采用可见分光光度法测定螺旋藻藻粉中β-胡萝卜素含量,并对测定过程中有关β-胡萝卜素的提取工艺进行了优化,确定了最佳工艺参数为:提取剂为V(正庚烷):V(丙酮):V(无水乙醇):V(甲苯)=10:7:6:7,提取剂体积为30 mL,浸提时间为12 h,浸提温度为35℃.在该工艺下测得螺旋藻藻粉的β-胡萝卜素含量为134.15 mg/100 g.
中文摘要
In this paper,the beta-carotene of Spirulina Powder was determined by UV - visible spectrophotometry.The process for the extraction of beta - carotene was optimized as followed: n - heptane∶ acetone∶ ethanol∶toluene = 10∶ 7∶ 6∶ 7 as extraction agent,extraction solvent volume 30mL,the extraction time 12h,extraction temperature 35℃.
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