| Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene | |
Zhang, Linbao1,3; Qiu, Lihua2; Wu, Huifeng1 ; Liu, Xiaoli1,3; You, Liping1,3; Pei, Dong4; Chen, Leilei1; Wang, Qing1; Zhao, Jianmin1
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| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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| ISSN | 1532-0456 |
| 2012-04-01 | |
| 卷号 | 155期号:3页码:517-527 |
| 关键词 | Venerupis Philippinarum Glutathione S-transferase Heavy Metals Benzo[a]Pyrene Biomarker |
| 产权排序 | [Zhang, Linbao; Wu, Huifeng; Liu, Xiaoli; You, Liping; Chen, Leilei; Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Qiu, Lihua] Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Zhang, Linbao; Liu, Xiaoli; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Pei, Dong] China Agr Univ Yantai, Yantai 264670, Shandong, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. In this study, we reported the cloning and molecular characteristics of seven genes of the GST family (VpGSTS1, VpGSTS2, VpGS7S3, VpGSTO, VpGSTMi, VpGSTM and VpGSTR) from Venerupis philippinarum together with mRNA tissue distribution patterns and temporal expression profiles in response to cadmium, copper and benzo[a]pyrene (B[a]P) exposures. The deduced amino acid sequences of VpGSTs showed high similarities to counterparts of other species that clustered into the same clades in the phylogenetic analysis. At basal levels of tissue expression, most VpGSTs were highly expressed in hepatopancreas compared with other tissues. All VpGSTs showed differential response profiles depending on the concentrations of various toxicants and exposure times. More notably, the expressions of VpGSTS2 and VpGSTS3 transcripts were significantly up-regulated in hepatopancreas from Cu and B[a]P-exposed animals, indicating that these two sigma VpGSTs were highly sensitive to Cu and B[a]P exposure. However, the expressions of VpGSTM and VpGSTR were significantly induced by Cu or B[a]P exposure, respectively. These findings suggested the role of VpGSTS2, VpGSTS3, VpGSTM and VpGSTR in defense against oxidative stress and highlighted their potential as biomarkers to Cu or B[a]P exposure.; Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. In this study, we reported the cloning and molecular characteristics of seven genes of the GST family (VpGSTS1, VpGSTS2, VpGS7S3, VpGSTO, VpGSTMi, VpGSTM and VpGSTR) from Venerupis philippinarum together with mRNA tissue distribution patterns and temporal expression profiles in response to cadmium, copper and benzo[a]pyrene (B[a]P) exposures. The deduced amino acid sequences of VpGSTs showed high similarities to counterparts of other species that clustered into the same clades in the phylogenetic analysis. At basal levels of tissue expression, most VpGSTs were highly expressed in hepatopancreas compared with other tissues. All VpGSTs showed differential response profiles depending on the concentrations of various toxicants and exposure times. More notably, the expressions of VpGSTS2 and VpGSTS3 transcripts were significantly up-regulated in hepatopancreas from Cu and B[a]P-exposed animals, indicating that these two sigma VpGSTs were highly sensitive to Cu and B[a]P exposure. However, the expressions of VpGSTM and VpGSTR were significantly induced by Cu or B[a]P exposure, respectively. These findings suggested the role of VpGSTS2, VpGSTS3, VpGSTM and VpGSTR in defense against oxidative stress and highlighted their potential as biomarkers to Cu or B[a]P exposure. (C) 2012 Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences; Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology[201115]; State Oceanic Administration |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | FISH RIVULUS-MARMORATUS ; HALIOTIS-DISCUS-DISCUS ; MOLECULAR-CLONING ; OMEGA-CLASS ; MYTILUS-GALLOPROVINCIALIS ; AROMATIC-HYDROCARBONS ; METABOLIC-ACTIVATION ; DNA-DAMAGE ; MU-CLASS ; LIVER |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| WOS记录号 | WOS:000301312600011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5605 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.China Agr Univ Yantai, Yantai 264670, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Linbao,Qiu, Lihua,Wu, Huifeng,et al. Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2012,155(3):517-527. |
| APA | Zhang, Linbao.,Qiu, Lihua.,Wu, Huifeng.,Liu, Xiaoli.,You, Liping.,...&Zhao, Jianmin.(2012).Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,155(3),517-527. |
| MLA | Zhang, Linbao,et al."Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 155.3(2012):517-527. |
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