Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation
Chen Huaxin1; Lin Hanzhi1,2; Jiang Peng1; Li Fuchao1; Qin Song3
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
2013-03-01
卷号31期号:2页码:416-420
关键词Chromatic Acclimation Electroporation Genetic Transformation Synechococcus Sp Cc9311
产权排序[Chen Huaxin; Lin Hanzhi; Jiang Peng; Li Fuchao] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China; [Lin Hanzhi] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者Jiang, P (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. jiangpeng@qdio.ac.cn
作者部门海岸带生物学与生物资源利用所重点实验室
英文摘要Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.; Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
文章类型Article
资助机构Key Innovation Project of Institute of Oceanology, Chinese Academy of Sciences [2009-2]; Natural Science Foundation of Shandong Province [2009ZRB02542]; Foundation of Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques, SOA [MBSMAT-2010-03]; National Natural Science Foundation of China [41276164]; Natural Science Foundation of Jiangsu Province [BK2012650]
收录类别SCI
语种英语
关键词[WOS]CHROMATIC ADAPTATION ; MANIPULATION ; DIVERSITY ; MOTILITY ; STRAINS ; GENUS
研究领域[WOS]Marine & Freshwater Biology ; Oceanography
WOS记录号WOS:000316018300018
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6440
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
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Chen Huaxin,Lin Hanzhi,Jiang Peng,et al. Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):416-420.
APA Chen Huaxin,Lin Hanzhi,Jiang Peng,Li Fuchao,&Qin Song.(2013).Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),416-420.
MLA Chen Huaxin,et al."Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):416-420.
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