Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis | |
Wang, Chunyan1,2; Zhao, Jianmin2 ; Mu, Changkao1; Wang, Qing2; Wu, Huifeng2; Wang, Chunlin1
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2013-02-01 | |
| 卷号 | 34期号:2页码:697-703 |
| 关键词 | Mytilus Galloprovincialis Molecular Cloning Glutathione S-transferases Innate Immune |
| 产权排序 | [Wang, Chunyan; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Wang, Chunyan; Zhao, Jianmin; Wang, Qing; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China |
| 通讯作者 | Mu, CK (reprint author), Ningbo Univ, Sch Marine Sci, 818 Fenghua Rd, Ningbo 315211, Zhejiang, Peoples R China. muchangkao@nbu.edu.cn ; wangchunlin@nbu.edu.cn |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Glutathione S-transferases (GSTs) are phase II enzymes involved in the regulation of redox homeostasis and innate immune responses against bacterial infection, which also play important roles in the detoxification of xenobiotics. In this study, we reported four genes of the GST family (named MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3, respectively) from Mytilus galloprovincialis. MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3 consisted of open reading frame (ORF) of 648 bp, 612 bp, 621 bp and 609 bp respectively, which encoded proteins of 216, 204, 207 and 203 amino acids residues, respectively. Sequence analysis showed that the predicted protein sequence of MgGSTs contained the conserved domain of the GST_N and GST C. Alignment analysis indicated that the MgGSTs were divided into two types, one was of alpha GST, and the others were of sigma class. Tissue distribution study revealed that MgGST alpha, MgGSTS2, MgGSTS3 transcripts were highly expressed in hemocytes, while MgGSTS1 mRNA was most abundantly expressed in hepatopancreas. After bacterial challenge, the expression level of these MgGSTs in hemocytes were all significantly higher than that of the control group. These results suggested that MgGSTs might play important roles in the modulation of immune response in M. galloprovincialis. (C) 2012 Published by Elsevier Ltd.; Glutathione S-transferases (GSTs) are phase II enzymes involved in the regulation of redox homeostasis and innate immune responses against bacterial infection, which also play important roles in the detoxification of xenobiotics. In this study, we reported four genes of the GST family (named MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3, respectively) from Mytilus galloprovincialis. MgGST alpha, MgGSTS1, MgGSTS2, and MgGSTS3 consisted of open reading frame (ORF) of 648 bp, 612 bp, 621 bp and 609 bp respectively, which encoded proteins of 216, 204, 207 and 203 amino acids residues, respectively. Sequence analysis showed that the predicted protein sequence of MgGSTs contained the conserved domain of the GST_N and GST C. Alignment analysis indicated that the MgGSTs were divided into two types, one was of alpha GST, and the others were of sigma class. Tissue distribution study revealed that MgGST alpha, MgGSTS2, MgGSTS3 transcripts were highly expressed in hemocytes, while MgGSTS1 mRNA was most abundantly expressed in hepatopancreas. After bacterial challenge, the expression level of these MgGSTs in hemocytes were all significantly higher than that of the control group. These results suggested that MgGSTs might play important roles in the modulation of immune response in M. galloprovincialis. (C) 2012 Published by Elsevier Ltd. |
| 文章类型 | Article |
| 资助机构 | National Key Technology Support Program [2011BAD13B0903]; K.C. Wong Magna Fund |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MOLECULAR-CLONING ; VENERUPIS-PHILIPPINARUM ; PI-CLASS ; MU-CLASS ; IDENTIFICATION ; HEMOCYTES ; SEQUENCES ; OYSTER ; CLAM |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000315076100035 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6602 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chunyan,Zhao, Jianmin,Mu, Changkao,et al. cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):697-703. |
| APA | Wang, Chunyan,Zhao, Jianmin,Mu, Changkao,Wang, Qing,Wu, Huifeng,&Wang, Chunlin.(2013).cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis.FISH & SHELLFISH IMMUNOLOGY,34(2),697-703. |
| MLA | Wang, Chunyan,et al."cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):697-703. |
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