| NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc | |
Wu, Huifeng1,2 ; Liu, Xiaoli1,2,3; Zhao, Jianmin1,2; Yu, Junbao1,2
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| 发表期刊 | MARINE DRUGS
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| ISSN | 1660-3397 |
| 2011-09-01 | |
| 卷号 | 9期号:9页码:1566-1579 |
| 关键词 | Ruditapes Philippinarum Heavy Metal Biomonitor Nmr Metabolomics |
| 产权排序 | [Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Yu, Junbao] CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Liu, Xiaoli] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Manila clam Ruditapes philippinarum is one of the most important economic species in shellfishery in China due to its wide geographic distribution and high tolerance to environmental changes (e. g., salinity, temperature). In addition, Manila clam is a good biomonitor/bioindicator in "Mussel Watch Programs" and marine environmental toxicology. However, there are several pedigrees of R. philippinarum distributed in the marine environment in China. No attention has been paid to the biological differences between various pedigrees of Manila clams, which may introduce undesirable biological variation in toxicology studies. In this study, we applied NMR-based metabolomics to detect the biological differences in two main pedigrees (White and Zebra) of R. philippinarum and their differential responses to heavy metal exposures (Cadmium and Zinc) using adductor muscle as a target tissue to define one sensitive pedigree of R. philippinarum as biomonitor for heavy metals. Our results indicated that there were significant metabolic differences in adductor muscle tissues between White and Zebra clams, including higher levels of alanine, glutamine, hypotaurine, phosphocholine and homarine in White clam muscles and higher levels of branched chain amino acids (valine, leucine and isoleucine), succinate and 4-aminobutyrate in Zebra clam muscles, respectively. Differential metabolic responses to heavy metals between White and Zebra clams were also found. Overall, we concluded that White pedigree of clam could be a preferable bioindicator/biomonitor in marine toxicology studies and for marine heavy metals based on the relatively high sensitivity to heavy metals.; Manila clam Ruditapes philippinarum is one of the most important economic species in shellfishery in China due to its wide geographic distribution and high tolerance to environmental changes (e. g., salinity, temperature). In addition, Manila clam is a good biomonitor/bioindicator in "Mussel Watch Programs" and marine environmental toxicology. However, there are several pedigrees of R. philippinarum distributed in the marine environment in China. No attention has been paid to the biological differences between various pedigrees of Manila clams, which may introduce undesirable biological variation in toxicology studies. In this study, we applied NMR-based metabolomics to detect the biological differences in two main pedigrees (White and Zebra) of R. philippinarum and their differential responses to heavy metal exposures (Cadmium and Zinc) using adductor muscle as a target tissue to define one sensitive pedigree of R. philippinarum as biomonitor for heavy metals. Our results indicated that there were significant metabolic differences in adductor muscle tissues between White and Zebra clams, including higher levels of alanine, glutamine, hypotaurine, phosphocholine and homarine in White clam muscles and higher levels of branched chain amino acids (valine, leucine and isoleucine), succinate and 4-aminobutyrate in Zebra clam muscles, respectively. Differential metabolic responses to heavy metals between White and Zebra clams were also found. Overall, we concluded that White pedigree of clam could be a preferable bioindicator/biomonitor in marine toxicology studies and for marine heavy metals based on the relatively high sensitivity to heavy metals. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences[KZCX2-YW-Q07-04]; NSFC[41106102]; SDSFC[ZR2009CZ008]; CAS/SAFEA |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; PATTERN-RECOGNITION ; H-1-NMR SPECTROSCOPY ; TAPES-PHILIPPINARUM ; METABONOMICS ; TISSUE ; TRANSFORMATION ; PERFORMANCE ; LA(NO3)(3) ; DECUSSATUS |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| WOS记录号 | WOS:000298927500010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5382 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,et al. NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc[J]. MARINE DRUGS,2011,9(9):1566-1579. |
| APA | Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,&Yu, Junbao.(2011).NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc.MARINE DRUGS,9(9),1566-1579. |
| MLA | Wu, Huifeng,et al."NMR-Based Metabolomic Investigations on the Differential Responses in Adductor Muscles from Two Pedigrees of Manila Clam Ruditapes philippinarum to Cadmium and Zinc".MARINE DRUGS 9.9(2011):1566-1579. |
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