Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis
Wei, Xiumei2; Yang, Jianmin2; Yang, Dinglong2; Xu, Jie2; Liu, Xiangquan2; Yang, Jialong1; Fang, Jinghui2; Qiao, Hongjin2
2012
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号32期号:1页码:178-185
产权排序[Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Yang, Jianmin; Yang, Dinglong; Xu, Jie; Liu, Xiangquan; Fang, Jinghui; Qiao, Hongjin] Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
摘要Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by beta-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly upregulated (P < 0.01) when S. grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of S. grandis, and they might perform different functions in the immune defense against invaders.; Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by beta-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly upregulated (P < 0.01) when S. grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of S. grandis, and they might perform different functions in the immune defense against invaders. (C) 2011 Elsevier Ltd. All rights reserved.
通讯作者Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. lxq6808@163.com ; jlyang@yic.ac.cn
关键词Solen Grandis Pgrp Innate Immunity Pattern Recognition Receptor Real-time Pcr
作者部门生物资源实验室 
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
项目资助者Program of Agriculture Thoroughbred Project, Shandong province, China
URL查看原文
收录类别SCI
关键词[WOS]L-ALANINE AMIDASE ; INNATE IMMUNITY ; DROSOPHILA-MELANOGASTER ; BACTERIAL-INFECTION ; CRASSOSTREA-GIGAS ; CHLAMYS-FARRERI ; PACIFIC OYSTER ; ACTIVATION ; RECEPTOR ; DEFENSE
文章类型Article
语种英语
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000299979100021
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5566
专题海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
推荐引用方式
GB/T 7714
Wei, Xiumei,Yang, Jianmin,Yang, Dinglong,et al. Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(1):178-185.
APA Wei, Xiumei.,Yang, Jianmin.,Yang, Dinglong.,Xu, Jie.,Liu, Xiangquan.,...&Qiao, Hongjin.(2012).Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis.FISH & SHELLFISH IMMUNOLOGY,32(1),178-185.
MLA Wei, Xiumei,et al."Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis".FISH & SHELLFISH IMMUNOLOGY 32.1(2012):178-185.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Molecular cloning an(1580KB) 开放获取--浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wei, Xiumei]的文章
[Yang, Jianmin]的文章
[Yang, Dinglong]的文章
百度学术
百度学术中相似的文章
[Wei, Xiumei]的文章
[Yang, Jianmin]的文章
[Yang, Dinglong]的文章
必应学术
必应学术中相似的文章
[Wei, Xiumei]的文章
[Yang, Jianmin]的文章
[Yang, Dinglong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。