Liangdao Red and Marine Red distributing in the coastal areas in North China. In this work, we studied the metabolic differences induced by Vibrio harveyi in hepatopancreas from White and Zebra clams using NMR-based metabolomics. Metabolic responses (e.g., amino acids, glucose, glycogen, ATP and succinate) and altered mRNA expression levels of related genes (ATP synthase, heat shock protein 90, defensin and lysozyme) suggested that V harveyi induced clear disruption in energy metabolism and immune stresses in both White and Zebra clam hepatopancreas. However, V. harveyi caused obvious osmotic stress in Zebra clam hepatopancreas, which was not observed in V. harveyi-challenged White clams samples. In addition, V. harveyi challenge induced more severe disruption in energy metabolism and immune stress in White clams than in Zebra clams. Overall, our results indicated that the biological differences between different pedigrees of R. philippinarum should be considered in immunity studies. (C) 2013 Elsevier Ltd. All rights reserved.
文章类型Article
收录类别SCI
语种英语
关键词[WOS]NMR METABOLOMICS ; INVERTEBRATES ; VERTEBRATES ; CHAIN
研究领域[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000330158800032
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6928
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中国科学院海岸带环境过程与生态修复重点实验室
作者单位1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
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Liu, Xiaoli,Zhao, Jianmin,Wu, Huifeng,et al. Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(6):1969-1975.
APA Liu, Xiaoli,Zhao, Jianmin,Wu, Huifeng,&Wang, Qing.(2013).Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge.FISH & SHELLFISH IMMUNOLOGY,35(6),1969-1975.
MLA Liu, Xiaoli,et al."Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge".FISH & SHELLFISH IMMUNOLOGY 35.6(2013):1969-1975.
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