Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol

	Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol
	Zhang, Weiwei 1; Niu, Zongliang 1; Liao, Chunyang1 ; Chen, Lingxin1,2
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
	2013-05-01
卷号	97 期号:9 页码:4095-4104
关键词	Diethylstilbestrol Pseudomonas Sp. Degradation Metabolite Cytotoxicity
产权排序	[Zhang, Weiwei; Niu, Zongliang; Liao, Chunyang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. lxchen@yic.ac.cn
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	Since diethylstilbestrol (DES) interrupts endocrine systems and generates reproductive abnormalities in both wildlife and human beings, methods to remove DES from the environments are urgently recommended. In this study, bacterial strain J51 was isolated and tested to effectively degrade DES. J51 was identified as Pseudomonas sp. based on its nucleotide sequence of 16S rRNA. The quinoprotein alcohol dehydrogenase and isocitrate lyase were identified to be involved in DES degradation by MALDI-TOF-TOF MS/MS analysis. In the presence of 40 mg/l DES, increase of the genes encoding quinoprotein alcohol dehydrogenase and isocitrate lyase in both RNA and protein levels was determined. The HPLC/MS analysis showed that DES was hydrolyzed to a major degrading metabolite DES-4-semiquinone. It was the first time to demonstrate the characteristics of DES degradation by specific bacterial strain and the higher degradation efficiency indicated the potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments.; Since diethylstilbestrol (DES) interrupts endocrine systems and generates reproductive abnormalities in both wildlife and human beings, methods to remove DES from the environments are urgently recommended. In this study, bacterial strain J51 was isolated and tested to effectively degrade DES. J51 was identified as Pseudomonas sp. based on its nucleotide sequence of 16S rRNA. The quinoprotein alcohol dehydrogenase and isocitrate lyase were identified to be involved in DES degradation by MALDI-TOF-TOF MS/MS analysis. In the presence of 40 mg/l DES, increase of the genes encoding quinoprotein alcohol dehydrogenase and isocitrate lyase in both RNA and protein levels was determined. The HPLC/MS analysis showed that DES was hydrolyzed to a major degrading metabolite DES-4-semiquinone. It was the first time to demonstrate the characteristics of DES degradation by specific bacterial strain and the higher degradation efficiency indicated the potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments.
文章类型	Article
资助机构	Innovation Projects of the Chinese Academy of Sciences [KZCX2-EW-206]; National Natural Science Foundation of China (NSFC) [31200041, 20975089, 20907039]; Doctoral Foundation of Shandong Province [BS2011SW056]; Department of Science and Technology of Yantai City of China [2010235]; 100 Talents Program of the Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	BISPHENOL-A ; BIOREMEDIATION ; CHEMICALS ; BACTERIA
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000317681800031
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6624
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weiwei,Niu, Zongliang,Liao, Chunyang,et al. Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(9):4095-4104.
APA	Zhang, Weiwei,Niu, Zongliang,Liao, Chunyang,&Chen, Lingxin.(2013).Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(9),4095-4104.
MLA	Zhang, Weiwei,et al."Isolation and characterization of Pseudomonas sp strain capable of degrading diethylstilbestrol".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.9(2013):4095-4104.

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