NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc
Wu, Huifeng1,2; Liu, Xiaoli1,2,3; Zhao, Jianmin1,2; Yu, Junbao1,2
2011-09-01
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume9Issue:9Pages:1566-1579
Contribution Rank[Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Yu, Junbao] CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Liu, Xiaoli] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
AbstractManila clam Ruditapes philippinarum is one of the most important economic species in shellfishery in China due to its wide geographic distribution and high tolerance to environmental changes (e. g., salinity, temperature). In addition, Manila clam is a good biomonitor/bioindicator in "Mussel Watch Programs" and marine environmental toxicology. However, there are several pedigrees of R. philippinarum distributed in the marine environment in China. No attention has been paid to the biological differences between various pedigrees of Manila clams, which may introduce undesirable biological variation in toxicology studies. In this study, we applied NMR-based metabolomics to detect the biological differences in two main pedigrees (White and Zebra) of R. philippinarum and their differential responses to heavy metal exposures (Cadmium and Zinc) using adductor muscle as a target tissue to define one sensitive pedigree of R. philippinarum as biomonitor for heavy metals. Our results indicated that there were significant metabolic differences in adductor muscle tissues between White and Zebra clams, including higher levels of alanine, glutamine, hypotaurine, phosphocholine and homarine in White clam muscles and higher levels of branched chain amino acids (valine, leucine and isoleucine), succinate and 4-aminobutyrate in Zebra clam muscles, respectively. Differential metabolic responses to heavy metals between White and Zebra clams were also found. Overall, we concluded that White pedigree of clam could be a preferable bioindicator/biomonitor in marine toxicology studies and for marine heavy metals based on the relatively high sensitivity to heavy metals.; Manila clam Ruditapes philippinarum is one of the most important economic species in shellfishery in China due to its wide geographic distribution and high tolerance to environmental changes (e. g., salinity, temperature). In addition, Manila clam is a good biomonitor/bioindicator in "Mussel Watch Programs" and marine environmental toxicology. However, there are several pedigrees of R. philippinarum distributed in the marine environment in China. No attention has been paid to the biological differences between various pedigrees of Manila clams, which may introduce undesirable biological variation in toxicology studies. In this study, we applied NMR-based metabolomics to detect the biological differences in two main pedigrees (White and Zebra) of R. philippinarum and their differential responses to heavy metal exposures (Cadmium and Zinc) using adductor muscle as a target tissue to define one sensitive pedigree of R. philippinarum as biomonitor for heavy metals. Our results indicated that there were significant metabolic differences in adductor muscle tissues between White and Zebra clams, including higher levels of alanine, glutamine, hypotaurine, phosphocholine and homarine in White clam muscles and higher levels of branched chain amino acids (valine, leucine and isoleucine), succinate and 4-aminobutyrate in Zebra clam muscles, respectively. Differential metabolic responses to heavy metals between White and Zebra clams were also found. Overall, we concluded that White pedigree of clam could be a preferable bioindicator/biomonitor in marine toxicology studies and for marine heavy metals based on the relatively high sensitivity to heavy metals.
Corresponding AuthorWu, HF (reprint author), CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
KeywordRuditapes Philippinarum Heavy Metal Biomonitor Nmr Metabolomics
Department污染过程与控制实验室 
Subject AreaPharmacology & Pharmacy
Funding OrganizationChinese Academy of Sciences[KZCX2-YW-Q07-04]; NSFC[41106102]; SDSFC[ZR2009CZ008]; CAS/SAFEA
URL查看原文
Indexed BySCI
WOS KeywordMAGNETIC-RESONANCE-SPECTROSCOPY ; PATTERN-RECOGNITION ; H-1-NMR SPECTROSCOPY ; TAPES-PHILIPPINARUM ; METABONOMICS ; TISSUE ; TRANSFORMATION ; PERFORMANCE ; LA(NO3)(3) ; DECUSSATUS
SubtypeArticle
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS IDWOS:000298927500010
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5382
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
Affiliation1.CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,et al. NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc[J]. MARINE DRUGS,2011,9(9):1566-1579.
APA Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,&Yu, Junbao.(2011).NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc.MARINE DRUGS,9(9),1566-1579.
MLA Wu, Huifeng,et al."NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc".MARINE DRUGS 9.9(2011):1566-1579.
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