This study aimed to establish an efficient process for separation of phycoerythrin by using Q Sepharose Fast Flow resin and verify its feasibility.[Method] Elution gradient,loading volume and flow rate were optimized to determine the optimal separation condition,under which the scale up process was verified.[Result] The optimal condition for separation of phycoerythrin by using Q Sepharose Fast Flow resin was investigated： 30 ml of laver extract was loaded to a Q Sepharose Fast Flow column with a bed volume of 8 ml;subsequently,the column was eluted under a stepwise gradient elution of 0-0.10-0.35-1.00 mol/L NaCl solution（pH 6.0） at a constant flow rate of 1 ml/min;the elution peak under 0.35 mol/L NaCl solution was collected,and the recovery rate and purity coefficient（A565/A280） of phycoerythrin were determined as 44.3 and 1.15,respectively.Based on the established process,75 ml of phycoerythrin extract was loaded to a Q Sepharose Fast Flow column with bed volume of 20 ml for separation,while no significant variation was observed in separation result.[Conclusion] Phycoerythrin can be well separated from laver extract by Q Sepharose Fast Flow resin and the process is feasible for scale-up.
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