YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum
Zhang, Lei1,2,3; Ning, Xuanxuan4; Chen, Leilei5; Li, Chenghua5; Liu, Feng6; Wang, Qing1,2; Wu, Huifeng1,2; Zhao, Jianmin1,2
2012-07-01
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume43Issue:8Pages:1176-1183
Contribution Rank[Zhang, Lei; Wang, Qing; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Coastal Zone Reseach Inst, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Lei; Wang, Qing; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Lei] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Ning, Xuanxuan] Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai, Peoples R China; [Chen, Leilei; Li, Chenghua] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Liu, Feng] China Agr Univ Yantai, Yantai, Peoples R China
AbstractGlutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. It can be divided into selenium-dependent GPx (Se-GPx) and selenium-independent GPx (non-Se-GPx) subfamilies. In this study, the full-length cDNA of a non-Se-GPx was identified from Venerupis philippinarum (denoted as VpGPx) using EST analysis and RACE approaches. The full-length cDNA of VpGPx encoded a polypeptide of 226 amino acids with the predicted molecular mass of 25.41 similar to kDa. Phylogenetic analysis indicated that VpGPx had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the GPx protein family. Spatial expression analysis found that VpGPx transcript was most abundantly expressed in hepatopancreas, gills and haemocytes, and weakly expressed in the tissues of mantle. After Vibrio anguillarum challenge, the expression of VpGPx transcript in overall haemocyte population was down-regulated in the first 6 similar to h, and increased to the peak at 48 similar to h. As time progressed, the expression level dropped to 0.5-fold of the control level at 96 similar to h. All these results indicated that VpGPx was perhaps involved in the immune response against microbe infection and might be contributed to the clearance of bacterial pathogens.; Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. It can be divided into selenium-dependent GPx (Se-GPx) and selenium-independent GPx (non-Se-GPx) subfamilies. In this study, the full-length cDNA of a non-Se-GPx was identified from Venerupis philippinarum (denoted as VpGPx) using EST analysis and RACE approaches. The full-length cDNA of VpGPx encoded a polypeptide of 226 amino acids with the predicted molecular mass of 25.41 similar to kDa. Phylogenetic analysis indicated that VpGPx had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the GPx protein family. Spatial expression analysis found that VpGPx transcript was most abundantly expressed in hepatopancreas, gills and haemocytes, and weakly expressed in the tissues of mantle. After Vibrio anguillarum challenge, the expression of VpGPx transcript in overall haemocyte population was down-regulated in the first 6 similar to h, and increased to the peak at 48 similar to h. As time progressed, the expression level dropped to 0.5-fold of the control level at 96 similar to h. All these results indicated that VpGPx was perhaps involved in the immune response against microbe infection and might be contributed to the clearance of bacterial pathogens.
Corresponding AuthorZhao, JM (reprint author), Chinese Acad Sci, Yantai Coastal Zone Reseach Inst, Key Lab Coastal Zone Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China.,jmzhao@yic.ac.cn
KeywordSelenium-independent Glutathione Peroxidase Venerupis Philippinarum Immune Response Bacterial Challenge
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaFisheries
Funding OrganizationNSFC[30901115]; 100 Talents Program of the Chinese Academy of Sciences; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, SOA[201115]
Indexed BySCI
WOS KeywordMARINE-INVERTEBRATES ; OXIDATIVE STRESS ; GENE-EXPRESSION ; REACTIVE OXYGEN ; UNIO-TUMIDUS ; HOMOLOGY ; INFECTION ; SEQUENCE ; DISEASES ; ENZYMES
SubtypeArticle
Language英语
Funding ProjectZhang, Lei; Ning, Xuanxuan; Chen, Leilei; Li, Chenghua; Liu, Feng; Wang, Qing; Wu, Huifeng; Zhao, Jianmin
WOS Research AreaFisheries
WOS IDWOS:000306184700013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6016
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Coastal Zone Reseach Inst, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
4.Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai, Peoples R China
5.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
6.China Agr Univ Yantai, Yantai, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Lei,Ning, Xuanxuan,Chen, Leilei,et al. Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum[J]. AQUACULTURE RESEARCH,2012,43(8):1176-1183.
APA Zhang, Lei.,Ning, Xuanxuan.,Chen, Leilei.,Li, Chenghua.,Liu, Feng.,...&Zhao, Jianmin.(2012).Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum.AQUACULTURE RESEARCH,43(8),1176-1183.
MLA Zhang, Lei,et al."Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum".AQUACULTURE RESEARCH 43.8(2012):1176-1183.
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