Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide
Fu, Xiuli1,3; Chen, Lingxin1; Li, Jinhua1; Lin, Meng1; You, Huiyan2; Wang, Wenhai1
2012-04-15
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume34Issue:1Pages:227-231
Contribution Rank[Fu, Xiuli; Chen, Lingxin; Li, Jinhua; Lin, Meng; Wang, Wenhai] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [You, Huiyan] Dalian Univ Technol, Environm & Chem Engn Coll, Dalian 116622, Peoples R China; [Fu, Xiuli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
AbstractA novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02-0.28 mu g/mL (R = 0.9957), and a limit of detection was 5 ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin.; A novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02-0.28 mu g/mL (R = 0.9957), and a limit of detection was 5 ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin. (c) 2012 Elsevier B.V. All rights reserved.
Corresponding AuthorChen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
KeywordHeparin Gold Nanorods Graphene Oxide Colorimetric Detection
Department环境化学实验室 
Subject AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
Funding OrganizationNational Natural Science Foundation of China[20975089, 21105117, 20875088]; Chinese Academy of Sciences[KZCX2-EW-206]
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Indexed BySCI
WOS KeywordSELECTIVE DETECTION ; QUANTIFICATION ; CHROMATOGRAPHY ; NANOPARTICLES ; GROWTH ; PROBES ; DRUGS
SubtypeArticle
Language英语
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS IDWOS:000302886500035
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5513
Collection中科院海岸带环境过程与生态修复重点实验室_环境化学实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Dalian Univ Technol, Environm & Chem Engn Coll, Dalian 116622, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Fu, Xiuli,Chen, Lingxin,Li, Jinhua,et al. Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide[J]. BIOSENSORS & BIOELECTRONICS,2012,34(1):227-231.
APA Fu, Xiuli,Chen, Lingxin,Li, Jinhua,Lin, Meng,You, Huiyan,&Wang, Wenhai.(2012).Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide.BIOSENSORS & BIOELECTRONICS,34(1),227-231.
MLA Fu, Xiuli,et al."Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide".BIOSENSORS & BIOELECTRONICS 34.1(2012):227-231.
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