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题名:
Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers
作者: Cai, Xiaoqiang1,2,3; Jinhua LI(李金花)2,3; Zhang, Zhong2,3,5; Wang, Gang2,3; Song, Xingliang4; You, Jinmao1; Chen, Lingxin1,2,3
刊名: TALANTA
ISSN号: 0039-9140
出版日期: 2014-03-01
卷号: 120, 页码:297-303
关键词: Molecularly imprinted polymer ; Fluorescence detection ; L-cysteine ; Chemodosimeter ; Rhodamine B
学科分类: Chemistry, Analytical
产权排序: [Cai, Xiaoqiang; You, Jinmao; Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China; [Cai, Xiaoqiang; Li, Jinhua; Zhang, Zhong; Wang, Gang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Cai, Xiaoqiang; Li, Jinhua; Zhang, Zhong; Wang, Gang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; [Song, Xingliang] Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China; [Zhang, Zhong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者: Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
中文摘要: A chemodosimeter-based fluorescent detection method coupled with molecularly imprinted polymers (MIPs) extraction was developed for determination of L-cysteine (L-Cys) by combining molecular imprinting technique with fluorescent chemodosimeter. The MIPs prepared by precipitation polymerization with L-Cys as template, possessed high specific surface area of 145 m(2)/g and good thermal stability without decomposition lower than 300 degrees C, and were successfully applied as an adsorbent with excellent selectivity for L-Cys over other amino acids, and enantioselectivity was also demonstrated. A novel chemodosimeter, rhodamine B1, was synthesized for discriminating L-Cys from its structurally similar homocysteine and glutathione as well as various possibly co-existing biospecies in aqueous solutions with notable fluorescence enhancement when adding L-Cys. As L-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the L-Cys could be sensed optically. High detectability up to 12.5 nM was obtained. An excellent linearity was found within the wide range of 0.05-50 mu M (r=0.9996), and reasonable relative standard deviations ranging from 0.3% to 3.5% were attained. Such typical features as high selectivity, high sensitivity, easy operation and low cost enabled this MIPs-fluorometry to be potentially applicable for routine detection of trace L-Cys. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要: A chemodosimeter-based fluorescent detection method coupled with molecularly imprinted polymers (MIPs) extraction was developed for determination of L-cysteine (L-Cys) by combining molecular imprinting technique with fluorescent chemodosimeter. The MIPs prepared by precipitation polymerization with L-Cys as template, possessed high specific surface area of 145 m(2)/g and good thermal stability without decomposition lower than 300 degrees C, and were successfully applied as an adsorbent with excellent selectivity for L-Cys over other amino acids, and enantioselectivity was also demonstrated. A novel chemodosimeter, rhodamine B1, was synthesized for discriminating L-Cys from its structurally similar homocysteine and glutathione as well as various possibly co-existing biospecies in aqueous solutions with notable fluorescence enhancement when adding L-Cys. As L-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the L-Cys could be sensed optically. High detectability up to 12.5 nM was obtained. An excellent linearity was found within the wide range of 0.05-50 mu M (r=0.9996), and reasonable relative standard deviations ranging from 0.3% to 3.5% were attained. Such typical features as high selectivity, high sensitivity, easy operation and low cost enabled this MIPs-fluorometry to be potentially applicable for routine detection of trace L-Cys. (C) 2013 Elsevier B.V. All rights reserved.
研究领域[WOS]: Chemistry
关键词[WOS]: COLORIMETRIC SENSOR ; GOLD NANOPARTICLES ; AMINO-ACIDS ; RECOGNITION ; ELECTRODE ; DERIVATIZATION ; CHROMATOGRAPHY ; HOMOCYSTEINE ; PLASMA
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000331917700040
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/6900
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
4.Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Cai, Xiaoqiang,Li, Jinhua,Zhang, Zhong,et al. Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers[J]. TALANTA,2014,120:297-303.
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