Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide
Fu, Xiuli1,3; Chen, Lingxin1; Li, Jinhua1; Lin, Meng1; You, Huiyan2; Wang, Wenhai1
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
2012-04-15
卷号34期号:1页码:227-231
关键词Heparin Gold Nanorods Graphene Oxide Colorimetric Detection
产权排序[Fu, Xiuli; Chen, Lingxin; Li, Jinhua; Lin, Meng; Wang, Wenhai] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [You, Huiyan] Dalian Univ Technol, Environm & Chem Engn Coll, Dalian 116622, Peoples R China; [Fu, Xiuli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
作者部门环境化学实验室 
英文摘要A novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02-0.28 mu g/mL (R = 0.9957), and a limit of detection was 5 ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin.; A novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02-0.28 mu g/mL (R = 0.9957), and a limit of detection was 5 ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin. (c) 2012 Elsevier B.V. All rights reserved.
文章类型Article
资助机构National Natural Science Foundation of China[20975089, 21105117, 20875088]; Chinese Academy of Sciences[KZCX2-EW-206]
收录类别SCI
语种英语
关键词[WOS]SELECTIVE DETECTION ; QUANTIFICATION ; CHROMATOGRAPHY ; NANOPARTICLES ; GROWTH ; PROBES ; DRUGS
研究领域[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS记录号WOS:000302886500035
引用统计
被引频次:121[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5513
专题中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Dalian Univ Technol, Environm & Chem Engn Coll, Dalian 116622, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Fu, Xiuli,Chen, Lingxin,Li, Jinhua,et al. Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide[J]. BIOSENSORS & BIOELECTRONICS,2012,34(1):227-231.
APA Fu, Xiuli,Chen, Lingxin,Li, Jinhua,Lin, Meng,You, Huiyan,&Wang, Wenhai.(2012).Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide.BIOSENSORS & BIOELECTRONICS,34(1),227-231.
MLA Fu, Xiuli,et al."Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide".BIOSENSORS & BIOELECTRONICS 34.1(2012):227-231.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Label-free colorimet(657KB) 开放获取--浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Fu, Xiuli]的文章
[Chen, Lingxin]的文章
[Li, Jinhua]的文章
百度学术
百度学术中相似的文章
[Fu, Xiuli]的文章
[Chen, Lingxin]的文章
[Li, Jinhua]的文章
必应学术
必应学术中相似的文章
[Fu, Xiuli]的文章
[Chen, Lingxin]的文章
[Li, Jinhua]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。