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Transcriptomic and Genetic Analysis of Direct Interspecies Electron Transfer
Shrestha, Pravin Malla ; Rotaru, Amelia-Elena ; Summers, Zarath M. ; Shrestha, Minita ; Liu, Fanghua ; Lovley, Derek R.
通讯作者Shrestha, PM (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA.,pravin@microbio.umass.edu
学科主题Biotechnology & Applied Microbiology ; Microbiology
关键词Fe(Iii) Oxide Reduction C-type Cytochrome Geobacter-sulfurreducens Pelobacter-carbinolicus Expression Patterns Anaerobic-bacteria Fuel-cells Rna-seq Genome Metabolism
出处APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN号0099-2240
2013
79期:7页:2397-2404
收录类别SCI
产权排序[Shrestha, Pravin Malla; Rotaru, Amelia-Elena; Summers, Zarath M.; Shrestha, Minita; Liu, Fanghua; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA
资助信息Office of Science (BER), U.S. Department of Energy [DESC0004485]
英文摘要The possibility that metatranscriptomic analysis could distinguish between direct interspecies electron transfer (DIET) and H-2 interspecies transfer (HIT) in anaerobic communities was investigated by comparing gene transcript abundance in cocultures in which Geobacter sulfurreducens was the electron-accepting partner for either Geobacter metallireducens, which performs DIET, or Pelobacter carbinolicus, which relies on HIT. Transcript abundance for G. sulfurreducens uptake hydrogenase genes was 7-fold lower in cocultures with G. metallireducens than in cocultures with P. carbinolicus, consistent with DIET and HIT, respectively, in the two cocultures. Transcript abundance for the pilus-associated cytochrome OmcS, which is essential for DIET but not for HIT, was 240-fold higher in the cocultures with G. metallireducens than in cocultures with P. carbinolicus. The pilin gene pilA was moderately expressed despite a mutation that might be expected to repress pilA expression. Lower transcript abundance for G. sulfurreducens genes associated with acetate metabolism in the cocultures with P. carbinolicus was consistent with the repression of these genes by H2 during HIT. Genes for the biogenesis of pili and flagella and several c-type cytochrome genes were among the most highly expressed in G. metallireducens. Mutant strains that lacked the ability to produce pili, flagella, or the outer surface c-type cytochrome encoded by Gmet_2896 were not able to form cocultures with G. sulfurreducens. These results demonstrate that there are unique gene expression patterns that distinguish DIET from HIT and suggest that metatranscriptomics may be a promising route to investigate interspecies electron transfer pathways in more-complex environments.
文献类型共享期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6307
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Shrestha, Pravin Malla,Rotaru, Amelia-Elena,Summers, Zarath M.,et al. Transcriptomic and Genetic Analysis of Direct Interspecies Electron Transfer. 2013.
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