Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper
Zhang, Linbao1,2,4; Gan, Junli3; Ke, Changliang3; Liu, Xiaoli1,2,4; Zhao, Jianmin1,2; You, Liping1,2,4; Yu, Junbao1,2; Wu, Huifeng1,2
Source PublicationENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
ISSN1382-6689
2012
Volume33Issue:1Pages:85-91
KeywordVenerupis (Ruditapes) Philippinarum Cytochromes P450 Mrna Expression Benzo[a]Pyrene Heavy Metals Biomarker
Contribution Rank[Zhang, Linbao; Liu, Xiaoli; Zhao, Jianmin; You, Liping; Yu, Junbao; Wu, Huifeng] CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Linbao; Liu, Xiaoli; Zhao, Jianmin; You, Liping; Yu, Junbao; Wu, Huifeng] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Gan, Junli; Ke, Changliang] Key Lab Fishery Ecol & Environm, Guangzhou 510300, Guangdong, Peoples R China; [Zhang, Linbao; Liu, Xiaoli; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Corresponding AuthorZhao, JM (reprint author), CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn
Department污染过程与控制实验室 
AbstractWith the objective to identify promising molecular biomarkers for marine pollution monitoring, a new cytochrome P450 gene was identified from Venerupis (Ruditapes) philippinarum and classified as a member of a new subfamily, CYP414A1. Phylogenetic analysis showed that CYP414A1 was closely related to members of the CYP2 family. CYP414A1 mRNA expression was significantly induced by 50 mu g/L B[a]P at 96h, while no significant change was found in 5 mu g/L B[a]P-exposed samples. For heavy metals exposure, the expression of CYP414A1 was significantly up-regulated by Cd but sharply depressed by Cu exposure. These results suggested that CYP414A1 responded to various xenobiotics stresses, and could be used as a candidate biomarker of heavy metals and B[a]P.; With the objective to identify promising molecular biomarkers for marine pollution monitoring, a new cytochrome P450 gene was identified from Venerupis (Ruditapes) philippinarum and classified as a member of a new subfamily, CYP414A1. Phylogenetic analysis showed that CYP414A1 was closely related to members of the CYP2 family. CYP414A1 mRNA expression was significantly induced by 50 mu g/L B[a]P at 96h, while no significant change was found in 5 mu g/L B[a]P-exposed samples. For heavy metals exposure, the expression of CYP414A1 was significantly up-regulated by Cd but sharply depressed by Cu exposure. These results suggested that CYP414A1 responded to various xenobiotics stresses, and could be used as a candidate biomarker of heavy metals and B[a]P. (C) 2011 Elsevier B.V. All rights reserved.
Funding OrganizationChinese Academy of Sciences[2008GG20005006, 2008GG3NS0700]; SDSFC[ZR2009CZ008]; Key Lab of Fishery Ecology and Environment, Guangdong Province; CAS/SAFEA
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordTIDAL FLATS ; GENE ; MERCURY ; INDUCTION ; CLONING ; MUSSEL ; FISH ; P450
WOS Research AreaEnvironmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
WOS IDWOS:000301876600011
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5609
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
Affiliation1.CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
3.Key Lab Fishery Ecol & Environm, Guangzhou 510300, Guangdong, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Linbao,Gan, Junli,Ke, Changliang,et al. Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2012,33(1):85-91.
APA Zhang, Linbao.,Gan, Junli.,Ke, Changliang.,Liu, Xiaoli.,Zhao, Jianmin.,...&Wu, Huifeng.(2012).Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,33(1),85-91.
MLA Zhang, Linbao,et al."Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 33.1(2012):85-91.
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