SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres

	SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres
	Ko, Juhui 1; Lee, Sangyeop 1; Lee, Eun Kyu 2; Chang, Soo-Ik 3; Chen, Lingxin4 ; Yoon, Soo-Young 5; Choo, Jaebum 1
发表期刊	PHYSICAL CHEMISTRY CHEMICAL PHYSICS
ISSN	1463-9076
	2013
卷号	15 期号:15 页码:5379-5385
关键词	Enhanced Raman-scattering Patterned Microarray Chip Cancer Markers Nanoprobes Sensors Cells Lung Biosensors System Growth
产权排序	[Ko, Juhui; Lee, Sangyeop; Choo, Jaebum] Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea; [Lee, Eun Kyu] Gacheon Univ, Coll Bionanotechnol, Songnam 461701, South Korea; [Chang, Soo-Ik] Chungbuk Natl Univ, Dept Biochem, Cheongju 361763, South Korea; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Yoon, Soo-Young] Korea Univ, Coll Med, Dept Lab Med, Ansan 425707, South Korea
通讯作者	Yoon, SY (reprint author), Korea Univ, Coll Med, Dept Lab Med, Ansan 425707, South Korea. jbchoo@hanyang.ac.kr
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	A novel SERS-based sandwich immunoassay using DNA aptamers, silica-encapsulated hollow gold nanospheres (SEHGNs) and a gold-patterned microarray was developed for sensitive detection of VEGF (vascular endothelial growth factor) angiogenesis protein markers. Here, a DNA aptamer conjugated to SEHGN was used as a highly reproducible SERS-encoding nanoprobe, and a hybrid microarray including hydrophilic gold wells and other hydrophobic areas was used as a SERS substrate. Target specific DNA aptamers that fold into a G-quadruplex structure were used as a target recognition unit instead of VEGF antibodies. The detection sensitivity was increased by 2 or 3 orders of magnitude over the conventional ELISA method. In particular, the dynamic concentration range was 3 or 4 orders of magnitude greater than that of conventional ELISA. The results demonstrate that this sensing strategy using DNA aptamers is a powerful platform for the design of novel immune-sensors with high performance. In particular, SERS-based detection using SEHGNs provides great promise for highly sensitive biomarker sensing with unprecedented advantages.; A novel SERS-based sandwich immunoassay using DNA aptamers, silica-encapsulated hollow gold nanospheres (SEHGNs) and a gold-patterned microarray was developed for sensitive detection of VEGF (vascular endothelial growth factor) angiogenesis protein markers. Here, a DNA aptamer conjugated to SEHGN was used as a highly reproducible SERS-encoding nanoprobe, and a hybrid microarray including hydrophilic gold wells and other hydrophobic areas was used as a SERS substrate. Target specific DNA aptamers that fold into a G-quadruplex structure were used as a target recognition unit instead of VEGF antibodies. The detection sensitivity was increased by 2 or 3 orders of magnitude over the conventional ELISA method. In particular, the dynamic concentration range was 3 or 4 orders of magnitude greater than that of conventional ELISA. The results demonstrate that this sensing strategy using DNA aptamers is a powerful platform for the design of novel immune-sensors with high performance. In particular, SERS-based detection using SEHGNs provides great promise for highly sensitive biomarker sensing with unprecedented advantages.
文章类型	Article
资助机构	National Research Foundation of Korea [R11-2008-0061852, K20904000004-11A0500-00410]; Nano Material Technology Development Program through the National Research Foundation of Korea; Ministry of Education, Science and Technology [2012035286]
收录类别	SCI
语种	英语
关键词[WOS]	ENHANCED RAMAN-SCATTERING ; PATTERNED MICROARRAY CHIP ; CANCER MARKERS ; NANOPROBES ; SENSORS ; CELLS ; LUNG ; BIOSENSORS ; SYSTEM ; GROWTH
研究领域[WOS]	Chemistry ; Physics
WOS记录号	WOS:000316467800012
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6666
专题	中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea 2.Gacheon Univ, Coll Bionanotechnol, Songnam 461701, South Korea 3.Chungbuk Natl Univ, Dept Biochem, Cheongju 361763, South Korea 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 5.Korea Univ, Coll Med, Dept Lab Med, Ansan 425707, South Korea
推荐引用方式 GB/T 7714	Ko, Juhui,Lee, Sangyeop,Lee, Eun Kyu,et al. SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2013,15(15):5379-5385.
APA	Ko, Juhui.,Lee, Sangyeop.,Lee, Eun Kyu.,Chang, Soo-Ik.,Chen, Lingxin.,...&Choo, Jaebum.(2013).SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres.PHYSICAL CHEMISTRY CHEMICAL PHYSICS,15(15),5379-5385.
MLA	Ko, Juhui,et al."SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres".PHYSICAL CHEMISTRY CHEMICAL PHYSICS 15.15(2013):5379-5385.