| Genomewide Analysis of Carotenoid Cleavage Dioxygenases in Unicellular and Filamentous Cyanobacteria | |
Cui, Hongli1,2; Wang, Yinchu1,2 ; Qin, Song1
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| 发表期刊 | COMPARATIVE AND FUNCTIONAL GENOMICS
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| ISSN | 1531-6912 |
| 2012 | |
| 期号 | .页码:164690 |
| 关键词 | In-vitro Characterization Nostoc Sp Pcc-7120 Abscisic-acid Swiss-model Biochemical-characterization Substrate-specificity Retinal Biosynthesis Oxidative Cleavage Crocus-sativus Yellow Pigment |
| 产权排序 | [Cui, Hongli; Wang, Yinchu; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Coastal Zonebio Resource Lab, Yantai 264003, Peoples R China; [Cui, Hongli; Wang, Yinchu] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Grad Univ, Beijing 100049, Peoples R China |
| 通讯作者 | Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Coastal Zonebio Resource Lab, Yantai 264003, Peoples R China. sqin@yic.ac.cn |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | Carotenoid cleavage dioxygenases (CCDs) are a group of enzymes that catalyze the oxidative cleavage steps from carotenoids to various carotenoid cleavage products. Some ccd genes have been identified and encoded enzymes functionally characterized in many higher plants, but little in cyanobacteria. We performed a comparative analysis of ccd sequences and explored their distribution, classification, phylogeny, evolution, and structure among 37 cyanobacteria. Totally 61 putative ccd sequences were identified, which are abundant in Acaryochloris marina MBIC 11017, filamentous N-2-fixing cyanobacteria, and unicellular cyanobacterial Cyanothece. According to phylogenetic trees of 16S rDNA and CCD, nced and ccd8 genes occur later than the divergence of ccd7, apco, and ccd1. All CCD enzymes share conserved basic structure domains constituted by a single loop formed with seven beta-strands and one helix. In this paper, a general framework of sequence-function-evolution connection for the ccd has been revealed, which may provide new insight for functional investigation.; Carotenoid cleavage dioxygenases (CCDs) are a group of enzymes that catalyze the oxidative cleavage steps from carotenoids to various carotenoid cleavage products. Some ccd genes have been identified and encoded enzymes functionally characterized in many higher plants, but little in cyanobacteria. We performed a comparative analysis of ccd sequences and explored their distribution, classification, phylogeny, evolution, and structure among 37 cyanobacteria. Totally 61 putative ccd sequences were identified, which are abundant in Acaryochloris marina MBIC 11017, filamentous N-2-fixing cyanobacteria, and unicellular cyanobacterial Cyanothece. According to phylogenetic trees of 16S rDNA and CCD, nced and ccd8 genes occur later than the divergence of ccd7, apco, and ccd1. All CCD enzymes share conserved basic structure domains constituted by a single loop formed with seven beta-strands and one helix. In this paper, a general framework of sequence-function-evolution connection for the ccd has been revealed, which may provide new insight for functional investigation. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China[40876082]; Chinese Academy of Sciences; Outstanding Young Scholars Fellowship of Shandong Province[JQ200914] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | IN-VITRO CHARACTERIZATION ; NOSTOC SP PCC-7120 ; ABSCISIC-ACID ; SWISS-MODEL ; BIOCHEMICAL-CHARACTERIZATION ; SUBSTRATE-SPECIFICITY ; RETINAL BIOSYNTHESIS ; OXIDATIVE CLEAVAGE ; CROCUS-SATIVUS ; YELLOW PIGMENT |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS记录号 | WOS:000301263900001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5555 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Coastal Zonebio Resource Lab, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Hongli,Wang, Yinchu,Qin, Song. Genomewide Analysis of Carotenoid Cleavage Dioxygenases in Unicellular and Filamentous Cyanobacteria[J]. COMPARATIVE AND FUNCTIONAL GENOMICS,2012(.):164690. |
| APA | Cui, Hongli,Wang, Yinchu,&Qin, Song.(2012).Genomewide Analysis of Carotenoid Cleavage Dioxygenases in Unicellular and Filamentous Cyanobacteria.COMPARATIVE AND FUNCTIONAL GENOMICS(.),164690. |
| MLA | Cui, Hongli,et al."Genomewide Analysis of Carotenoid Cleavage Dioxygenases in Unicellular and Filamentous Cyanobacteria".COMPARATIVE AND FUNCTIONAL GENOMICS ..(2012):164690. |
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