Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis
Yang, Jialong1,3; Wei, Xiumei2; Liu, Xiangquan2; Xu, Jie2; Yang, Dinglong2; Yang, Jianmin2; Fang, Jinghui2; Hu, Xiaoke1
2012-04-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号32期号:4页码:578-585
产权排序[Yang, Jialong; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Liu, Xiangquan; Xu, Jie; Yang, Dinglong; Yang, Jianmin; Fang, Jinghui] Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China; [Yang, Jialong] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
摘要Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition.; Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition. (C) 2012 Elsevier Ltd. All rights reserved.
通讯作者Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jlyang@yic.ac.cn ; plum0102@gmail.com
关键词Solen Grandis Sialic Acid-binding Lectin Innate Immunity Sugar Recognition Real-time Pcr
作者部门生物资源实验室 
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
项目资助者Program of Agriculture Thoroughbred Project, Shandong province, China
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收录类别SCI
关键词[WOS]ACHATINA-FULICA SNAIL ; C-TYPE LECTIN ; LIMULUS-POLYPHEMUS ; CHLAMYS-FARRERI ; HORSESHOE-CRAB ; ANTIBACTERIAL ACTIVITY ; LOBSTER AGGLUTININS ; PHOSPHATASES SHP-1 ; CEPAEA-HORTENSIS ; ANIMAL LECTINS
文章类型Article
语种英语
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000301827100009
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文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5550
专题海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Yang, Jialong,Wei, Xiumei,Liu, Xiangquan,et al. Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):578-585.
APA Yang, Jialong.,Wei, Xiumei.,Liu, Xiangquan.,Xu, Jie.,Yang, Dinglong.,...&Hu, Xiaoke.(2012).Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis.FISH & SHELLFISH IMMUNOLOGY,32(4),578-585.
MLA Yang, Jialong,et al."Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis".FISH & SHELLFISH IMMUNOLOGY 32.4(2012):578-585.
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