| Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis | |
Wang Qing1 ; Ning Xuanxuan2; Pei Dong3; Zhao Jianmin1; You Liping1; Wang Chunyan1; Wu Huifeng1
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| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 2013-05-01 | |
| 卷号 | 31期号:3页码:493-503 |
| 关键词 | Thioredoxin Mussel Bacterial Challenge Antioxidant |
| 产权排序 | [Wang Qing; Zhao Jianmin; You Liping; Wang Chunyan; Wu Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Ning Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Pei Dong] China Agr Univ, Yantai 264670, Peoples R China |
| 通讯作者 | Zhao, JM (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Thioredoxin (Trx) proteins are involved in many biological processes especially the regulation of cellular redox homeostasis. In this study, two Trx cDNAs were cloned from the mussel Mytilus galloprovincialis using rapid amplifi cation of cDNA ends-polymerase chain reaction (RACE-PCR). The two cDNAs were named MgTrx1 and MgTrx2, respectively. The open reading frames of MgTrx1 and MgTrx2 were 318 and 507 base pairs (bp) and they encoded proteins of 105 and 168 amino acids with estimated molecular masses of 11.45 and 18.93 kDa, respectively. Sequence analysis revealed that both proteins possessed the conserved active site dithiol motif Cys-Gly-Pro-Cys. In addition, MgTrx2 also possessed a putative mitochondrial targeting signal suggesting that it is located in the mitochondria. Quantitative real-time polymerase chain reaction (qPCR) revealed that both MgTrx1 and MgTrx2 were constitutively expressed in all tissues examined. The MgTrx1 transcript was most abundant in hemocytes and gills, whereas the MgTrx2 transcript was most abundant in gonad, hepatopancreas, gill and hemocytes. Following Vibrio anguillarum challenge, the expression of MgTrx1 was up-regulated and reached its peak, at a value 10-fold the initial value, at 24 h. Subsequently, expression returned back to the original level. In contrast, the expression level of MgTrx2 was down-regulated following bacterial stimulation, with one fi fth of the control level evident at 12 h post challenge. These results suggest that MgTrx1 and MgTrx2 may play important roles in the response of M. galloprovincialis to bacterial challenge.; Thioredoxin (Trx) proteins are involved in many biological processes especially the regulation of cellular redox homeostasis. In this study, two Trx cDNAs were cloned from the mussel Mytilus galloprovincialis using rapid amplifi cation of cDNA ends-polymerase chain reaction (RACE-PCR). The two cDNAs were named MgTrx1 and MgTrx2, respectively. The open reading frames of MgTrx1 and MgTrx2 were 318 and 507 base pairs (bp) and they encoded proteins of 105 and 168 amino acids with estimated molecular masses of 11.45 and 18.93 kDa, respectively. Sequence analysis revealed that both proteins possessed the conserved active site dithiol motif Cys-Gly-Pro-Cys. In addition, MgTrx2 also possessed a putative mitochondrial targeting signal suggesting that it is located in the mitochondria. Quantitative real-time polymerase chain reaction (qPCR) revealed that both MgTrx1 and MgTrx2 were constitutively expressed in all tissues examined. The MgTrx1 transcript was most abundant in hemocytes and gills, whereas the MgTrx2 transcript was most abundant in gonad, hepatopancreas, gill and hemocytes. Following Vibrio anguillarum challenge, the expression of MgTrx1 was up-regulated and reached its peak, at a value 10-fold the initial value, at 24 h. Subsequently, expression returned back to the original level. In contrast, the expression level of MgTrx2 was down-regulated following bacterial stimulation, with one fi fth of the control level evident at 12 h post challenge. These results suggest that MgTrx1 and MgTrx2 may play important roles in the response of M. galloprovincialis to bacterial challenge. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [31172388]; 100 Talents Program of the Chinese Academy of Sciences; Key Laboratory for Ecological Environment in Coastal Areas, State Oceanic Administration [201011] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | DNA PROTECTION ACTIVITY ; REACTIVE OXYGEN ; MITOCHONDRIAL THIOREDOXIN-2 ; RUDITAPES-PHILIPPINARUM ; RECOMBINANT PROTEIN ; DEFENSE-MECHANISMS ; IMMUNE-RESPONSE ; CELL-GROWTH ; ACTIVATION ; APOPTOSIS |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| WOS记录号 | WOS:000318719700003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6634 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.China Agr Univ, Yantai 264670, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Qing,Ning Xuanxuan,Pei Dong,et al. Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(3):493-503. |
| APA | Wang Qing.,Ning Xuanxuan.,Pei Dong.,Zhao Jianmin.,You Liping.,...&Wu Huifeng.(2013).Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(3),493-503. |
| MLA | Wang Qing,et al."Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.3(2013):493-503. |
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