Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues | |
Wang, Rui; Chen, Lingxin ; Liu, Ping; Zhang, Qin; Wang, Yunqing
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| 发表期刊 | CHEMISTRY-A EUROPEAN JOURNAL
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| ISSN | 0947-6539 |
| 2012-09-01 | |
| 卷号 | 18期号:36页码:11343-11349 |
| 关键词 | Cleavage Reactions Fluorescent Probes Imaging Agents Se-n Bonds Thiols |
| 产权排序 | [Wang, Rui; Chen, Lingxin; Liu, Ping; Zhang, Qin; Wang, Yunqing] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proce, Yantai 264003, Shandong, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proce, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China.,lxchen@yic.ac.cn |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Cy-NiSe and Cy-TfSe were designed and synthesized as sensitive near-infrared (NIR) fluorescent probes for detecting thiols on the basis of Se-N bond cleavage both in cells and in tissues. Since a donor-excited photoinduced electron transfer (d-PET) process occurs between the modulator and the fluorophore, Cy-NiSe and Cy-TfSe have weak fluorescence. On titration with glutathione, the free dye exhibits significant fluorescence enhancement. The two probes are sensitive and selective for thiols over other relevant biological species. They can function rapidly at pH 7.4, and their emission lies in the NIR region. Confocal imaging confirms that Cy-NiSe and Cy-TfSe can be used for detecting thiols in living cells and tissues.; Cy-NiSe and Cy-TfSe were designed and synthesized as sensitive near-infrared (NIR) fluorescent probes for detecting thiols on the basis of Se-N bond cleavage both in cells and in tissues. Since a donor-excited photoinduced electron transfer (d-PET) process occurs between the modulator and the fluorophore, Cy-NiSe and Cy-TfSe have weak fluorescence. On titration with glutathione, the free dye exhibits significant fluorescence enhancement. The two probes are sensitive and selective for thiols over other relevant biological species. They can function rapidly at pH 7.4, and their emission lies in the NIR region. Confocal imaging confirms that Cy-NiSe and Cy-TfSe can be used for detecting thiols in living cells and tissues. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China[20975089, 81102415]; Chinese Academy of Sciences[KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences |
| 收录类别 | SCI ; IC |
| 语种 | 英语 |
| 关键词[WOS] | PHOTOINDUCED ELECTRON-TRANSFER ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; QUINONE MODIFIED ELECTRODE ; GLUTATHIONE-PEROXIDASE ; CAPILLARY-ELECTROPHORESIS ; OXIDATIVE STRESS ; HUMAN PLASMA ; ELECTROCHEMICAL DETECTION ; TOTAL HOMOCYSTEINE ; MASS-SPECTROMETRY |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000308310100025 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6044 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proce, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Rui,Chen, Lingxin,Liu, Ping,et al. Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues[J]. CHEMISTRY-A EUROPEAN JOURNAL,2012,18(36):11343-11349. |
| APA | Wang, Rui,Chen, Lingxin,Liu, Ping,Zhang, Qin,&Wang, Yunqing.(2012).Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues.CHEMISTRY-A EUROPEAN JOURNAL,18(36),11343-11349. |
| MLA | Wang, Rui,et al."Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues".CHEMISTRY-A EUROPEAN JOURNAL 18.36(2012):11343-11349. |
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