Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves
Chen, Fujia1,2; Long, Xiaohua1; Yu, Mengni1; Liu, Zhaopu1; Liu, Ling1; Shao, Hongbo1,2,3
2013-05-01
发表期刊INDUSTRIAL CROPS AND PRODUCTS
ISSN0926-6690
卷号47页码:339-345
产权排序[Chen, Fujia; Long, Xiaohua; Yu, Mengni; Liu, Zhaopu; Liu, Ling; Shao, Hongbo] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China; [Chen, Fujia; Shao, Hongbo] Chinese Acad Sci, Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China; [Shao, Hongbo] QUST, Inst Life Sci, Qingdao 266042, Peoples R China
摘要Spoilage of industrial fruits and vegetables in storage and transportation due to fungal infection results in significant losses, and new natural antifungal treatments would have a large economic value. The extracts of antifungal compounds and phenolic acids from Jerusalem artichoke (Helianthus tuberosus L.) leaves were investigated for potential use in enhancing preservation of fruits and vegetables in storage. Either crude leaf extract or n-butanol fraction was active against Botrytis cinerea, Colletotrichum gloeosporioides, Phytophthora capsici Leonian and Rhizoctonia cerealis, with the values of IC50 (half of the maximum inhibitory concentration) ranging from 2466 to 2.534 g L-1 for the crude leaf extract and 0.232-1.911 g L-1 for n-butanol fraction. The severity of grey mould caused by B. cinerea was significantly reduced by n-butanol fraction applied at I and 2 g L-1 (the control efficiency of 71.3% and 77.8%, respectively, compared with commercial preparation Carbendazim. Following in vitro activity-guided fractionation by bioautography, six phenolic acids were separated from n-butanol fraction. Among them, caffeic acid, 3,4-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid played a dominant role and were active in bioassays against Gibberella zeae, with respective minimum inhibitory concentrations (MIC) being 108, 60 and 4.2 mu g mL(-1). These results imply that Jerusalem artichoke leaves might be a potential source of natural fungicides. (c) 2013 Elsevier B.V. All rights reserved.; Spoilage of industrial fruits and vegetables in storage and transportation due to fungal infection results in significant losses, and new natural antifungal treatments would have a large economic value. The extracts of antifungal compounds and phenolic acids from Jerusalem artichoke (Helianthus tuberosus L.) leaves were investigated for potential use in enhancing preservation of fruits and vegetables in storage. Either crude leaf extract or n-butanol fraction was active against Botrytis cinerea, Colletotrichum gloeosporioides, Phytophthora capsici Leonian and Rhizoctonia cerealis, with the values of IC50 (half of the maximum inhibitory concentration) ranging from 2466 to 2.534 g L-1 for the crude leaf extract and 0.232-1.911 g L-1 for n-butanol fraction. The severity of grey mould caused by B. cinerea was significantly reduced by n-butanol fraction applied at I and 2 g L-1 (the control efficiency of 71.3% and 77.8%, respectively, compared with commercial preparation Carbendazim. Following in vitro activity-guided fractionation by bioautography, six phenolic acids were separated from n-butanol fraction. Among them, caffeic acid, 3,4-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid played a dominant role and were active in bioassays against Gibberella zeae, with respective minimum inhibitory concentrations (MIC) being 108, 60 and 4.2 mu g mL(-1). These results imply that Jerusalem artichoke leaves might be a potential source of natural fungicides. (c) 2013 Elsevier B.V. All rights reserved.
通讯作者Liu, L (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China. liuling@njau.edu.cn ; shaohongbochu@126.com
关键词Helianthus Tuberosus Jerusalem Artichoke Antifungal Activity Phenolic Acids Dicaffeoylquinic Acids
作者部门海岸带生物学与生物资源利用所重点实验室
学科领域Agricultural Engineering ; Agronomy
项目资助者Jiangsu Agricultural Science and Technology Independent Innovation Fund Project [CX(12)1005-6]; National Natural Science Foundation of China [31201692, 41171216]; National Key Projects of Scientific and Technical Support Program; Ministry of Science and Technology of China [2011BAD13B09]; Ministry of Science and Technology of Jiangsu Province [BE2011368]; Fundamental Research Funds for Central Universities [Y0201100249]; New Teachers' Fund for Doctor Stations, Ministry of Education [20100097120016]; Project of a Special Fund for Public Welfare Industrial (Agriculture) Research of China [200903001-5]; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS); Project of Shandong Provincial Technology Development plan [2010GSF10208]; CAS/SAFEA International Partnership Program for Creative Research Teams; Science and Technology Development Plan of Yantai City [2011016]; Yantai Double hundred High-end Talent Plan [XY-003-02]; Development Plan of YIC-CAS [135]
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收录类别SCI
关键词[WOS]IN-VITRO ; CONSTITUENTS ; PLANTS ; IDENTIFICATION ; AGRICULTURE ; STORAGE ; STRESS ; ANNUUS ; FUNGI ; ACIDS
文章类型Article
语种英语
WOS研究方向Agriculture
WOS记录号WOS:000319892000047
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6447
专题海岸带生物学与生物资源利用所重点实验室
作者单位1.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Sci, Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China
3.QUST, Inst Life Sci, Qingdao 266042, Peoples R China
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Chen, Fujia,Long, Xiaohua,Yu, Mengni,et al. Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves[J]. INDUSTRIAL CROPS AND PRODUCTS,2013,47:339-345.
APA Chen, Fujia,Long, Xiaohua,Yu, Mengni,Liu, Zhaopu,Liu, Ling,&Shao, Hongbo.(2013).Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves.INDUSTRIAL CROPS AND PRODUCTS,47,339-345.
MLA Chen, Fujia,et al."Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves".INDUSTRIAL CROPS AND PRODUCTS 47(2013):339-345.
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