Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers
Cai, Xiaoqiang1,2,3; Li, Jinhua2,3; Zhang, Zhong2,3,5; Wang, Gang2,3; Song, Xingliang4; You, Jinmao1; Chen, Lingxin1,2,3
2014-03-01
发表期刊TALANTA
ISSN0039-9140
卷号120页码:297-303
产权排序[Cai, Xiaoqiang; You, Jinmao; Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China; [Cai, Xiaoqiang; Li, Jinhua; Zhang, Zhong; Wang, Gang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Cai, Xiaoqiang; Li, Jinhua; Zhang, Zhong; Wang, Gang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; [Song, Xingliang] Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China; [Zhang, Zhong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
摘要A chemodosimeter-based fluorescent detection method coupled with molecularly imprinted polymers (MIPs) extraction was developed for determination of L-cysteine (L-Cys) by combining molecular imprinting technique with fluorescent chemodosimeter. The MIPs prepared by precipitation polymerization with L-Cys as template, possessed high specific surface area of 145 m(2)/g and good thermal stability without decomposition lower than 300 degrees C, and were successfully applied as an adsorbent with excellent selectivity for L-Cys over other amino acids, and enantioselectivity was also demonstrated. A novel chemodosimeter, rhodamine B1, was synthesized for discriminating L-Cys from its structurally similar homocysteine and glutathione as well as various possibly co-existing biospecies in aqueous solutions with notable fluorescence enhancement when adding L-Cys. As L-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the L-Cys could be sensed optically. High detectability up to 12.5 nM was obtained. An excellent linearity was found within the wide range of 0.05-50 mu M (r=0.9996), and reasonable relative standard deviations ranging from 0.3% to 3.5% were attained. Such typical features as high selectivity, high sensitivity, easy operation and low cost enabled this MIPs-fluorometry to be potentially applicable for routine detection of trace L-Cys. (C) 2013 Elsevier B.V. All rights reserved.; A chemodosimeter-based fluorescent detection method coupled with molecularly imprinted polymers (MIPs) extraction was developed for determination of L-cysteine (L-Cys) by combining molecular imprinting technique with fluorescent chemodosimeter. The MIPs prepared by precipitation polymerization with L-Cys as template, possessed high specific surface area of 145 m(2)/g and good thermal stability without decomposition lower than 300 degrees C, and were successfully applied as an adsorbent with excellent selectivity for L-Cys over other amino acids, and enantioselectivity was also demonstrated. A novel chemodosimeter, rhodamine B1, was synthesized for discriminating L-Cys from its structurally similar homocysteine and glutathione as well as various possibly co-existing biospecies in aqueous solutions with notable fluorescence enhancement when adding L-Cys. As L-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the L-Cys could be sensed optically. High detectability up to 12.5 nM was obtained. An excellent linearity was found within the wide range of 0.05-50 mu M (r=0.9996), and reasonable relative standard deviations ranging from 0.3% to 3.5% were attained. Such typical features as high selectivity, high sensitivity, easy operation and low cost enabled this MIPs-fluorometry to be potentially applicable for routine detection of trace L-Cys. (C) 2013 Elsevier B.V. All rights reserved.
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
关键词Molecularly Imprinted Polymer Fluorescence Detection L-cysteine Chemodosimeter Rhodamine b
学科领域Chemistry, Analytical
项目资助者National Natural Science Foundation of China [21105117, 21275158, 21275068, 21275089]; Natural Science Foundation of Shandong Province of China [ZR2010BQ027]; Chinese Academy of Sciences
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收录类别SCI
关键词[WOS]COLORIMETRIC SENSOR ; GOLD NANOPARTICLES ; AMINO-ACIDS ; RECOGNITION ; ELECTRODE ; DERIVATIZATION ; CHROMATOGRAPHY ; HOMOCYSTEINE ; PLASMA
文章类型Article
语种英语
资助项目中国科学院海岸带环境过程与生态修复重点实验室
WOS研究方向Chemistry
WOS记录号WOS:000331917700040
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6900
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
4.Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Cai, Xiaoqiang,Li, Jinhua,Zhang, Zhong,et al. Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers[J]. TALANTA,2014,120:297-303.
APA Cai, Xiaoqiang.,Li, Jinhua.,Zhang, Zhong.,Wang, Gang.,Song, Xingliang.,...&Chen, Lingxin.(2014).Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers.TALANTA,120,297-303.
MLA Cai, Xiaoqiang,et al."Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers".TALANTA 120(2014):297-303.
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