Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis
Wang, Qing1,2; Yuan, Zeyi3; Wu, Huifeng1,2; Liu, Feng4; Zhao, Jianmin1,2
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2013-05-01
卷号34期号:5页码:1345-1351
关键词Superoxide Dismutase Mytilus Galloprovincialis Bacterial Challenge Immune Responses
产权排序[Wang, Qing; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Wang, Qing; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Yuan, Zeyi] Natl Marine Data & Informat Serv, Tianjin 300171, Peoples R China; [Liu, Feng] China Agr Univ Yantai, Yantai 264670, Peoples R China
通讯作者Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
作者部门中科院海岸带环境过程与生态修复重点实验室
英文摘要The full-length cDNA sequences coding respectively for a manganese superoxide dismutase (Mg-MnSOD) and copper/zinc superoxide dismutase (Mg-CuZnSOD) were cloned from Mytilus galloprovincialis. Mg-MnSOD and Mg-CuZnSOD cDNAs encoded a polypeptide of 228 and 211 amino acids, respectively. Sequence analysis indicated Mg-MnSOD was a mitochondrial MnSOD and Mg-CuZnSOD was an intracellular CuZnSOD. Multiple alignment analysis showed that both Mg-MnSOD and Mg-CuZnSOD sequences had the common features conserved in MnSODs and CuZnSODs, respectively. Phylogenetic analysis revealed that Mg-MnSOD clustered together with MnSODs from other mollusks, whereas Mg-CuZnSOD clustered with other mollusk intracellular CuZnSODs with a wider phylogenetic distance. By quantitative real-time RT-PCR (qPCR) analysis, both Mg-MnSOD and Mg-CuZnSOD transcripts were detected in all tissues examined with the highest expression level in hepatopancreas. Following bacterial challenge, the expression level of Mg-MnSOD and Mg-CuZnSOD increased first and subsequently decreased to the original level in hemocytes. In hepatopancreas, Mg-CuZnSOD mRNA was up-regulated significantly at 72 h and 96 h post challenge, while the level of Mg-MnSOD transcript had no significant change. Therefore, Mg-MnSOD and Mg-CuZnSOD expressions were inducible and they were probably involved in the immune response against bacterial challenge. These results suggest that these SODs may play important roles in the immune defense system of M. galloprovincialis and perhaps contribute to the protective effects against oxidative stress in this mussel. (C) 2013 Elsevier Ltd. All rights reserved.; The full-length cDNA sequences coding respectively for a manganese superoxide dismutase (Mg-MnSOD) and copper/zinc superoxide dismutase (Mg-CuZnSOD) were cloned from Mytilus galloprovincialis. Mg-MnSOD and Mg-CuZnSOD cDNAs encoded a polypeptide of 228 and 211 amino acids, respectively. Sequence analysis indicated Mg-MnSOD was a mitochondrial MnSOD and Mg-CuZnSOD was an intracellular CuZnSOD. Multiple alignment analysis showed that both Mg-MnSOD and Mg-CuZnSOD sequences had the common features conserved in MnSODs and CuZnSODs, respectively. Phylogenetic analysis revealed that Mg-MnSOD clustered together with MnSODs from other mollusks, whereas Mg-CuZnSOD clustered with other mollusk intracellular CuZnSODs with a wider phylogenetic distance. By quantitative real-time RT-PCR (qPCR) analysis, both Mg-MnSOD and Mg-CuZnSOD transcripts were detected in all tissues examined with the highest expression level in hepatopancreas. Following bacterial challenge, the expression level of Mg-MnSOD and Mg-CuZnSOD increased first and subsequently decreased to the original level in hemocytes. In hepatopancreas, Mg-CuZnSOD mRNA was up-regulated significantly at 72 h and 96 h post challenge, while the level of Mg-MnSOD transcript had no significant change. Therefore, Mg-MnSOD and Mg-CuZnSOD expressions were inducible and they were probably involved in the immune response against bacterial challenge. These results suggest that these SODs may play important roles in the immune defense system of M. galloprovincialis and perhaps contribute to the protective effects against oxidative stress in this mussel. (C) 2013 Elsevier Ltd. All rights reserved.
文章类型Article
资助机构NSFC [31172388, 41206105]; Chinese Academy of Sciences
收录类别SCI
语种英语
关键词[WOS]MESSENGER-RNA EXPRESSION ; SCALLOP ARGOPECTEN-IRRADIANS ; VENERUPIS-PHILIPPINARUM ; DEFENSE-MECHANISMS ; OXIDATIVE STRESS ; SOD GENE ; MN-SOD ; CLONING ; IDENTIFICATION ; HEMOCYTES
研究领域[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000317870600040
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6632
专题中国科学院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Natl Marine Data & Informat Serv, Tianjin 300171, Peoples R China
4.China Agr Univ Yantai, Yantai 264670, Peoples R China
推荐引用方式
GB/T 7714
Wang, Qing,Yuan, Zeyi,Wu, Huifeng,et al. Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1345-1351.
APA Wang, Qing,Yuan, Zeyi,Wu, Huifeng,Liu, Feng,&Zhao, Jianmin.(2013).Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis.FISH & SHELLFISH IMMUNOLOGY,34(5),1345-1351.
MLA Wang, Qing,et al."Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1345-1351.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Molecular characteri(1121KB) 开放获取--浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Qing]的文章
[Yuan, Zeyi]的文章
[Wu, Huifeng]的文章
百度学术
百度学术中相似的文章
[Wang, Qing]的文章
[Yuan, Zeyi]的文章
[Wu, Huifeng]的文章
必应学术
必应学术中相似的文章
[Wang, Qing]的文章
[Yuan, Zeyi]的文章
[Wu, Huifeng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Molecular characterization of a manganese superoxide dismutase and copper zinc superoxide dismutase from the mussel Mytilus galloprovincialis.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。