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题名:
Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis
作者: Wei, Xiumei2; Yang, Jianmin2; Yang, Dinglong2; Xu, Jie2; Liu, Xiangquan2; Yang, Jialong1; Fang, Jinghui2; Qiao, Hongjin2
刊名: FISH & SHELLFISH IMMUNOLOGY
ISSN号: 1050-4648
出版日期: 2012
卷号: 32, 期号:1, 页码:178-185
关键词: Solen grandis ; PGRP ; Innate immunity ; Pattern recognition receptor ; Real-time PCR
学科分类: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
产权排序: [Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Yang, Jianmin; Yang, Dinglong; Xu, Jie; Liu, Xiangquan; Fang, Jinghui; Qiao, Hongjin] Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
通讯作者: Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. lxq6808@163.com ; jlyang@yic.ac.cn
作者部门: 生物资源实验室 
中文摘要: Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by beta-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly upregulated (P < 0.01) when S. grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of S. grandis, and they might perform different functions in the immune defense against invaders.
英文摘要: Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by beta-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly upregulated (P < 0.01) when S. grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of S. grandis, and they might perform different functions in the immune defense against invaders. (C) 2011 Elsevier Ltd. All rights reserved.
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]: L-ALANINE AMIDASE ; INNATE IMMUNITY ; DROSOPHILA-MELANOGASTER ; BACTERIAL-INFECTION ; CRASSOSTREA-GIGAS ; CHLAMYS-FARRERI ; PACIFIC OYSTER ; ACTIVATION ; RECEPTOR ; DEFENSE
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000299979100021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/5566
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China

Recommended Citation:
Wei, Xiumei,Yang, Jianmin,Yang, Dinglong,et al. Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(1):178-185.
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