| Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis | |
Yang, Jialong1,3; Wei, Xiumei2; Liu, Xiangquan2; Xu, Jie2; Yang, Dinglong2 ; Yang, Jianmin2; Fang, Jinghui2; Hu, Xiaoke1
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2012-04-01 | |
| 卷号 | 32期号:4页码:578-585 |
| 关键词 | Solen Grandis Sialic Acid-binding Lectin Innate Immunity Sugar Recognition Real-time Pcr |
| 产权排序 | [Yang, Jialong; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Liu, Xiangquan; Xu, Jie; Yang, Dinglong; Yang, Jianmin; Fang, Jinghui] Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China; [Yang, Jialong] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 通讯作者 | Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jlyang@yic.ac.cn ; plum0102@gmail.com |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition.; Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition. (C) 2012 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Program of Agriculture Thoroughbred Project, Shandong province, China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ACHATINA-FULICA SNAIL ; C-TYPE LECTIN ; LIMULUS-POLYPHEMUS ; CHLAMYS-FARRERI ; HORSESHOE-CRAB ; ANTIBACTERIAL ACTIVITY ; LOBSTER AGGLUTININS ; PHOSPHATASES SHP-1 ; CEPAEA-HORTENSIS ; ANIMAL LECTINS |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000301827100009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5549 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jialong,Wei, Xiumei,Liu, Xiangquan,et al. Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):578-585. |
| APA | Yang, Jialong.,Wei, Xiumei.,Liu, Xiangquan.,Xu, Jie.,Yang, Dinglong.,...&Hu, Xiaoke.(2012).Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis.FISH & SHELLFISH IMMUNOLOGY,32(4),578-585. |
| MLA | Yang, Jialong,et al."Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis".FISH & SHELLFISH IMMUNOLOGY 32.4(2012):578-585. |
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