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The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics
Ji Chenglong1,2,3; Wu Huifeng1,2; Liu Xiaoli1,2,3; Zhao Jianmin1,2; Yu Junbao1,2; Yin Xiuli1,2
2013-03-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume31Issue:2Pages:345-352
Contribution Rank[Ji Chenglong; Wu Huifeng; Liu Xiaoli; Zhao Jianmin; Yu Junbao; Yin Xiuli] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China; [Ji Chenglong; Wu Huifeng; Liu Xiaoli; Zhao Jianmin; Yu Junbao; Yin Xiuli] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ji Chenglong; Liu Xiaoli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
AbstractRuditapes philippinarum, a clam that thrives in intertidal zones of various salinities, is a useful biomonitor to marine contaminants. We investigated the influence of dilution to 75% and 50% of normal seawater salinity (31.1) on the responses of the digestive gland of R. philippinarum to arsenic exposure (20 mu g/L), using nuclear magnetic resonance (NMR)-based metabolomics. After acute arsenic exposure for 48 h, salinity-dependent differential metabolic responses were detected. In normal seawater, arsenic exposure increased the concentrations of branched-chain amino acids, and of threonine, proline, phosphocholine and adenosine, and it decreased the levels of alanine, hypotaurine, glucose, glycogen and ATP in the digestive glands. Differential changes in metabolic biomarkers observed at lower salinity (similar to 23.3) included elevation of succinate, taurine and ATP, and depletion of branched-chain amino acids, threonine and glutamine. Unique effects of arsenic at the lowest salinity (similar to 15.6) included down-regulation of glutamate, succinate and ADP, and up-regulation of phosphocholine. We conclude that salinity influences the metabolic responses of this clam to arsenic.; Ruditapes philippinarum, a clam that thrives in intertidal zones of various salinities, is a useful biomonitor to marine contaminants. We investigated the influence of dilution to 75% and 50% of normal seawater salinity (31.1) on the responses of the digestive gland of R. philippinarum to arsenic exposure (20 mu g/L), using nuclear magnetic resonance (NMR)-based metabolomics. After acute arsenic exposure for 48 h, salinity-dependent differential metabolic responses were detected. In normal seawater, arsenic exposure increased the concentrations of branched-chain amino acids, and of threonine, proline, phosphocholine and adenosine, and it decreased the levels of alanine, hypotaurine, glucose, glycogen and ATP in the digestive glands. Differential changes in metabolic biomarkers observed at lower salinity (similar to 23.3) included elevation of succinate, taurine and ATP, and depletion of branched-chain amino acids, threonine and glutamine. Unique effects of arsenic at the lowest salinity (similar to 15.6) included down-regulation of glutamate, succinate and ADP, and up-regulation of phosphocholine. We conclude that salinity influences the metabolic responses of this clam to arsenic.
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
KeywordRuditapes Philippinarum Salinity Arsenic Toxicological Effects Nuclear Magnetic Resonance (Nmr) Metabolomics
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaLimnology ; Oceanography
Funding OrganizationNational Natural Science Foundation of China [41106102]; Natural Science Foundation of Shandong Province [ZR2009CZ008]; 100 Talents Program of Chinese Academy of Sciences
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Indexed BySCI
WOS KeywordNUCLEAR-MAGNETIC-RESONANCE ; NMR-BASED METABOLOMICS ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; PATTERN-RECOGNITION ; H-1-NMR SPECTROSCOPY ; TAPES-PHILIPPINARUM ; MANILA CLAMS ; RESPONSES ; METABONOMICS ; TOXICITY
SubtypeArticle
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS IDWOS:000316018300010
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6678
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China
2.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
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Ji Chenglong,Wu Huifeng,Liu Xiaoli,et al. The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):345-352.
APA Ji Chenglong,Wu Huifeng,Liu Xiaoli,Zhao Jianmin,Yu Junbao,&Yin Xiuli.(2013).The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),345-352.
MLA Ji Chenglong,et al."The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):345-352.
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