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题名:
Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis
作者: Yang, Jialong1,3; Wei, Xiumei2; Liu, Xiangquan2; Xu, Jie2; Yang, Dinglong2; Yang, Jianmin2; Fang, Jinghui2; Hu, XiaoKe(胡晓珂)1
刊名: FISH & SHELLFISH IMMUNOLOGY
ISSN号: 1050-4648
出版日期: 2012-04-01
卷号: 32, 期号:4, 页码:578-585
关键词: Solen grandis ; Sialic acid-binding lectin ; Innate immunity ; Sugar recognition ; Real-time PCR
学科分类: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
产权排序: [Yang, Jialong; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Liu, Xiangquan; Xu, Jie; Yang, Dinglong; Yang, Jianmin; Fang, Jinghui] Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China; [Yang, Jialong] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
通讯作者: Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jlyang@yic.ac.cn ; plum0102@gmail.com
作者部门: 生物资源实验室 
中文摘要: Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition.
英文摘要: Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition. (C) 2012 Elsevier Ltd. All rights reserved.
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]: ACHATINA-FULICA SNAIL ; C-TYPE LECTIN ; LIMULUS-POLYPHEMUS ; CHLAMYS-FARRERI ; HORSESHOE-CRAB ; ANTIBACTERIAL ACTIVITY ; LOBSTER AGGLUTININS ; PHOSPHATASES SHP-1 ; CEPAEA-HORTENSIS ; ANIMAL LECTINS
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000301827100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/5550
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China

Recommended Citation:
Yang, Jialong,Wei, Xiumei,Liu, Xiangquan,et al. Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):578-585.
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