Objective: Bacillus amyloliquefaciens Q-426 could produce varieties of antifungal lipopeptides during its stationary growth process,including bacillomycin D,fengycin A and B. Effects of diketopiperazines ( DKPs) as signal molecules of quorum sensing ( QS) on the biosynthesis of above antifungal compounds were
studied through real time fluorescent quantitative polymerase chain reaction ( Real-time Q-PCR) . Methods: DKPs at a final concentration of 5 mg /l were added to the culture broth of strain Q-426 which was incubated at 30℃ for 12 h. After continuing cultivation for 48 h,quantitative analysis of mRNA expression levels was carried out by Real-time Q-PCR. Result: DKPs could inhibit the expression of some genes related with the biosynthesis of antifungal lipopeptides.
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