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Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum
Alternative TitleSynthesis of TiO2 nanoparticles on mesoporous aluminosilicate Al-SBA-15 in supercritical CO2 for photocatalytic decolorization of methylene blue.pdf
Wu, Huifeng1; Ji, Chenglong1,2; Wei, Lei1,2; Zhao, Jianmin1; Lu, Hongjian3
2013-12-06
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume94Pages:54-67
Contribution Rank[Wu, Huifeng; Ji, Chenglong; Wei, Lei; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Ji, Chenglong; Wei, Lei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Lu, Hongjian] Nanjing Univ, Inst Chem & BioMed Sci ICBMS, Nanjing 210046, Jiangsu, Peoples R China
AbstractThe outbreak of pathogens can induce diseases and lead to massive mortalities of aquaculture animals including fish, mollusk and shrimp. In this work, the responses induced by Micrococcus luteus and Vibrio anguillarum were investigated in hepatopancreas of mussel Mytilus galloprovincialis using proteomics and metabolomics. Metabolic biomarkers demonstrated that M. luteus and V. anguillarum injections could induce osmotic stress and disturbance in energy metabolism. And the uniquely and more markedly altered metabolic biomarkers (glutamine, succinate, aspartate, glucose, ATP, homarine and tyrosine) indicated that V. anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. The differentially altered proteins meant that M. luteus and V. anguillarum induced different effects in mussels. However, the common proteomic biomarkers, arginine kinase and small heat shock protein, demonstrated that these two bacteria induced similar effects including oxidative stress and disturbance in energy metabolism in M. galloprovincialis. In addition, some metabolic biomarkers, ATP and glutamine, were confirmed by related proteins including arginine kinase, ATP synthase, nucleoside diphosphate kinase and glutamine synthetase in bacteria-challenged mussels. This study demonstrated that proteomics and metabolomics could provide an insightful view into the effects of environmental pathogens to the marine mussel M. galloprovincialis. Biological significance The outbreak of pathogens can lead to diseases and massive mortalities of aquaculture animals including fish, mollusk and shrimp. The mussel M. galloprovincialis distributes widely along the Bohai coast and is popularly consumed as delicious seafood by local residents. This bivalve has become one of the important species in marine aquaculture industry in China. Therefore a study on pathogen-induced effects is necessary. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the differential effects induced by the representative Gram-positive (M. luteus) and Gram-negative (V. anguillarum) bacteria in M. galloprovincialis. (C) 2013 Elsevier B.V. All rights reserved.; The outbreak of pathogens can induce diseases and lead to massive mortalities of aquaculture animals including fish, mollusk and shrimp. In this work, the responses induced by Micrococcus luteus and Vibrio anguillarum were investigated in hepatopancreas of mussel Mytilus galloprovincialis using proteomics and metabolomics. Metabolic biomarkers demonstrated that M. luteus and V. anguillarum injections could induce osmotic stress and disturbance in energy metabolism. And the uniquely and more markedly altered metabolic biomarkers (glutamine, succinate, aspartate, glucose, ATP, homarine and tyrosine) indicated that V. anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. The differentially altered proteins meant that M. luteus and V. anguillarum induced different effects in mussels. However, the common proteomic biomarkers, arginine kinase and small heat shock protein, demonstrated that these two bacteria induced similar effects including oxidative stress and disturbance in energy metabolism in M. galloprovincialis. In addition, some metabolic biomarkers, ATP and glutamine, were confirmed by related proteins including arginine kinase, ATP synthase, nucleoside diphosphate kinase and glutamine synthetase in bacteria-challenged mussels. This study demonstrated that proteomics and metabolomics could provide an insightful view into the effects of environmental pathogens to the marine mussel M. galloprovincialis.
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
KeywordProteomics Metabolomics Mytilus Galloprovincialis Bacteria
Subject AreaBiochemical Research Methods
DOI10.1016/j.jprot.2013.09.001
Funding OrganizationChinese Academy of Sciences [KZZD-EW-14-03]
URL查看原文
Indexed BySCI
WOS KeywordLASER-DESORPTION/IONIZATION-TIME ; NUCLEAR-MAGNETIC-RESONANCE ; EARTHWORM EISENIA-FOETIDA ; SCALLOP CHLAMYS-FARRERI ; GENE-EXPRESSION ; MARINE BIVALVES ; MEDITERRANEAN MUSSEL ; NMR METABOLOMICS ; IMMUNE-RESPONSE ; PROTEIN
SubtypeArticle
Language英语
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS Research AreaBiochemistry & Molecular Biology
WOS IDWOS:000330493400004
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6926
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Nanjing Univ, Inst Chem & BioMed Sci ICBMS, Nanjing 210046, Jiangsu, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Huifeng,Ji, Chenglong,Wei, Lei,et al. Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum[J]. JOURNAL OF PROTEOMICS,2013,94:54-67.
APA Wu, Huifeng,Ji, Chenglong,Wei, Lei,Zhao, Jianmin,&Lu, Hongjian.(2013).Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum.JOURNAL OF PROTEOMICS,94,54-67.
MLA Wu, Huifeng,et al."Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum".JOURNAL OF PROTEOMICS 94(2013):54-67.
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