Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge
Zhang, Linbao1,2; Liu, Xiaoli1,2; Chen, Leilei3; You, Liping1,2; Pei, Dong4; Cong, Ming1; Zhao, Jianmin1; Li, Chenghua1; Liu, Dongyan1; Yu, Junbao1; Wu, Huifeng1
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2011-12-01
Volume31Issue:6Pages:831-837
KeywordVenerupis Philippinarum Selenium-dependent Glutathione Peroxidase Bacterial Challenge Heavy Metals Benzo[a]Pyrene
Contribution Rank[Zhang, Linbao; Liu, Xiaoli; You, Liping; Cong, Ming; Zhao, Jianmin; Li, Chenghua; Liu, Dongyan; Yu, Junbao; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Linbao; Liu, Xiaoli; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Chen, Leilei] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China; [Pei, Dong] China Agr Univ Yantai, Yantai 264670, Peoples R China
Corresponding AuthorZhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
Department污染过程与控制实验室 
AbstractGlutathione peroxidases (GPx) are key enzymes in the antioxidant systems of living organisms by catalyzing the reduction of peroxides to non-reactive products. In the present study, the full-length cDNA encoding a selenium-dependent GPx was identified from Venerupis philippinarum (designated as VpSe-GPx), and the spatial and temporal expression patterns post-Vibrio anguillarum, heavy metals and benzo[a]pyrene (B[a]P) challenge were also investigated. VpSe-GPx possessed all the conserved features critical for the fundamental structure and function of glutathione peroxidase. The VpSe-GPx mRNA was found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpSe-GPx, and the highest expression level was detected at 24 h post-infection with 6.5-fold increase compared with that in the control group. For heavy metals exposure, the expression of VpSe-GPx was significantly induced by 20, 40 mu g L(-1) Cd and 10, 20 mu g L(-1) Cu but depressed by 10 mu g L(-1) Cd and 40 mu g L(-1) Cu. With regards to B[a]P exposure, the expression of VpSe-GPx mRNA was significantly induced at 48 and 96 h post challenge. All these results suggested that VpSe-GPx was potentially involved in mediating the immune response and antioxidant defense in V. Philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.; Glutathione peroxidases (GPx) are key enzymes in the antioxidant systems of living organisms by catalyzing the reduction of peroxides to non-reactive products. In the present study, the full-length cDNA encoding a selenium-dependent GPx was identified from Venerupis philippinarum (designated as VpSe-GPx), and the spatial and temporal expression patterns post-Vibrio anguillarum, heavy metals and benzo[a]pyrene (B[a]P) challenge were also investigated. VpSe-GPx possessed all the conserved features critical for the fundamental structure and function of glutathione peroxidase. The VpSe-GPx mRNA was found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpSe-GPx, and the highest expression level was detected at 24 h post-infection with 6.5-fold increase compared with that in the control group. For heavy metals exposure, the expression of VpSe-GPx was significantly induced by 20, 40 mu g L(-1) Cd and 10, 20 mu g L(-1) Cu but depressed by 10 mu g L(-1) Cd and 40 mu g L(-1) Cu. With regards to B[a]P exposure, the expression of VpSe-GPx mRNA was significantly induced at 48 and 96 h post challenge. All these results suggested that VpSe-GPx was potentially involved in mediating the immune response and antioxidant defense in V. Philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordCLAM RUDITAPES-DECUSSATUS ; OXIDATIVE STRESS ; CRASSOSTREA-GIGAS ; REACTIVE OXYGEN ; FREE-RADICALS ; DNA-DAMAGE ; EXPRESSION ; IDENTIFICATION ; ANTIOXIDANTS ; INFECTION
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000298569700014
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5396
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China
4.China Agr Univ Yantai, Yantai 264670, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Linbao,Liu, Xiaoli,Chen, Leilei,et al. Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):831-837.
APA Zhang, Linbao.,Liu, Xiaoli.,Chen, Leilei.,You, Liping.,Pei, Dong.,...&Wu, Huifeng.(2011).Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge.FISH & SHELLFISH IMMUNOLOGY,31(6),831-837.
MLA Zhang, Linbao,et al."Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):831-837.
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