| A four-domain Kunitz-type proteinase inhibitor from Solen grandis is implicated in immune response | |
Wei, Xiumei2; Yang, Jialong1; Yang, Jianmin2; Liu, Xiangquan2; Liu, Meijun2; Yang, Dinglong2 ; Xu, Jie2; Hu, Xiaoke1
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2012-12-01 | |
| 卷号 | 33期号:6页码:1276-1284 |
| 关键词 | Solen Grandis Kunitz-type Proteinase Inhibitor Innate Immunity Real-time Pcr Inhibitory Activity |
| 产权排序 | [Yang, Jialong; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Yang, Jianmin; Liu, Xiangquan; Liu, Meijun; Yang, Dinglong; Xu, Jie] Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China |
| 通讯作者 | Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Serine proteinase inhibitor (SPI) serves as a negative regulator in immune signal pathway by restraining the activities of serine proteinase (SP) and plays an essential role in the innate immunity. In the present study, a Kunitz-type SPI was identified from the mollusk razor clam Solen grandis (designated as SgKunitz). The full-length cDNA of SgKunitz was of 1284 bp, containing an open reading frame (ORF) of 768 bp. The ORF encoded four Kunitz domains, and their amino acids were well conserved when compared with those in other Kunitz-type SPIs, especially the six cysteines involved in forming of three disulfide bridges in each domain. In addition, the tertiary structure of all the four domains adopted a typical model of Kunitz-type SPI family, indicating SgKunitz was a new member of Kunitz-type SPI superfamily. The mRNA transcripts of SgKunitz were detected in all tested tissues of razor clam, including muscle, mantle, gonad, gill, hepatopancreas and hemocytes, and with the highest expression level in gill. When the razor clams were stimulated by LPS, PGN or beta-1, 3-glucan, the expression level of SgKunitz mRNA in hemocytes was significantly up-regulated (P < 0.01), suggesting SgKunitz might involved in the processes of inhibiting the activity of SPs during the immune responses triggered by various pathogens. Furthermore, the recombinant protein of SgKunitz could effectively inhibit the activities of SP trypsin and chymotrypsin in vitro. The present results suggested SgKunitz could serve as an inhibitor of SP involving in the immune response of S. grandis, and provided helpful evidences to understand the regulation mechanism of immune signal pathway in mollusk. (C) 2012 Elsevier Ltd. All rights reserved.; Serine proteinase inhibitor (SPI) serves as a negative regulator in immune signal pathway by restraining the activities of serine proteinase (SP) and plays an essential role in the innate immunity. In the present study, a Kunitz-type SPI was identified from the mollusk razor clam Solen grandis (designated as SgKunitz). The full-length cDNA of SgKunitz was of 1284 bp, containing an open reading frame (ORF) of 768 bp. The ORF encoded four Kunitz domains, and their amino acids were well conserved when compared with those in other Kunitz-type SPIs, especially the six cysteines involved in forming of three disulfide bridges in each domain. In addition, the tertiary structure of all the four domains adopted a typical model of Kunitz-type SPI family, indicating SgKunitz was a new member of Kunitz-type SPI superfamily. The mRNA transcripts of SgKunitz were detected in all tested tissues of razor clam, including muscle, mantle, gonad, gill, hepatopancreas and hemocytes, and with the highest expression level in gill. When the razor clams were stimulated by LPS, PGN or beta-1, 3-glucan, the expression level of SgKunitz mRNA in hemocytes was significantly up-regulated (P < 0.01), suggesting SgKunitz might involved in the processes of inhibiting the activity of SPs during the immune responses triggered by various pathogens. Furthermore, the recombinant protein of SgKunitz could effectively inhibit the activities of SP trypsin and chymotrypsin in vitro. The present results suggested SgKunitz could serve as an inhibitor of SP involving in the immune response of S. grandis, and provided helpful evidences to understand the regulation mechanism of immune signal pathway in mollusk. (C) 2012 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Program of Agriculture Thoroughbred Project, Shandong province, China; NSFC [31202025]; Research Award Fund for Outstanding Young Scientists of Shandong Province [Y231041011]; Taishan Scholar position funding of Aquatic animal nutrition and feed |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PEPTIDOGLYCAN RECOGNITION PROTEIN ; SCALLOP CHLAMYS-FARRERI ; AMINO-ACID-SEQUENCE ; SERINE-PROTEASE ; TRYPSIN-INHIBITOR ; MOLECULAR-CLONING ; PURIFICATION ; EXPRESSION ; ACTIVATION ; INFECTION |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000311015300006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6082 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Xiumei,Yang, Jialong,Yang, Jianmin,et al. A four-domain Kunitz-type proteinase inhibitor from Solen grandis is implicated in immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(6):1276-1284. |
| APA | Wei, Xiumei.,Yang, Jialong.,Yang, Jianmin.,Liu, Xiangquan.,Liu, Meijun.,...&Hu, Xiaoke.(2012).A four-domain Kunitz-type proteinase inhibitor from Solen grandis is implicated in immune response.FISH & SHELLFISH IMMUNOLOGY,33(6),1276-1284. |
| MLA | Wei, Xiumei,et al."A four-domain Kunitz-type proteinase inhibitor from Solen grandis is implicated in immune response".FISH & SHELLFISH IMMUNOLOGY 33.6(2012):1276-1284. |
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