| Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis | |
Zhang, Songyan1; Guo, Huanhuan3; Shi, Fei2,4; Wang, Hui3 ; Li, Lili2; Jiao, Xudong2; Wang, Yipeng2; Yu, Haining1,3
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| 发表期刊 | PEPTIDES
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| ISSN | 0196-9781 |
| 2012-02-01 | |
| 卷号 | 33期号:2页码:251-257 |
| 关键词 | Amphibian Antimicrobial Peptide (Amp) Hainanenins Amolops Hainanensis |
| 产权排序 | [Zhang, Songyan; Yu, Haining] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China; [Shi, Fei; Li, Lili; Jiao, Xudong; Wang, Yipeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China; [Guo, Huanhuan; Wang, Hui; Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China; [Shi, Fei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 通讯作者 | Wang, YP (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China. wyp010@163.com ; joannyu@live.cn |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | Antimicrobial peptides (AMPs) secreted by amphibian skin represent an important innate immune defense strategy. There are more than 340 species in the family of Ranidae worldwidely, and from which nearly 100 families of AMPs comprising between 8 and 48 amino acid (aa) residues have been characterized. In current work, two novel AMPs were purified from the skin secretion of Hainan cascade-frog, Amolops hainanensis, and 31 cDNA sequences encoding 10 novel AMPs belonging to 4 families were cloned from the constructed skin cDNA library of A. hainanensis. Among these 10 AMPs, 5 peptides represent the prototypes of a novel amphibian AMP family. According to the generic name of the species of origin, they were designated as hainanenin-1-5. Each of them consists of 21 aa residues with a C-terminal disulphide loop of 7 residues between Cys(15) and Cys(21). Two of them (hainanenin-1 and 5) were then synthesized and their in vitro activities were screened, including antimicrobial, hemolytic and antioxidant activities. The results showed that hainanenin-1 and 5 possessed strong and broad-spectrum antimicrobial activities against Gram-positive, Gram-negative bacteria and fungi, including a large number of clinically isolated drug-resistant pathogenic microorganisms, and slight antioxidant activity. Undesirably, hainanenin-1 and 5 exhibited strong hemolytic activity on human erythrocytes. The discovery of hainanenins and their great antimicrobial potency provides new templates for anti-infective agent design. Crown Copyright; Antimicrobial peptides (AMPs) secreted by amphibian skin represent an important innate immune defense strategy. There are more than 340 species in the family of Ranidae worldwidely, and from which nearly 100 families of AMPs comprising between 8 and 48 amino acid (aa) residues have been characterized. In current work, two novel AMPs were purified from the skin secretion of Hainan cascade-frog, Amolops hainanensis, and 31 cDNA sequences encoding 10 novel AMPs belonging to 4 families were cloned from the constructed skin cDNA library of A. hainanensis. Among these 10 AMPs, 5 peptides represent the prototypes of a novel amphibian AMP family. According to the generic name of the species of origin, they were designated as hainanenin-1-5. Each of them consists of 21 aa residues with a C-terminal disulphide loop of 7 residues between Cys(15) and Cys(21). Two of them (hainanenin-1 and 5) were then synthesized and their in vitro activities were screened, including antimicrobial, hemolytic and antioxidant activities. The results showed that hainanenin-1 and 5 possessed strong and broad-spectrum antimicrobial activities against Gram-positive, Gram-negative bacteria and fungi, including a large number of clinically isolated drug-resistant pathogenic microorganisms, and slight antioxidant activity. Undesirably, hainanenin-1 and 5 exhibited strong hemolytic activity on human erythrocytes. The discovery of hainanenins and their great antimicrobial potency provides new templates for anti-infective agent design. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chinese National Natural Science Foundation[30900240, 41076098] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SPOTTED TORRENT FROG ; SKIN SECRETIONS ; AMPHIBIAN SKIN ; PEPTIDOMICS ; INHIBITORS ; LOLOENSIS ; SYSTEM |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| WOS记录号 | WOS:000301871200009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5557 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China 3.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Songyan,Guo, Huanhuan,Shi, Fei,et al. Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis[J]. PEPTIDES,2012,33(2):251-257. |
| APA | Zhang, Songyan.,Guo, Huanhuan.,Shi, Fei.,Wang, Hui.,Li, Lili.,...&Yu, Haining.(2012).Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis.PEPTIDES,33(2),251-257. |
| MLA | Zhang, Songyan,et al."Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis".PEPTIDES 33.2(2012):251-257. |
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